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Dielectrophoretic trapping of single leukemic cells using the conventional and compact optical measurement systems

Published: Feb 5, 2019

Author(s): Hamideh Sharifi Noghabi, Mandy Soo, Avid Khamenehfar, Paul C.H. Li

Journal: ELECTROPHORESIS

Abstract Here, we report a microfluidic same‐single‐cell analysis to study the inhibition of multidrug resistance due to drug efflux on single leukemic cells. Drug efflux inhibition was investigated in the microfluidic chip using two different fluorescence detection systems, namely, a compact single‐cell bioanalyzer and the conventional optical detection system constructed from an inverted microscope and a microphotometer. More importantly, a compact signal generator was used to conduct...

Read More thumbnail image: Dielectrophoretic trapping of single leukemic cells using the conventional and compact optical measurement systems

Detection of RNA‐protein interactions using a highly sensitive non‐radioactive electrophoretic mobility shift assay

Published: Feb 5, 2019

Author(s): Gerasimos Daras, Anastasios Alatzas, Dikran Tsitsekian, Dimitris Templalexis, Stamatis Rigas, Polydefkis Hatzopoulos

Journal: ELECTROPHORESIS

Abstract Electrophoretic mobility shift assay (EMSA) is a sensitive technique useful in the identification and characterization of protein interactors with nucleic acids. This assay provides an efficient method to study DNA or RNA binding proteins and to identify nucleic acid substrates. The specific interaction plays important roles in many biological processes such as transcription, translation, splicing, and global gene expression. In this article, we have modified the EMSA technique and...

Read More thumbnail image: Detection of RNAprotein interactions using a highly sensitive nonradioactive electrophoretic mobility shift assay

Sebacic acid as corrosion inhibitor for hot‐dip galvanized (HDG) steel in 0.1 M NaCl

Published: Feb 4, 2019

Author(s): Michele Fedel, Mireille Poelman, Marjorie Olivier, Flavio Deflorian

Journal: Surface and Interface Analysis

The potential of sebacic acid as a corrosion inhibitor for hot‐dip galvanized steel in 0.1 M NaCl solution has been investigated. Different concentrations of the organic acid have been tested in order to understand the inhibition mechanism of the compound. The electrochemical test revealed a competitive mechanism between the dissolution of the metal in the acidified solution and the inhibition provided by the dicarboxylic species. The formation of a whitish layer consisting of zinc...

Read More thumbnail image: Sebacic acid as corrosion inhibitor for hotdip galvanized HDG steel in 0_1M NaCl

Molecular hydrogen‐induced nucleation of hydrogenated silicon nanocrystals at low temperature

Published: Feb 4, 2019

Author(s): Larbi Filali, Yamina Brahmi, Jamal Dine Sib, Fatiha Kail, Yahya Bouizem, Djamel Benlakehal, Kacem Zellama, Ahmed Bouhekka, Aissa Kebab, Larbi Chahed

Journal: Surface and Interface Analysis

Highly crystallized hydrogenated silicon layers were obtained via the treatment of hydrogenated polymorphous silicon films in a molecular hydrogen ambient. This contrasts other postdeposition studies that obtained nanocrystalline silicon films but necessitated either a plasma activation or high‐temperature annealing. The structure of the samples was analyzed by Raman spectroscopy to determine the crystallite volume fraction, which was found to increase up to 80% within 1 hour of treatment....

Read More thumbnail image: Molecular hydrogeninduced nucleation of hydrogenated silicon nanocrystals at low temperature

Ti (IV) attached‐phosphonic acid functionalized capillary monolith as a stationary phase for in‐syringe‐type fast and robust enrichment of phosphopeptides

Published: Feb 4, 2019

Author(s): Kouroush Salimi, Çiğdem Kip, Ömür Çelikbıçak, Duygu Deniz Usta, Aslı Pınar, Bekir Salih, Ali Tuncel

Journal: Biomedical Chromatography

Abstract In this study, poly(vinylphosphonic acid‐co‐ethylene dimethacrylate), poly(VPA‐co‐EDMA) capillary monolith was synthesized as a starting material for obtaining a stationary phase for microscale enrichment of phosphopeptides. The chelation of active phosphonate groups with Ti (IV) ions gave a macroporous monolithic column with a mean pore size of 5.4 μm. The phosphopeptides from different sources were enriched on Ti (IV)‐attached poly(VPA‐co‐EDMA) monolith using a syringe‐pump. The...

Read More thumbnail image: Ti IV attachedphosphonic acid functionalized capillary monolith as a stationary phase for insyringetype fast and robust enrichment of phosphopeptides

Peptide nucleic acid and amino acid modified peptide nucleic acid analysis by capillary zone electrophoresis

Published: Feb 4, 2019

Author(s): Xiaoqian Wang, Linsen Li, Rizwan Ullah Khan, Feng Qu

Journal: ELECTROPHORESIS

Abstract A rapid, high resolution, and low sample consumption CZE method is developed for peptide nucleic acid (PNA) analysis for the first time. 30% v/v acetonitrile in PNA sample and 20% v/v acetonitrile in 50 mM borax‐boric acid (pH 8.7) as BGE were employed after optimization. The calibration curves were linear for PNA concentration ranging from 1 to 50 μmol/L. LOD and LOQ of PNA were 0.2 and 1.0 μmol/L, respectively. Since the commercially available reagent gives rise to huge PNA peak and...

Read More thumbnail image: Peptide nucleic acid and amino acid modified peptide nucleic acid analysis by capillary zone electrophoresis

Auger electron spectroscopic analysis of corrosion products formed on A3003 aluminum alloy in model fresh water with different Zn2+ concentration

Published: Feb 4, 2019

Author(s): Kyohei Otani, Md. Saiful Islam, Masatoshi Sakairi, Akira Kaneko

Journal: Surface and Interface Analysis

The corrosion morphology and composition of corrosion products of A3003 formed in model fresh water with different Zn2+ concentrations were investigated by immersion tests combined with surface observations and analysis using an Auger electron spectroscope (AES). The cross‐sectional AES observations showed that the thickness of the corrosion product layer formed on A3003 decreases with increases in the Zn2+ concentration of the model fresh water. A cross‐sectional AES point analysis suggested...

Read More thumbnail image: Auger electron spectroscopic analysis of corrosion products formed on A3003 aluminum alloy in model fresh water with different Zn2 concentration

Click regulation of cyclodextrin primary face for the preparation of novel chiral stationary phases

Published: Feb 1, 2019

Author(s): Xuan Jin, Xiaoxuan Li, Yong Wang

Journal: ELECTROPHORESIS

Abstract In this study, a series of novel CD chiral stationary phases were fabricated by immobilization of mono‐6A‐deoxy‐N3‐cyclodextrin onto silica surfaces followed by click regulation of CD primary face with 4‐pentynoic acid (acidic moiety), 2‐propynylamine (alkaline moiety) and L‐propargylglycine (chiral amino acid moiety), respectively. Enantioseparations of various kinds of racemates including dansyl‐amino acids, chiral lactides and diketones were conducted in reversed phase modes on...

Read More thumbnail image: Click regulation of cyclodextrin primary face for the preparation of novel chiral stationary phases

Recommendations for reporting ion mobility Mass Spectrometry measurements

Published: Feb 1, 2019

Author(s): Valérie Gabelica, Alexandre A. Shvartsburg, Carlos Afonso, Perdita Barran, Justin L.P. Benesch, Christian Bleiholder, Michael T. Bowers, Aivett Bilbao, Matthew F. Bush, J. Larry Campbell, Iain D.G. Campuzano, Tim Causon, Brian H. Clowers, Colin S. Creaser, Edwin De Pauw, Johann Far, Francisco Fernandez‐Lima, John C. Fjeldsted, Kevin Giles, Michael Groessl, Christopher J. Hogan, Stephan Hann, Hugh I. Kim, Ruwan T. Kurulugama, Jody C. May, John A. McLean, Kevin Pagel, Keith Richardson, Mark E. Ridgeway, Frédéric Rosu, Frank Sobott, Konstantinos Thalassinos, Stephen J. Valentine, Thomas Wyttenbach

Journal: Mass Spectrometry Reviews

Here we present a guide to ion mobility mass spectrometry experiments, which covers both linear and nonlinear methods: what is measured, how the measurements are done, and how to report the results, including the uncertainties of mobility and collision cross section values. The guide aims to clarify some possibly confusing concepts, and the reporting recommendations should help researchers, authors and reviewers to contribute comprehensive reports, so that the ion mobility data can be reused...

Read More thumbnail image: Recommendations for reporting ion mobility Mass Spectrometry measurements

Atmospheric aerosol analysis at the PIXE–RBS beamline in the University of Jordan Van de Graaff accelerator (JUVAC)

Published: Jan 31, 2019

Author(s): Hanan Sa'adeh, Massimo Chiari

Journal: X-Ray Spectrometry

The University of Jordan Van de Graaff accelerator (JUVAC) was devoted many years ago to promote scientific research through the installation of several beamlines based on nuclear techniques. It constitutes the first and unique, till now, ion beam analysis facility in Jordan. Among these techniques, the JUVAC has recently installed, with the financial and technical aid of the International Atomic Energy Agency, a combination of particle‐induced X‐ray emission (PIXE) and Rutherford...

Read More thumbnail image: Atmospheric aerosol analysis at the PIXERBS beamline in the University of Jordan Van de Graaff accelerator JUVAC

Pressurized liquid extraction of cyclitols and sugars: optimization of extraction parameters and selective separation

Published: Jan 31, 2019

Author(s): Hossam Al‐Suod, Ileana‐Andreea Ratiu, Ryszard Górecki, Bogusław Buszewski

Journal: Journal of Separation Science

Abstract Cyclitols and sugars were obtained as a mixture from Medicago sativa L., in a comparative study by using maceration, and pressurized liquid extraction, as a modern and green extraction techniques. The influence of extraction parameters including: extraction temperature, time and number of cycles on the content of sugars and cyclitols was investigated based on response surface methodology. The highest total amount of sugars and cyclitols (62.27 ± 2.30 and 50.35 ± 0.77 mg/g of dry...

Read More thumbnail image: Pressurized liquid extraction of cyclitols and sugars optimization of extraction parameters and selective separation

Highlighting the uniqueness in dielectrophoretic enrichment of circulating tumor cells

Published: Jan 31, 2019

Author(s): Florina S. Iliescu, Wen Jing Sim, Hossein Heidari, Daniel P. Poenar, Jianmin Miao, Hayden K. Taylor, Ciprian Iliescu

Journal: ELECTROPHORESIS

Abstract Circulating tumor cells (CTCs) play an essential role in the metastasis of tumors, and thus can serve as a valuable prognostic factor for malignant diseases. As a result, the ability to isolate and characterize CTCs is essential. This review underlines the potential of dielectrophoresis for CTCs enrichment. It begins by summarizing the key performance parameters and challenges of CTCs isolation using microfluidics. The two main categories of CTCs enrichment—affinity‐based and...

Read More thumbnail image: Highlighting the uniqueness in dielectrophoretic enrichment of circulating tumor cells

The degree of substitution affects the enantioselectivity of sulfobutylether‐β‐cyclodextrin chiral stationary phases

Published: Jan 31, 2019

Author(s): Denisa Folprechtová, Květa Kalíková, Petr Kozlík, Eva Tesařová

Journal: ELECTROPHORESIS

Abstract Three chiral stationary phases were prepared by dynamic coating of sulfobutylether‐β‐cyclodextrin (SBE‐β‐CD) with different degrees of substitution, onto strong anion‐exchange stationary phases. The enantioselective potential and stability of newly prepared chiral stationary phases were examined using a set of structurally different chiral analytes. Measurements were performed in RP‐HPLC. Mobile phases consisted of methanol/formic acid, pH 2.10, and methanol/10 mM ammonium acetate...

Read More thumbnail image: The degree of substitution affects the enantioselectivity of sulfobutylethercyclodextrin chiral stationary phases

Study of the mainstream cigarette smoke aerosols by Fourier transform ion cyclotron resonance mass spectrometry coupled to laser/desorption and electrospray ionization – Additional insights on the heteroaromatic components

Published: Jan 31, 2019

Author(s): Adama Kamissoko, Vincent Carré, Sébastien Schramm, Frédéric Aubriet

Journal: Rapid Communications in Mass Spectrometry

Rationale The chemical composition of the particulate phase of cigarette smoke inhaled by the active smoker is still poorly known in spite of its importance from a health point of view. A non‐targeted approach is applied to cigarette smoke particles collected on a quartz filter to obtain an as complete as possible description of this complex mixture. Methods A home‐made smoking machine including devices for volatile organic compounds (VOCs) and particle sampling was used. The validation of the...

Read More thumbnail image: Study of the mainstream cigarette smoke aerosols by Fourier transform ion cyclotron resonance mass spectrometry coupled to laserdesorption and electrospray ionization  Additional insights on the heteroaromatic components

Matrix‐assisted ionization Fourier transform mass spectrometry for the analysis of lipids

Published: Jan 31, 2019

Author(s): Rohana Liyanage, Jennifer Gidden, Charles L. Wilkins, Jackson O. Lay

Journal: Rapid Communications in Mass Spectrometry

Rationale Assessing the utility of vacuum matrix‐assisted ionization (MAI) for the direct and rapid analysis of lipids in complex samples with emphasis on bacterial taxonomy. Methods Matrix‐assisted ionization Fourier transform mass spectrometry (MAI‐FTMS) was used to characterize polar and non‐polar lipids in mixtures. Results For non‐polar lipid triacylglycerols (TAGs), MAI‐FTMS produced lipid‐specific ions for eight different edible oils and allowed these oils to be identified based on...

Read More thumbnail image: Matrixassisted ionization Fourier transform mass spectrometry for the analysis of lipids

Importance of collision cross section measurements by ion mobility mass spectrometry in structural biology

Published: Jan 31, 2019

Author(s): Tara Pukala

Journal: Rapid Communications in Mass Spectrometry

The field of ion mobility mass spectrometry (IM‐MS) has developed rapidly in recent decades, with new fundamental advances underpinning innovative applications. This has been particularly noticeable in the field of biomacromolecular structure determination and structural biology, with pioneering studies revealing new structural insight for complex protein assemblies which control biological function. This perspective offers a review of recent developments in IM‐MS which have enabled expanding...

Read More thumbnail image: Importance of collision cross section measurements by ion mobility mass spectrometry in structural biology

Exosomes Derived from Human Primary and Metastatic Colorectal Cancer Cells Contribute to Functional Heterogeneity of Activated Fibroblasts by Reprogramming Their Proteome

Published: Jan 31, 2019

Author(s): Alin Rai, David W. Greening, Maoshan Chen, Rong Xu, Hong Ji, Richard J. Simpson

Journal: PROTEOMICS

Abstract Cancer‐associated fibroblasts (CAFs) are a heterogeneous population of activated fibroblasts that constitute a dominant cellular component of the tumor microenvironment (TME) performing distinct functions. Here, the role of tumor‐derived exosomes (Exos) in activating quiescent fibroblasts into distinct functional subtypes is investigated. Proteomic profiling and functional dissection reveal that early‐ (SW480) and late‐stage (SW620) colorectal cancer (CRC) cell‐derived Exos both...

Read More thumbnail image: Exosomes Derived from Human Primary and Metastatic Colorectal Cancer Cells Contribute to Functional Heterogeneity of Activated Fibroblasts by Reprogramming Their Proteome

Optimization of parabolic cell for gas Raman analysis

Published: Jan 30, 2019

Author(s): Anlan Yu, Duluo Zuo, Xinbing Wang

Journal: Journal of Raman Spectroscopy

Abstract Raman spectroscopy is a widely practicable technique in gas analysis, but the application in simultaneous detection of multiple‐trace gas is still a challenge for its weak signal level. To extend the application of Raman gas analysis to detection of trace gas constituents, a closed parabolic sample cell proposed in our previous work is optimized by optical analysis and experimental verification. The optical analysis shows that for a parabolic reflector with rim diameter 6p (p,...

Read More thumbnail image: Optimization of parabolic cell for gas Raman analysis

Detection and quantification of heavy metal elements in gallstones using X‐ray fluorescence spectrometry

Published: Jan 30, 2019

Author(s): Brij Bir Singh Jaswal, Pradeep Kumar Rai, Tejbir Singh, Vassilia Zorba, Vivek Kumar Singh

Journal: X-Ray Spectrometry

Gallbladder cancer, which is associated with the heavy metal toxicity, is one of the most dangerous malignancies. High occurrence of gallstones in the gallbladder is a well‐recognized risk factor for gallbladder cancer. Accumulation of heavy and toxic metals in the biliary system is one of the main causes of gallstone formation and leads to gallbladder cancer if it remains untreated. In this work, we investigate heavy and toxic metals in gallstones to identify their association as potential...

Read More thumbnail image: Detection and quantification of heavy metal elements in gallstones using Xray fluorescence spectrometry

Analysis of precious metals from the tomb of the “Lady of Cao” by X‐ray microtomography and digital radiography

Published: Jan 30, 2019

Author(s): Soraia R. Azeredo, Roberto Cesareo, Régulo F. Jordan, Arabel Fernandez, Giovanni E. Gigante, Angel Bustamante, Ricardo T. Lopes

Journal: X-Ray Spectrometry

Investigative analyses of metallic objects from the tomb of a queen of the Moche civilization, who lived around 100 to 300 AD in the northern coast of current Peru, known as Lady of Cao, have been carried out by researchers in the last 4 years using various destructive techniques. Many of these objects found inside the tomb are jewelry produced using silver, gilded copper, copper, and gold sheets. The application of X‐ray microtomography (μCT) and X‐ray digital radiography techniques using a...

Read More thumbnail image: Analysis of precious metals from the tomb of the Lady of Cao by Xray microtomography and digital radiography
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