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Identification and metabolite profiling of alkaloids in aerial parts of Papaver rhoeas by liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry

Published: Apr 25, 2018

Author(s): Jae‐Hyeon Oh, In Jin Ha, Min Young Lee, Eun‐Ok Kim, Dain Park, Jun‐Hee Lee, Seok‐Geun Lee, Do‐Wan Kim, Tae‐Ho Lee, Eui‐Ju Lee, Chang‐Kug Kim

Journal: Journal of Separation Science

Abstract Papaver plants can produce diverse bioactive alkaloids. Papaver rhoeas Linnaeus (common poppy or corn poppy) is an annual flowering medicinal plant used for treating cough, sleep disorder, and as a sedative, pain reliever, and food. It contains various powerful alkaloids like rhoeadine, benzylisoquinoline, and proaporphine. To investigate and identify alkaloids in the aerial parts of P. rhoeas, samples were collected at different...

Read More thumbnail image: Identification and metabolite profiling of alkaloids in aerial parts of Papaver rhoeas by liquid chromatography coupled with quadrupole timeofflight tandem mass spectrometry

Extraction approaches used for the determination of biologically active compounds (cyclitols, polyphenols and saponins) isolated from plant material

Published: Apr 25, 2018

Author(s): Magdalena Ligor, Ileana‐Andreea Ratiu, Anna Kiełbasa, Hossam Al‐Suod, Bogusław Buszewski

Journal: ELECTROPHORESIS

Abstract Based on the bioactive properties of certain compounds, such as antioxidant and anti‐inflammatory activities, an interesting subject of research are natural substances present in various parts of plants. The choice of the most appropriate method for separation and quantification of biologically active compounds from plants and natural products is a crucial step of any analytical procedure. The aim of this review article is...

Read More thumbnail image: Extraction approaches used for the determination of biologically active compounds cyclitols polyphenols and saponins isolated from plant material

Panel of serum protein biomarkers to grade the severity of traumatic brain injury

Published: Apr 25, 2018

Author(s): Raj Poovindran Anada, Kum Thong Wong, Jaime Jacqueline Jayapalan, Onn Haji Hashim, Dharmendra Ganesan

Journal: ELECTROPHORESIS

Abstract The Glasgow Coma Scale (GCS), which classifies patients into mild, moderate or severe traumatic brain injury (TBI), is a system used to prioritize treatment and prognosticate the severity of head injury. In this study, sera of patients with various stages of TBI, as well as control subjects, were analyzed to screen for proteins that may be used to complement the GCS system. By subjecting pooled serum samples to iTRAQ...

Read More thumbnail image: Panel of serum protein biomarkers to grade the severity of traumatic brain injury

Evaluation of six sample preparation procedures for qualitative and quantitative proteomics analysis of milk fat globule membrane

Published: Apr 25, 2018

Author(s): Yongxin Yang, Elizabeth Anderson, Sheng Zhang

Journal: ELECTROPHORESIS

Abstract Proteomic analysis of membrane proteins is challenged by the proteins solubility and detergent incompatibility with MS analysis. No single perfect protocol can be used to comprehensively characterize the proteome of membrane fraction. Here, we used cow milk fat globule membrane (MFGM) proteome analysis to assess six sample preparation procedures including one in‐gel and five in‐solution digestion approaches prior to...

Read More thumbnail image: Evaluation of six sample preparation procedures for qualitative and quantitative proteomics analysis of milk fat globule membrane

Profiling of small molecule metabolites and neurotransmitters in crustacean hemolymph and neuronal tissues using reversed‐phase LC‐MS/MS

Published: Apr 25, 2018

Author(s): Qinjingwen Cao, Chuanzi Ouyang, Xuefei Zhong, Lingjun Li

Journal: ELECTROPHORESIS

Abstract Crustaceans have been long used as model animals for neuromodulation studies because of their well‐defined neural circuitry. The identification of small molecule metabolites and signaling molecules in circulating fluids and neuronal tissues presents unique challenges due to their diverse structures, biological functions, and wide range of concentrations. LC combined with high resolution MS/MS is one of the most powerful...

Read More thumbnail image: Profiling of small molecule metabolites and neurotransmitters in crustacean hemolymph and neuronal tissues using reversedphase LCMSMS

A new method for microwave assisted ethanolic extraction of Mentha rotundifolia bioactive terpenoids

Published: Apr 25, 2018

Author(s): María Jesús García‐Sarrió, María Luz Sanz, Jesús Sanz, Azucena González‐Coloma, Ana Cristina Soria

Journal: ELECTROPHORESIS

Abstract A new microwave‐assisted extraction (MAE) method using ethanol as solvent has been optimized by means of a Box‐Behnken experimental design for the enhanced extraction of bioactive terpenoids from Mentha rotundifolia leaves; 100°C, 5 min, 1.125 g dry sample: 10 mL solvent and a single extraction cycle were selected as optimal conditions. Improved performance of MAE method in terms of extraction yield and/or...

Read More thumbnail image: A new method for microwave assisted ethanolic extraction of Mentha rotundifolia bioactive terpenoids

Separation of superparamagnetic magnetite nanoparticles by capillary zone electrophoresis using non‐complexing and complexing electrolyte anions and tetramethylammonium as dispersing additive

Published: Apr 25, 2018

Author(s): Monica N. Alves, Pavel N. Nesterenko, Brett Paull, Paul R. Haddad, Mirek Macka

Journal: ELECTROPHORESIS

Abstract Separations of bare superparamagnetic magnetite nanoparticles (BSPMNPs, approx. 11 nm diameter) was performed using non‐complexing (nitrate) and complexing (chloride, citrate and phosphate) electrolyte ions with additions of tetramethylammonium hydroxide (TMAOH), which is commonly applied to control the synthesis of stable iron oxides. The use of TMAOH as a background electrolyte (BGE) additive for capillary electrophoresis...

Read More thumbnail image: Separation of superparamagnetic magnetite nanoparticles by capillary zone electrophoresis using noncomplexing and complexing electrolyte anions and tetramethylammonium as dispersing additive

Determination of topiramate by capillary electrophoresis with capacitively‐coupled contactless conductivity detection: A powerful tool for therapeutic monitoring in epileptic patients

Published: Apr 25, 2018

Author(s): Aline Akemi Ishikawa, Rodrigo Moreira da Silva, Mauro Sérgio Ferreira Santos, Eric Tavares da Costa, Americo Ceiki Sakamoto, Emanuel Carrilho, Cristiane Masetto de Gaitani, Carlos D. Garcia

Journal: ELECTROPHORESIS

Abstract Topiramate (TPM) is the main antiepileptic drug used for the control of partial and generalized seizures in both adults and children. In association with clinical observations, the analysis of plasmatic concentration of TPM is of utmost importance for the individual adjustment of the administered dose to the patient. In the present work, a bioanalytical method was developed and validated for TPM analysis in plasma samples...

Read More thumbnail image: Determination of topiramate by capillary electrophoresis with capacitivelycoupled contactless conductivity detection A powerful tool for therapeutic monitoring in epileptic patients

Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach

Published: Apr 25, 2018

Author(s): Jian Xue, Riga Wu, Yajiao Pan, Shunxia Wang, Baowang Qu, Ying Qin, Yuequn Shi, Chuchu Zhang, Ran Li, Liyan Zhang, Cheng Zhou, Hongyu Sun

Journal: ELECTROPHORESIS

Abstract Massively parallel sequencing (MPS) technologies, also termed as next‐generation sequencing (NGS), are becoming increasingly popular in study of short tandem repeats (STR). However, current library preparation methods are usually based on ligation or two‐round PCR that requires more steps, making it time‐consuming (about 2 days), laborious and expensive. In this study, a 16‐plex STR typing system was designed with fusion...

Read More thumbnail image: Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach

Preparation and identification of oligosaccharides in lotus seeds and determination of their distribution in different parts of lotus

Published: Apr 25, 2018

Author(s): Lingxiao Chen, Dejun Hu, Xiao Liang, Jing Zhao, Shaoping Li

Journal: ELECTROPHORESIS

Abstract Three fractions (I–III) were separated from crude oligosaccharides of lotus seeds by fast protein liquid chromatography with final purity of 97.6, 96.3, and 96.8%, respectively. The fractions were identified as sucrose, raffinose, and stachyose by using TLC, HPLC with charged aerosol detector (CAD), LC‐MS, and methylation analysis. Subsequently sucrose and raffinose family oligosaccharides (RFOs) with degree of...

Read More thumbnail image: Preparation and identification of oligosaccharides in lotus seeds and determination of their distribution in different parts of lotus

Mineralogical investigations using XRD , XRF , and Raman spectroscopy in a combined approach

Published: Apr 25, 2018

Author(s): Maria Secchi, Marco Zanatta, Evgeny Borovin, Mauro Bortolotti, Arun Kumar, Marco Giarola, Andrea Sanson, Beate Orberger, Nicola Daldosso, Stefano Gialanella, Gino Mariotto, Maurizio Montagna, Luca Lutterotti

Journal: Journal of Raman Spectroscopy

Abstract The identification of mineralogical phases in drill cores is one of the most challenging tasks in the mining activity in view of an efficient metal extraction. This process requires the analytical characterization of large volumes of material to obtain a complete set of data in a minimum of time. None of the commonly used methods in mineralogical analyses, such as IR‐based techniques, X‐rays fluorescence, and hyperspectral...

Read More thumbnail image: Mineralogical investigations using XRD  XRF  and Raman spectroscopy in a combined approach

Unambiguous identification and characterization of a long‐term human metabolite of dehydrochloromethyltestosterone

Published: Apr 24, 2018

Author(s): Guro Forsdahl, Thomas Geisendorfer, Lorenz Göschl, Sandra Pfeffer, Peter Gärtner, Mario Thevis, Günter Gmeiner

Journal: Drug Testing and Analysis

Abstract In doping control analysis, the characterization of urinary steroid metabolites is of high interest for a targeted and long‐term detection of prohibited anabolic androgenic steroids (AAS). In this work, the structure of a long‐term metabolite of dehydrochloromethyltestosterone (DHCMT) was elucidated. Altogether, 8 possible metabolites with a 17α‐methyl‐17β‐hydroxymethyl – structures were synthesized and compared to a major...

Read More thumbnail image: Unambiguous identification and characterization of a longterm human metabolite of dehydrochloromethyltestosterone

An approach to shortening the timeframe between the emergence of new compounds on the drugs market and the availability of reference standards: The microscale syntheses of nitrazolam and clonazolam for use as reference materials, utilizing polymer‐supported reagents

Published: Apr 24, 2018

Author(s): Geraldine Dowling, Pierce V. Kavanagh, Hans‐Georg Eckhardt, Brendan Twamley, Gary Hessman, Gavin McLaughlin, John O'Brien, Simon D. Brandt

Journal: Drug Testing and Analysis

Abstract Nitrazolam and clonazolam are 2 designer benzodiazepines available from Internet retailers. There is growing evidence suggesting that such compounds have the potential to cause severe adverse events. Information about tolerability in humans is scarce but typically, low doses can be difficult to administer for users when handling bulk material. Variability of the active ingredient in tablet formulations can also be of a...

Read More thumbnail image: An approach to shortening the timeframe between the emergence of new compounds on the drugs market and the availability of reference standards The microscale syntheses of nitrazolam and clonazolam for use as reference materials utilizing polymersupported reagents

Driving under the influence of cocaine: Quantitative determination of basic drugs in oral fluid obtained during roadside controls and a controlled study with cocaine users

Published: Apr 24, 2018

Author(s): Vincent Di Fazio, Sarah M.R. Wille, Stefan W. Toennes, Janelle H.P. Wel, Johannes G. Ramaekers, Nele Samyn

Journal: Drug Testing and Analysis

Abstract Using the Belgian Drugs and Driving procedure, 36% of the cocaine‐positive oral fluid (OF) screening results were not confirmed in plasma. This study investigates the impact of the choice of screening devices and confirmation matrix on the detection of cocaine use. An ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method quantifying cocaine, benzoylecgonine (BZE), and other basic drugs in OF...

Read More thumbnail image: Driving under the influence of cocaine Quantitative determination of basic drugs in oral fluid obtained during roadside controls and a controlled study with cocaine users

Comparison of the MHC I Immunopeptidome Repertoire of B‐Cell Lymphoblasts Using Two Isolation Methods

Published: Apr 24, 2018

Author(s): Joël Lanoix, Chantal Durette, Mathieu Courcelles, Émilie Cossette, Simon Comtois‐Marotte, Marie‐Pierre Hardy, Caroline Côté, Claude Perreault, Pierre Thibault

Journal: PROTEOMICS

Abstract Significant technological advances in both affinity chromatography and mass spectrometry have facilitated the identification of peptides associated with the major histocompatibility complex class I (MHC I) molecules, and enabled a greater understanding of the dynamic nature of the immunopeptidome of normal and neoplastic cells. While the isolation of MHC I‐associated peptides (MIPs) typically used mild acid elution (MAE) or...

Read More thumbnail image: Comparison of the MHC I Immunopeptidome Repertoire of BCell Lymphoblasts Using Two Isolation Methods

Drug‐facilitated sexual assault (DFSA) involving 4‐methylethcathinone (4‐MEC), 3,4‐Methylenedioxypyrovalerone (MDPV), and doxylamine highlighted by hair analysis

Published: Apr 24, 2018

Author(s): Islam Amine Larabi, Marie Martin, Isabelle Etting, Pauline Penot, Nicolas Fabresse, Jean Claude Alvarez

Journal: Drug Testing and Analysis

Abstract Recently, the emergence of new psychoactive substances (NPS) has led to their wide use among clubbers and men who have sex with men (MSM) for their stimulant effects. However, their use in drug‐facilitated sexual assault (DFSA) has rarely been described. Herein we report a case of a 44‐year‐old man who was assaulted after a party. Due to late reporting of the offense, only hair (black) was sampled 15 days later and a...

Read More thumbnail image: Drugfacilitated sexual assault DFSA involving 4methylethcathinone 4MEC 34Methylenedioxypyrovalerone MDPV and doxylamine highlighted by hair analysis

Raman spectroscopy probing of redox states and mechanism of flavin coenzyme

Published: Apr 24, 2018

Author(s): Achut P. Silwal, H. Peter Lu

Journal: Journal of Raman Spectroscopy

Abstract Redox states of flavin mononucleotide (FMN) play important and regulating roles in living systems. To understand the involvement and contribution of FMN coenzyme in different biological processes, probing and characterizing of the associated FMN redox states using powerful experimental approaches are fundamental and crucial. In this study, we have generated a number of typical FMN redox states in Britton–Robinson buffer at...

Read More thumbnail image: Raman spectroscopy probing of redox states and mechanism of flavin coenzyme

Identification of erlotinib adsorption pattern onto silver nanoparticles: SERS studies

Published: Apr 24, 2018

Author(s): N. Piergies, M. Oćwieja, C. Paluszkiewicz, W.M. Kwiatek

Journal: Journal of Raman Spectroscopy

Abstract In this study, we present surface‐enhanced Raman spectroscopy (SERS) investigations for the erlotinib adsorbed onto silver nanoparticles (AgNPs). The observed spectral patterns, namely, changes in width and wavenumber of the SERS bands in comparison with these occurring in the corresponding Raman spectrum suggest how the investigated drug interacts with the applied metal nanoparticles. Moreover, the physicochemical...

Read More thumbnail image: Identification of erlotinib adsorption pattern onto silver nanoparticles SERS studies

Time‐domain measurement of pure rotational Raman collisional linewidths of ethane C 2 H 6

Published: Apr 24, 2018

Author(s): H. Zhang, F. Billard, O. Faucher, B. Lavorel

Journal: Journal of Raman Spectroscopy

Abstract The pure rotational Raman linewidths of the symmetric‐top molecule C2H6 are determined from a time‐resolved femtosecond experiment at room temperature and moderate pressure in a pump‐probe scheme. After excitation by a strong 100 fs‐duration nonresonant near infrared pump pulse, the molecular sample exhibits periodic post‐pulse alignment revivals that produce an anisotropic angular distribution of the molecular axis. Such molecular...

Read More thumbnail image: Timedomain measurement of pure rotational Raman collisional linewidths of ethane C 2 H 6

Comparative study of semiconductor TiO 2 and noble metal Ag substrates: The differences between chemical enhancement and electromagnetic enhancement in SERS

Published: Apr 24, 2018

Author(s): Xin Jiang, Yongliang Chen, Juan Du, Xiuling Li, Yu Shen, Ming Yang, Xiaoxia Han, Libin Yang, Bing Zhao

Journal: Journal of Raman Spectroscopy

Abstract The differences between electromagnetic enhancement and chemical enhancement in surface‐enhanced Raman scattering (SERS) and their deep insight are very important and valuable for the explanation of spectral behaviors and the practical application of SERS technique. In this work, the typical semiconductor substrate TiO2 nanoparticles and noble‐metal substrate Ag colloid were selected as two representative SERS‐active substrates to...

Read More thumbnail image: Comparative study of semiconductor TiO 2 and noble metal Ag substrates The differences between chemical enhancement and electromagnetic enhancement in SERS
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