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Multiple online detection allows quicker fraction selection

Date: May 22, 2019

Author: Ryan De Vooght-Johnson

The preparative chromatography of biomolecules, such as proteins, typically requires extensive offline analysis of the fractions before fraction pooling and subsequent processing can begin. Scientists from the Austrian Centre of Industrial Biotechnology, the University of Natural Resources and Life Sciences, and Boehringer Ingelheim (Vienna) examined the output from the chromatographic purification of a protein using a large number of different online detectors and constructed suitable models for...

Read More thumbnail image: Multiple online detection allows quicker fraction selection

Quick chromatography characterises coconut compounds

Date: May 21, 2019

Author: Ryan De Vooght-Johnson

The complex mixtures of compounds found in bio-oil samples are often separated by two-dimensional GC (GC × GC), usually involving long run times. Scientists from three Brazilian universities (the Federal University of Rio Grande do Sul, Porto Alegre, Tiradentes University, Aracaju, and the State University of Rio de Janeiro) developed a new, quicker method for bio-oil chromatography using fast GC × GC with a cryogen-free thermal modulator, using it to examine a bio-oil produced from the pyrolysis of...

Read More thumbnail image: Quick chromatography characterises coconut compounds

Journal Highlight: Ethylene glycol: Evidence of glucuronidation in vivo shown by analysis of clinical toxicology samples

Date: May 21, 2019

Author: separationsNOW

The hypothetical human glucuronide metabolite of ethylene glycol (EG) has been detected and measured in serum of patients with EG poisoning by LC/MS and has been proposed as a biomarker for EG intake.

Read More thumbnail image: Journal Highlight Ethylene glycol Evidence of glucuronidation in vivo shown by analysis of clinical toxicology samples

Come together: Two-photon-excited-fluorescence detection with supercritical fluid chromatography

Date: May 20, 2019

Author: Jon Evans

When looking for a detection technique for the first microchip version of supercritical fluid chromatography, German chemists turned to a form of laser-induced fluorescence detection inspired by two-photon fluorescence microscopy.

Read More thumbnail image: Come together Two-photon-excited-fluorescence detection with supercritical fluid chromatography

Milky goodness: A novel method for extracting phytoestrogens from milk

Date: May 20, 2019

Author: Jon Evans

Italian chemists have developed a novel method for extracting and modifying phytoestrogens from both mammalian and vegetable milks for analysis by gas chromatography-mass spectrometry.

Read More thumbnail image: Milky goodness A novel method for extracting phytoestrogens from milk

Playing catch: Concentration and separation in the same capillary

Date: May 20, 2019

Author: Jon Evans

By passing antibody labelled beads back and forth between magnetic tweezers, a team of chemists from France and Switzerland has found a way to concentrate and separate proteins in the same capillary.

Read More thumbnail image: Playing catch Concentration and separation in the same capillary

Honey proteome: Novel allergens and venom-like proteins

Date: May 17, 2019

Author: Steve Down

The protein compositions of 13 different types of honey have been determined by mass spectrometry, revealing strong similarities and several novel proteins which could be associated with the food's curative properties.

Read More thumbnail image: Honey proteome Novel allergens and venom-like proteins

Micelles measure microbe-massacring nanoparticles

Date: May 16, 2019

Author: Ryan De Vooght-Johnson

Silver nanoparticles are sometimes used as antimicrobials in wound dressings, but concerns have been raised that they may delay the healing process by damaging human cells. Scientists from four Polish institutions used micellar electrokinetic chromatography (MEKC), in conjunction with other analytical methods, to examine the release of silver nanoparticles from silver-containing wound dressings. 

Read More thumbnail image: Micelles measure microbe-massacring nanoparticles

Journal Highlight: Metabolic fingerprinting of chorionic villous samples in normal pregnancy and chromosomal disorders

Date: May 14, 2019

Author: separationsNOW

Metabolic changes in chorionic villi during the first trimester of pregnancy in euploid and aneuploid cases have been studied by GC/MS, HPLC and NMR, revealing that polyol pathways, myo‐inositol and oxidative stress may have fundamental roles.

Read More thumbnail image: Journal Highlight Metabolic fingerprinting of chorionic villous samples in normal pregnancy and chromosomal disorders

Anion ammunition: Analyzing explosive residues with ion-exchange chromatography and mass spectrometry

Date: May 13, 2019

Author: Jon Evans

By coupling ion-exchange chromatography with mass spectrometry, British scientists have found a way to derive more information about the materials used to produce explosives and ammunition from their residues.

Read More thumbnail image: Anion ammunition Analyzing explosive residues with ion-exchange chromatography and mass spectrometry
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