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Sequencing of mitochondrial genomes using the Precision ID mtDNA Whole Genome Panel

Published: Aug 6, 2018

Author(s): Vania Pereira, Antonio Longobardi, Claus Børsting

Journal: ELECTROPHORESIS

Abstract Massively parallel sequencing offers a fast and cost‐effective method for sequencing of the whole mtDNA genome. The Precision ID mtDNA Whole Genome Panel amplifies the entire mtDNA genome in two multiplex PCRs with 81 primer sets using the Ion AmpliSeq™ technology. In this study, the performance of the panel was evaluated by testing different amplification methods (two‐in‐one or conservative), the number of PCR cycles (21,...

Read More thumbnail image: Sequencing of mitochondrial genomes using the Precision ID mtDNA Whole Genome Panel

Investigation of carbon in the Allan Hills A77278 meteorite via 2D and 3D Raman microspectroscopy

Published: Aug 5, 2018

Author(s): Mehmet Yesiltas

Journal: Journal of Raman Spectroscopy

Abstract This paper reports the first Raman spectral and imaging data on the ordinary chondrite Allan Hills A77278 (ALHA77278) in two and three dimensions. Mineralogy in the ALHA77278 meteorite appears to be mainly silicates (olivine and pyroxene) with some carbonaceous material in the matrix. Metal phases are also present in the studied sample. Two‐dimensional Raman spectral distribution maps show the locations of these chemical...

Read More thumbnail image: Investigation of carbon in the Allan Hills A77278 meteorite via 2D and 3D Raman microspectroscopy

On the criteria for the discrimination of inkjet printer inks using micro‐ R aman spectroscopy

Published: Aug 5, 2018

Author(s): Patrick Buzzini, Carrie Polston, Madison Schackmuth

Journal: Journal of Raman Spectroscopy

Abstract Inkjet printers are ubiquitous devices in our society. Therefore, it is not surprising that they are used to commit different types of illicit activities such as threats or extortions by means of anonymous letters, frauds in the context of disputed contracts, alterations to or counterfeit of ID documents, or counterfeit of currency banknotes. This latter application is of primary interest in this study, where a method to...

Read More thumbnail image: On the criteria for the discrimination of inkjet printer inks using micro R aman spectroscopy

Sliding wear‐induced nano‐tribofilm formation from EP/AW agent on copper foil against ferroalloy counterparts under high load

Published: Aug 5, 2018

Author(s): Sang Xiong, Jianlin Sun

Journal: Surface and Interface Analysis

Sliding friction of copper foil surface, which was exposed to bis (6‐acetyl‐1,3,6,2 ‐dioxazaborocan‐2‐yl) methyl phosphate (NEPB) as extreme‐pressure/antiwear agent in base oil while rubbing by a ferroalloy disk under high load, was explored in a multi‐specimen test machine to investigate the wear mechanism of NEPB on thin film lubrication. The results reveal that NEPB reduces the friction coefficient to ~0.16 at normal load of...

Read More thumbnail image: Sliding wearinduced nanotribofilm formation from EPAW agent on copper foil against ferroalloy counterparts under high load

Recent progress in analytical capillary isotachophoresis

Published: Aug 3, 2018

Author(s): Zdena Malá, Petr Gebauer

Journal: ELECTROPHORESIS

Abstract This review brings a survey of studies on analytical ITP published since 2016 until the first quarter of 2018 and includes chapters about theory and principles, instrumentation and techniques, and analytical applications of ITP. It shows the position of analytical ITP among contemporary separation techniques, where particularly its unique concentrating capabilities keep the interest to include it into novel high‐sensitivity...

Read More thumbnail image: Recent progress in analytical capillary isotachophoresis

X‐ray‐induced reduction of a surfactant/polyoxotungstate hybrid compound

Published: Aug 2, 2018

Author(s): Aaron V. Colusso, Andrew McDonagh, Michael B. Cortie

Journal: Surface and Interface Analysis

We investigate the spontaneous reduction of a photochromic surfactant/polyoxotungstate hybrid during repeated X‐ray photoelectron spectroscopy (XPS) scans and show how this effect may confound attempts to use soft X‐rays to characterise materials of this nature. The W4f core‐level spectra revealed a progressive increase of W5+ and W4+ species at the expense of W6+ as irradiation time increased. The samples developed a blue colour attributed to the...

Read More thumbnail image: Xrayinduced reduction of a surfactantpolyoxotungstate hybrid compound

ZnO superhydrophobic coating via convenient spraying and its biofouling resistance

Published: Aug 2, 2018

Author(s): Changquan Li, Chan Xie, Junfei Ou, Mingshan Xue, Fajun Wang, Sheng Lei, Xinzuo Fang, Haoyu Zhou, Wen Li

Journal: Surface and Interface Analysis

The attachment of microbial biomass on solid surfaces, also referred to as biofouling, is a universal phenomenon that occurs in natural and engineering systems. However, traditional antibiofouling surfaces based on either the release of biocidal compounds or surface chemical/physical design have some drawbacks, such as the high cost, the complicated process, the low accuracy, and the limitation to achieve coatings over large area. Herein,...

Read More thumbnail image: ZnO superhydrophobic coating via convenient spraying and its biofouling resistance

Light‐induced self‐thermophoresis of Janus spheroidal nanoparticles

Published: Aug 2, 2018

Author(s): Touvia Miloh, Jacob Nagler

Journal: ELECTROPHORESIS

Abstract A theoretical framework is provided for determining the self‐thermophoretic velocity of a light irradiated spheroidal Janus nanoparticle consisting of symmetric dielectric and perfectly conducting semi‐spheroids. The analysis is based on solving the linearized Joule heating problem due to uniform laser irradiance and on explicitly determining the temperature fields inside and outside the particle. We employ the...

Read More thumbnail image: Lightinduced selfthermophoresis of Janus spheroidal nanoparticles

Contactless conductivity detection for analytical techniques: Developments from 2016 to 2018

Published: Aug 2, 2018

Author(s): Pavel Kubáň, Peter C. Hauser

Journal: ELECTROPHORESIS

Abstract The publications concerning capacitively coupled contactless conductivity detection for the 2‐year period from mid‐2016 to mid‐2018 are covered in this update to the earlier reviews of the series. Relatively few reports on fundamental investigations or new designs have appeared in the literature in this time interval, but the development of new applications with the detection method has continued strongly. Most often,...

Read More thumbnail image: Contactless conductivity detection for analytical techniques Developments from 2016 to 2018

Effectively controlled microfluidic trap for spatiotemporal analysis of the electrotaxis of Caenorhabditis elegans

Published: Aug 2, 2018

Author(s): Sunhee Yoon, Mingu Yeo, Hoon Kim, Tae‐Joon Jeon, Sun Min Kim

Journal: ELECTROPHORESIS

Abstract C. elegans is a popular model organism with a well‐developed neural network. Approximately 60% of the genes in C. elegans have genomic counterparts in humans, including those involved in building neural circuits. Therefore, we can extend the study of human neural network mechanisms to C. elegans which is easy to genetically manipulate. C. elegans shows behavioural responses to various external physical and chemical stimuli. Electrotaxis is one of...

Read More thumbnail image: Effectively controlled microfluidic trap for spatiotemporal analysis of the electrotaxis of Caenorhabditis elegans

DNA methylation assay based on pyrosequencing for determination of smoking status

Published: Aug 2, 2018

Author(s): Hussain Alghanim, Wensong Wu, Bruce McCord

Journal: ELECTROPHORESIS

Abstract The goal of this study was to utilize pyrosequencing to identify CpG sites indicative of tobacco smoking using DNA sequences surrounding ten frequently reported smoking‐related CpGs. Initially, six genetic loci were investigated including AHRR, 2q37, 6p21.33, GFI1, F2RL3, and MYO1G in order to detect novel CpG sites associated with tobacco smoking. The methylation data revealed a set of 23 consecutive CpG sites in blood...

Read More thumbnail image: DNA methylation assay based on pyrosequencing for determination of smoking status

Enantiomeric quality control of R ‐Tofisopam by HPLC using polysaccharide‐type chiral stationary phases in polar organic mode

Published: Aug 2, 2018

Author(s): Mohammadhassan Foroughbakhshfasaei, Zoltán‐István Szabó, Arash Mirzahosseini, Péter Horváth, Gergő Tóth

Journal: ELECTROPHORESIS

Abstract A novel, fast and economic chiral HPLC method was developed and validated for the resolution of the four isomers of tofisopam. The separation capacity of eleven different chiral columns: six polysaccharide‐type including three amylose‐based (Chiralpak AD, Chiralpak AD‐RH and Chiralpak AS) and three cellulose‐based (Chiralcel OD, Chiralcel OJ and Lux Cellulose‐4); three cyclodextrin‐ (Quest‐BC, Quest‐C2 and Quest‐CM) and two...

Read More thumbnail image: Enantiomeric quality control of R Tofisopam by HPLC using polysaccharidetype chiral stationary phases in polar organic mode

Use of Endoglycosidase H as a diagnostic tool for MAN1B1‐CDG patients

Published: Aug 2, 2018

Author(s): Sandrine Duvet, Dounia Mouajjah, Romain Péanne, Gert Matthijs, Kimiyo Raymond, Jaak Jaeken, Eva Morava, François Foulquier

Journal: ELECTROPHORESIS

Abstract Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities. With some 33 reported patients, MAN1B1‐CDG belongs to the more frequent causes of CDG‐II. MAN1B1 encodes an α1,2‐mannosidase that removes the terminal mannose residue from the middle branch. Several methods have been proposed to characterize the glycosylation changes. In MAN1B1‐CDG, the abnormal accumulating...

Read More thumbnail image: Use of Endoglycosidase H as a diagnostic tool for MAN1B1CDG patients

Nanopore label‐free detection of single‐nucleotide deletion in Baxα/BaxΔ2

Published: Aug 2, 2018

Author(s): Xiaohan Chen, Liang Wang, Golbarg M Roozbahani, Youwen Zhang, Jialing Xiang, Xiyun Guan

Journal: ELECTROPHORESIS

Abstract Baxα, a key tumor suppressor gene, will not be expressed correctly as a result of single nucleotide mutation in its microsatellite region; Instead, BaxΔ2, an isoform of Baxα, is often produced. In addition, lack of the exon 2 due to an alternative splicing, BaxΔ2 has the same sequence as Baxα except single base deletion from eight continuous guanines (G8) to G7. Most of the currently available methods for Bax∆2 detection...

Read More thumbnail image: Nanopore labelfree detection of singlenucleotide deletion in BaxBax2

Analyzing histological material to determine ajmaline and other drugs using high‐performance liquid chromatography/tandem mass spectrometry

Published: Aug 1, 2018

Author(s): Katarina Henning, Marek Dziadosz, Michael Klintschar, Birgit Piep, Jörg Teske

Journal: Drug Testing and Analysis

Read More thumbnail image: Analyzing histological material to determine ajmaline and other drugs using highperformance liquid chromatographytandem mass spectrometry

Simultaneous electrokinetic and hydrodynamic injection and sequential stacking featuring sweeping for signal amplification following MEKC during the analysis of rapamycin (sirolimus) in serum samples

Published: Aug 1, 2018

Author(s): Ilona Olędzka, Piotr Kowalski, Alina Plenis, Natalia Miękus, Niels Grabow, Thomas Eickner, Tomasz Bączek

Journal: ELECTROPHORESIS

Abstract Simultaneous electrokinetic and hydrodynamic injection of rapamycin (sirolimus) with off‐line and online sample preconcentration techniques and using MEKC has been studied. Compared to conventional hydrodynamic injection, a 168‐fold improvement in the signal was obtained with a combination of simultaneous electrokinetic and hydrodynamic injectionand field enhanced sample injection in conjunction with a sweeping technique...

Read More thumbnail image: Simultaneous electrokinetic and hydrodynamic injection and sequential stacking featuring sweeping for signal amplification following MEKC during the analysis of rapamycin sirolimus in serum samples

Volumetric assessment of spontaneous mechanical activities by simultaneous multi‐slice MRI techniques with correlation to muscle fiber orientation

Published: Aug 1, 2018

Author(s): Martin Schwartz, Petros Martirosian, Günter Steidle, Michael Erb, Alto Stemmer, Bin Yang, Fritz Schick

Journal: NMR in Biomedicine

The purpose of this work was assessment of volumetric characteristics of spontaneous mechanical activities in musculature (SMAMs) by diffusion‐weighted simultaneous multi‐slice (DW‐SMS) imaging and spatial correlation to anatomical structure, as revealed by fusion to fiber tractographic information derived from diffusion‐tensor imaging (DTI). The feasibility of using DW‐SMS to image spontaneous events in human...

Read More thumbnail image: Volumetric assessment of spontaneous mechanical activities by simultaneous multislice MRI techniques with correlation to muscle fiber orientation

Detection of microscopic traces of explosive residues on textile fabrics by Raman spectroscopy

Published: Aug 1, 2018

Author(s): Diogo Videira‐Quintela, Félix Zapata, Carmen García‐Ruiz

Journal: Journal of Raman Spectroscopy

Abstract Direct or cross‐contamination of explosive residues on clothing is of high occurrence when handling explosive materials, leading to physically trapped particles between the fibres. In this work, the detection and identification of trace amounts of organic explosives, inorganic explosives, and oxidizing salts trapped between the fibres of dyed and undyed synthetic and natural textile clothing fabrics were studied using two...

Read More thumbnail image: Detection of microscopic traces of explosive residues on textile fabrics by Raman spectroscopy

Effect of nitrogen doping on the photocatalytic activity and hydrophobic property of rutile TiO2 nanorods array

Published: Aug 1, 2018

Author(s): Peerawas Kongsong, Abdelhafed Taleb, Mahamasuhaimi Masae, Ausmee Jeenarong, Pariyaphan Hansud, Sakorn Khumruean

Journal: Surface and Interface Analysis

Pure titanium dioxide (TiO2) and nitrogen (N)–doped TiO2 nanorods array were prepared using polished Ti substrates and hydrothermal synthesis method. Prepared nanorods films were characterized by different techniques including X‐ray diffractometer (XRD), field‐emission scanning electron microscope, Fourier‐transform infrared spectroscopy, and XPS. The photocatalytic activities of both prepared films were evaluated by their capacity to degrade ...

Read More thumbnail image: Effect of nitrogen doping on the photocatalytic activity and hydrophobic property of rutile TiO2 nanorods array

A microchip electrophoretic assay for DNA methyltransferase activity based on methylation‐sensitive endonuclease DpnⅡ

Published: Aug 1, 2018

Author(s): Yating Zhang, Yan Zhang, Luqi Zhu, Pingang He, Qingjiang Wang

Journal: ELECTROPHORESIS

Abstract DNA methylation is a significant epigenetic modification and the methods for the detection of DNA methyltransferase (MTase) activity are important due to aberrant methylation closely related to the occurrence of cancer. In this study, a simple and rapid microchip electrophoresis (ME) coupled with LED‐induced fluorescence (LEDIF) method was presented for the detection of Dam MTase activity. This strategy was based on...

Read More thumbnail image: A microchip electrophoretic assay for DNA methyltransferase activity based on methylationsensitive endonuclease Dpn
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