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Towards polypotent natural products: The Derringer desirability approach and nonparametric ranking for multicriteria evaluation of essential oils

Published: Jun 19, 2018

Author(s): Filip Lj. Andrić

Journal: Journal of Chemometrics

Abstract Chemical complexity of natural products often results in their pharmacological polypotency. However, selecting a natural product with desirable activity profile is not a straightforward task, especially if optimization of one feature results in deterioration of other facets. Recently, in the field of multiobjective optimization, the sum of ranking differences (SRD) has emerged as a simple and...

Read More thumbnail image: Towards polypotent natural products The Derringer desirability approach and nonparametric ranking for multicriteria evaluation of essential oils

Determination of protein charge variants with (imaged) capillary isoelectric focusing and capillary zone electrophoresis

Published: Jun 19, 2018

Author(s): Julia Kahle, Hermann Wätzig

Journal: ELECTROPHORESIS

Abstract Biopharmaceuticals are increasingly present in pharmaceutical therapy. These highly complex molecules are still challenging for development and quality control. Charge heterogeneity is next to size heterogeneity, glycan analysis, or peptide mapping one main aspect of their thorough characterization. This review focuses on the charge‐based analysis of biopharmaceuticals through CE. An overview is given about the three...

Read More thumbnail image: Determination of protein charge variants with imaged capillary isoelectric focusing and capillary zone electrophoresis

Mass spectrometry is a multifaceted weapon to be used in the battle against Alzheimer's disease: Amyloid beta peptides and beyond

Published: Jun 15, 2018

Author(s): Giuseppe Grasso

Journal: Mass Spectrometry Reviews

Amyloid‐β peptide (Aβ) accumulation and aggregation have been considered for many years the main cause of Alzheimer's disease (AD), and therefore have been the principal target of investigation as well as of the proposed therapeutic approaches (Grasso [2011] Mass Spectrom Rev. 30: 347‐365). However, the amyloid cascade hypothesis, which considers Aβ accumulation the only causative agent of the disease, has proven to...

Read More thumbnail image: Mass spectrometry is a multifaceted weapon to be used in the battle against Alzheimers disease Amyloid beta peptides and beyond

Important considerations for the utilisation of methanolysis in steroid analysis

Published: Jun 14, 2018

Author(s): Marjaana Viljanto, Catarina Horro Pita, James Scarth, Christopher J. Walker, Andrew T. Kicman, Mark C. Parkin

Journal: Drug Testing and Analysis

Abstract The effective analysis of anabolic‐androgenic steroids in urine usually requires a suitable deconjugation method for the analysis of phase II metabolites such as sulphates and glucuronides. Acid hydrolysis using methanolysis is one adopted method of deconjugation that efficiently and rapidly cleaves both sulphates and glucuronides contemporaneously. The formation of artefactual by‐products is a known disadvantage of this...

Read More thumbnail image: Important considerations for the utilisation of methanolysis in steroid analysis

The effect of simulated space radiation on the N ‐glycosylation of human immunoglobulin G1

Published: Jun 14, 2018

Author(s): Mate Szarka, Szabolcs Szilasi, Boglarka Donczo, Daniel Sarkozy, Istvan Rajta, Andras Guttman

Journal: ELECTROPHORESIS

Abstract On a roundtrip to Mars, astronauts are expectedly exposed to an approximate amount of radiation that exceeds the lifetime limits on Earth. This elevated radiation dose is mainly due to Galactic Cosmic Rays and Solar Particle Events. Specific patterns of the N‐glycosylation of human Igs have already been associated with various ailments such as autoimmune diseases, malignant transformation, chronic inflammation, and ageing....

Read More thumbnail image: The effect of simulated space radiation on the N glycosylation of human immunoglobulin G1

Development of a fast CE method for high throughput screening of ecto‐5′‐nucleotidase inhibitors

Published: Jun 14, 2018

Author(s): Rabia Raza, Yu Bai, Huwei Liu

Journal: ELECTROPHORESIS

Abstract Overexpression of ecto‐5′‐nucleotidase is found to be linked to cancer progression and other diseases which makes screening of its inhibitors on demand. Unfortunately, there is scarcity of reported ecto‐5′‐nucleotidase inhibitors because of unavailability of simple, fast and efficient methods to study its inhibition. In this study, we have developed a very fast, reverse polarity capillary electrophoresis based method for...

Read More thumbnail image: Development of a fast CE method for high throughput screening of ecto5nucleotidase inhibitors

Gold surface plasmon crystal structure based‐on polystyrene template for biosensor application

Published: Jun 13, 2018

Author(s): Min‐Zhuo Cheng, Jing Zhang, Dequan Bao, Xiwei Huang

Journal: ELECTROPHORESIS

Abstract In this communication, we assembled ordered polystyrene (PS) microsphere array as a template with the drop‐coating method, and the oxygen plasma was used to etch the template to adjust the spacing between the PS microspheres. Nano‐triangular gold array and silver nano‐pyramid array were obtained by ion beam sputtering to deposit precious metal gold and silver. We observed the surface morphology of Au and Au/Ag composite...

Read More thumbnail image: Gold surface plasmon crystal structure basedon polystyrene template for biosensor application

Pressurized hot water extraction of bioactive compounds from artichoke by‐products

Published: Jun 13, 2018

Author(s): Imma Pagano, Anna Lisa Piccinelli, Rita Celano, Luca Campone, Patrizia Gazzerro, Mariateresa Russo, Luca Rastrelli

Journal: ELECTROPHORESIS

Abstract Artichoke by‐products are a suitable source of health‐promoting ingredients for the production of dietary supplements and food additives. A pressurized hot water extraction (PHWE) was developed to recover caffeoylquinic acids (CQAs) and flavone glycosides (FLs) from agro‐industrial artichoke by‐products. The main factors influencing PHWE efficiency and CQA isomerization (temperature, numbers of cycles, modifier, and...

Read More thumbnail image: Pressurized hot water extraction of bioactive compounds from artichoke byproducts

Structural and technological characterization of pectin extracted with sodium citrate and nitric acid from sunflower heads

Published: Jun 13, 2018

Author(s): Nerea Muñoz‐Almagro, Fabián Rico‐Rodriguez, Peter J. Wilde, Antonia Montilla, Mar Villamiel

Journal: ELECTROPHORESIS

Abstract An optimization of temperature, time, and extracting agent concentration of pectin extraction from sunflower heads using sodium citrate and nitric acid (SP‐SC and SP‐NA) was carried out. At optimal conditions, the yield of extraction with nitric acid (SPO‐NA) was twofold greater than the corresponding with sodium citrate (SPO‐SC) (14.3 versus 7.7%, respectively). Regarding pectin structure, the galacturonic acid (GalA)...

Read More thumbnail image: Structural and technological characterization of pectin extracted with sodium citrate and nitric acid from sunflower heads

Hollow mesoporous structured molecularly imprinted polymer as adsorbent in pipette‐tip solid‐phase extraction for the determination of antiretrovirals from plasma of HIV‐infected patients

Published: Jun 13, 2018

Author(s): Nathália Soares Simões, Hanna Leijoto Oliveira, Ricky Cássio Santos da Silva, Leila Suleimara Teixeira, Thaís Lorenna Souza Sales, Whocely Victor Castro, Maria José Nunes Paiva, Cristina Sanches, Keyller Bastos Borges

Journal: ELECTROPHORESIS

Abstract In this work a hollow mesoporous structured molecularly imprinted polymer was synthetized and used as adsorbent in pipette‐tip solid‐phase extraction for the determination of lamivudine (3TC), zidovudine (AZT) and efavirenz (EFZ) from plasma of human immunodeficiency virus (HIV) infected patients by high‐performance liquid chromatography (HPLC). All parameters that influence the recovery of the pipette tip based on hollow...

Read More thumbnail image: Hollow mesoporous structured molecularly imprinted polymer as adsorbent in pipettetip solidphase extraction for the determination of antiretrovirals from plasma of HIVinfected patients

Capillary zone electrophoresis for the determination of amodiaquine and three of its synthetic impurities in pharmaceutical formulations

Published: Jun 13, 2018

Author(s): Jean‐Pierre Mufusama, Ludwig Hoellein, Doris Feineis, Ulrike Holzgrabe, Gerhard Bringmann

Journal: ELECTROPHORESIS

Abstract A simple and robust CZE method was developed for the separation and quantification of the antimalarial compound amodiaquine as well as three of its synthetic impurities at a concentration equal to or lower than 0.5%. For capillary electrophoresis, a fused‐silica capillary, a background electrolyte of 100 mM sodium phosphate buffer at a pH value of 6.2, a voltage of +20 kV, and a detection wavelength of 220 nm...

Read More thumbnail image: Capillary zone electrophoresis for the determination of amodiaquine and three of its synthetic impurities in pharmaceutical formulations

An accurate bacterial DNA quantification assay for HTS library preparation of human biological samples

Published: Jun 13, 2018

Author(s): Sarah Seashols‐Williams, Raquel Green, Denise Wohlfahrt, Angela Brand, Antonio Limjuco Tan‐Torres, Francy Nogales, J Paul Brooks, Baneshwar Singh

Journal: ELECTROPHORESIS

Abstract Sequencing and classification of microbial taxa within forensically relevant biological fluids has the potential for applications in the forensic science and biomedical fields. The quantity of bacterial DNA from human samples is currently estimated based on quantity of total DNA isolated. This method can miscalculate bacterial DNA quantity due to the mixed nature of the sample, and consequently library preparation is often...

Read More thumbnail image: An accurate bacterial DNA quantification assay for HTS library preparation of human biological samples

Electromembrane extraction and capillary electrophoresis with capacitively coupled contactless conductivity detection: Multi‐extraction capabilities to analyses trace metals from saline samples

Published: Jun 13, 2018

Author(s): Macarena Silva, Carolina Mendiguchía, Carlos Moreno, Pavel Kubáň

Journal: ELECTROPHORESIS

Abstract Simultaneous electromembrane extraction (EME) of six trace metal cations (Cu2+, Zn2+, Co2+, Ni2+, Pb2+, Cd2+) from saline samples was investigated. CE with capacitively coupled contactless conductivity detection (C4D) was used to determine the metals in acceptor solutions due to its excellent compatibility with the minute volumes of acceptor solutions. Bis(2‐ethylhexyl)phosphate (DEHPA) was selected as a suitable nonselective modifier for EME...

Read More thumbnail image: Electromembrane extraction and capillary electrophoresis with capacitively coupled contactless conductivity detection Multiextraction capabilities to analyses trace metals from saline samples

Simultaneous quantification of fentanyl, sufentanil, cefazolin, doxapram and keto‐doxapram in plasma using liquid chromatography–tandem mass spectrometry

Published: Jun 12, 2018

Author(s): Robert B. Flint, Soma Bahmany, Bart C. H. Nagel, Birgit C. P. Koch

Journal: Biomedical Chromatography

Abstract A simple and specific UPLC–MS/MS method was developed and validated for simultaneous quantification of fentanyl, sufentanil, cefazolin, doxapram and its active metabolite keto‐doxapram. The internal standard was fentanyl‐d5 for all analytes. Chromatographic separation was achieved with a reversed‐phase Acquity UPLC HSS T3 column with a run‐time of only 5.0 min per injected sample. Gradient elution was performed with a...

Read More thumbnail image: Simultaneous quantification of fentanyl sufentanil cefazolin doxapram and ketodoxapram in plasma using liquid chromatographytandem mass spectrometry

Determining the effects of adulterants on drug detection via enzyme‐linked immunosorbent assay and adulterant tests strips

Published: Jun 11, 2018

Author(s): Bianca Olivieri, Mark Marić, Candice Bridge

Journal: Drug Testing and Analysis

Abstract The use of immunoassays for drug screening has increased due to their sensitivity towards target analytes, specifically the enzyme‐linked immunosorbent assay (ELISA) technique. Adulterant test strips are commonly used in conjunction with immunoassay tests to ensure the integrity of the specimen has not been altered prior to drug screening. This research focuses on determining which adulterants can produce a false negative...

Read More thumbnail image: Determining the effects of adulterants on drug detection via enzymelinked immunosorbent assay and adulterant tests strips

Simultaneous quantitation of rosuvastatin and ezetimibe in human plasma by LC–MS/MS: Pharmacokinetic study of fixed‐dose formulation and separate tablets

Published: Jun 11, 2018

Author(s): Abhaysingh Bhadoriya, Mallika Sanyal, Priyanka A. Shah, Pranav S. Shrivastav

Journal: Biomedical Chromatography

Abstract A simple, high‐throughput and highly sensitive liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) method has been developed for the simultaneous estimation of rosuvastatin and free ezetimibe. Liquid–liquid extraction was carried out using methyl‐tert butyl ether after prior acidification from 300 μL human plasma. The recovery for both the analytes and their deuterated internal standards (ISs) ranged from...

Read More thumbnail image: Simultaneous quantitation of rosuvastatin and ezetimibe in human plasma by LCMSMS Pharmacokinetic study of fixeddose formulation and separate tablets

Development of an HPLC‐DAD method for the determination of five alkaloids in Stephania yunnanensis Lo and in rat plasma after oral dose of Stephania yunnanensis Lo extracts

Published: Jun 11, 2018

Author(s): Aiyi Xin, Yaming Zhang, Yanxia Zhang, Duolong Di, Junxi Liu

Journal: Biomedical Chromatography

Abstract For the rational utilization and the quantitative quality control of the Stephania yunnanensis Lo, an HPLC‐DAD method was developed for the quantitative and simultaneous determination of five alkaloids in rat plasma (stepharine, sinomenine, palmatine, isocorydine and tetrahydropalmatine), which were the main active chemical constituents of this plant and belong to four kinds of isoquinoline‐type alkaloids (protoberberine, morphine,...

Read More thumbnail image: Development of an HPLCDAD method for the determination of five alkaloids in Stephania yunnanensis Lo and in rat plasma after oral dose of Stephania yunnanensis Lo extracts

Chiral separation of isoxanthohumol and 8‐prenylnaringenin in beer, hop pellets and hops by HPLC with chiral columns

Published: Jun 11, 2018

Author(s): Hyuga Moriya, Sohei Tanaka, Yukari Iida, Satomi Kitagawa, Sen‐ichi Aizawa, Atsushi Taga, Hiroyuki Terashima, Atsushi Yamamoto, Shuji Kodama

Journal: Biomedical Chromatography

Abstract Xanthohumol, isoxanthohumol, and 8‐prenylnaringenin in beer, hop and hop pellet samples were analyzed by HPLC using an InertSustain phenyl column and the mobile phase containing 40% methanol and 12% 2‐propanol. Fractions of isoxanthohumol and 8‐prenylnaringenin obtained by the above HPLC were separately collected. Isoxanthohumol and 8‐prenylnaringenin were enantioseparated by HPLC using a Chiralcel OD‐H column with a mobile...

Read More thumbnail image: Chiral separation of isoxanthohumol and 8prenylnaringenin in beer hop pellets and hops by HPLC with chiral columns

Inverse surface‐enhanced spatially offset Raman spectroscopy (SESORS) through a monkey skull

Published: Jun 11, 2018

Author(s): Ren A. Odion, Pietro Strobbia, Bridget M. Crawford, Tuan Vo‐Dinh

Journal: Journal of Raman Spectroscopy

Abstract The use of nanoparticles in nanomedicine has received increasing interest. However, in vivo detection of nanoparticles using optical techniques is still a formidable challenge. Detecting surface‐enhanced Raman scattering (SERS)‐labeled nanoparticles through thick tissue is crucial due to its large number of potential applications in the field of disease diagnostics and monitoring. As gold nanoparticles are becoming an...

Read More thumbnail image: Inverse surfaceenhanced spatially offset Raman spectroscopy SESORS through a monkey skull

DNA adducts: Formation, biological effects, and new biospecimens for mass spectrometric measurements in humans

Published: Jun 11, 2018

Author(s): Byeong Hwa Yun, Jingshu Guo, Medjda Bellamri, Robert J. Turesky

Journal: Mass Spectrometry Reviews

Hazardous chemicals in the environment and diet or their electrophilic metabolites can form adducts with genomic DNA, which can lead to mutations and the initiation of cancer. In addition, reactive intermediates can be generated in the body through oxidative stress and damage the genome. The identification and measurement of DNA adducts are required for understanding exposure and the causal role of a genotoxic...

Read More thumbnail image: DNA adducts Formation biological effects and new biospecimens for mass spectrometric measurements in humans
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