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Idiopathic pulmonary fibrosis: New circulatory biomarkers

Date: Sep 4, 2019

Author: Steve Down

Several plasma biomarkers of idiopathic pulmonary fibrosis have been identified and validated by a combination of mass spectrometry methods, which will help to characterise the disease and could lead to better ways of diagnosis.

Read More thumbnail image: Idiopathic pulmonary fibrosis New circulatory biomarkers

Journal Highlight: Strong anion exchange‐mediated phosphoproteomics reveals extensive human non‐canonical phosphorylation

Date: Sep 3, 2019

Author: separationsNOW

An unbiased phosphopeptide enrichment strategy based on strong anion exchange chromatography (UPAX) has been devised for identification of His, Arg, Lys, Asp, Glu and Cys phosphorylation sites on human proteins by mass spectrometry‐based phosphoproteomics.

Read More thumbnail image: Journal Highlight Strong anion exchangemediated phosphoproteomics reveals extensive human noncanonical phosphorylation

Alzheimer's disease symptoms reversed: Therapeutic targeting of overexpressed circadian clock protein

Date: Aug 14, 2019

Author: Steve Down

Therapeutic targeting of casein kinase 1δ and 1ε, which are circadian clock regulators and overexpressed in the brains of Alzheimer's disease brains, reduced their levels in the mouse model to restore normal circadian rhythm and recover memory deficits.

Read More thumbnail image: Alzheimers disease symptoms reversed Therapeutic targeting of overexpressed circadian clock protein

Epsilon toxin: Biomarkers of human infection

Date: Aug 2, 2019

Author: Steve Down

Several protein biomarkers have been proposed for the potential biowarfare agent epsilon toxin after murine experiments, providing potential diagnostic and therapeutic targets for appropriate clinical intervention..

Read More thumbnail image: Epsilon toxin Biomarkers of human infection

Journal Highlight: Degradomics in biomarker discovery

Date: Jul 29, 2019

Author: separationsNOW

Some of the latest developments in degradomic methodologies used for the identification and validation of biologically relevant proteolytic events and their application in the search for clinically relevant biomarker candidates have been presented and the current state of degradomics in clinics has been discussed.

Read More thumbnail image: Journal Highlight Degradomics in biomarker discovery

Gestational diabetes: Diagnostic biomarkers

Date: Jul 18, 2019

Author: Steve Down

A proteomics study has raised a number of candidate proteins for the prediction and diagnosis of gestational diabetes, a condition which affects up to 10% of pregnancies.

Read More thumbnail image: Gestational diabetes Diagnostic biomarkers

Safety testing of GM maize: Combined omics approach

Date: Jul 4, 2019

Author: Steve Down

A combined transcriptomics, proteomics and metabolomics approach has been used in a proof-of-concept to compare genetically modified maize that has been used in feeding trials with non-modified varieties, revealing minimal differences.

Read More thumbnail image: Safety testing of GM maize Combined omics approach

Proteomic performance predictions possible for LC-MS

Date: Jul 3, 2019

Author: Ryan De Vooght-Johnson

The routine use of LC-MS in proteomics has led to a need for appropriate QC software tools to assess LC-MS performance, but existing packages have their limitations. Scientists from the University of Sydney have developed a novel web-based system (QCMAP) for checking the operation of proteomic LC-MS systems and predicting their performance on biological samples.

Read More thumbnail image: Proteomic performance predictions possible for LC-MS

Journal Highlight: Proteome analysis using gel‐LC‐MS/MS

Date: Jul 2, 2019

Author: separationsNOW

Protocols are described for processing protein samples using 1D SDS gels prior to protease digestion for proteomics workflows that subsequently utilize reversed‐phase nanocapillary ultra‐high‐pressure liquid chromatography coupled to tandem mass spectrometry.

Read More thumbnail image: Journal Highlight Proteome analysis using gelLCMSMS

Bi-omics system: Proteomics and metabolomics working together

Date: Jun 20, 2019

Author: Steve Down

A new dual-column LC/MS approach has been used in a bi-omics method to measure the metabolites and tryptic peptides in biological systems, with online optimisation of the collision energies and SRM transitions that was independent of standard compounds.

Read More thumbnail image: Bi-omics system Proteomics and metabolomics working together
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