Latest webinar from Agilent Technologies

Improving the Analysis of Polar Analytes with Novel HILIC Phases
on Superficially Porous Particle LC Columns


Thursday, February 8, 2018 – 16:00 UTC
8am PST  |  11am EST  |  4pm UK  |  5pm CET

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The rapidly advancing fields of pharmaceuticals, pesticide analysis, and food testing have encountered a major challenge in the separation and quantitation of highly polar analytes. Adequately retaining and separating small polar molecules with reversed-phase liquid chromatography (RPLC) is often a challenging task. Alkyl phase LC columns, like C18, are a common starting point for LC method development, but highly polar analytes are poorly retained on these non-polar stationary phases. Several techniques have been developed to analyze polar compounds: pH adjustment, ion pairing, normal phase chromatography, derivatization, ion chromatography, and hydrophilic interaction chromatography (HILIC). Of these, HILIC has rapidly gained popularity among many chromatographers for its ability to retain and separate polar analytes.

This presentation will address the use and best practices of HILIC stationary phases with regards to optimal polar compound retention. Stationary phases, solvent strength and buffers types will be addressed with respect to their unique impact on hydrophilic interaction chromatography, as well as advantages and disadvantages to the technique. Two novel HILIC chemistries on superficially porous particle columns will demonstrate these method considerations along with technical advancements in the area of highly efficient HILIC separations when compared to traditional HILIC stationary phases.

Key learning objectives:

  • Obtain brief background on HILIC analyses; history, retention mechanism, comparison to RPLC
  • Learn about method development for polar analytes, including unique HILIC method parameters
  • Learn Pros and Cons of the HILIC technique and why HILIC is an ideal choice for polar analytes
  • Discuss various HILIC stationary phases and when to use what
  • Additional tips and tools to ensure a robust method

Who should attend:

  • Lab Managers of contract, government, or QA/QC labs
  • Analytical chemists who develop methods for polar compounds in environmental, pharmaceutical, or food samples
  • Analysts and technicians performing analysis of polar compounds
  • Graduate students and post-docs working on environmental, pharmaceutical, or food analyses


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