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Phenotypic Analysis of Organoids by Proteomics

Published: Sep 7, 2017

Author(s): Alexis Gonneaud, Claude Asselin, François Boudreau, François‐Michel Boisvert

Journal: PROTEOMICS

Abstract The development of 3D cell cultures into self‐organizing organ‐like structures named organoids provides a model that better reflects in vivo organ physiology and their functional properties. Organoids have been established from several organs, such as the intestine, prostate, brain, liver, kidney and pancreas. With recent advances in high‐throughput and ‐omics profiling technologies, it is now possible to study the...

Read More thumbnail image: Phenotypic Analysis of Organoids by Proteomics

Recent developments in open tubular capillary electrochromatography from 2016 to 2017

Published: Sep 6, 2017

Author(s): Faustino M. Tarongoy, Paul R. Haddad, Joselito P. Quirino

Journal: ELECTROPHORESIS

Abstract Interest in open‐tubular capillary electrochromatography (OT‐CEC) continues to thrive because of the inherent advantage of OT‐CEC combining the high efficiency of capillary electrophoresis and the high selectivity of high performance liquid chromatography. For the period 2016 to 2017, novel materials have been developed as first‐time stationary phases for OT‐CEC and are grouped in this review as polymer‐based materials,...

Read More thumbnail image: Recent developments in open tubular capillary electrochromatography from 2016 to 2017

Uniform‐sized neurosphere‐mediated motoneuron differentiation in microwell arrays

Published: Sep 6, 2017

Author(s): Jong Min Lee, Joo Yoon Moon, Mohammed R. Shaker, Woong Sun, Bong Geun Chung

Journal: ELECTROPHORESIS

We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform‐sized neurospheres harvested from microwell arrays were replated into...

Read More thumbnail image: Uniformsized neurospheremediated motoneuron differentiation in microwell arrays

Comparison of antimicrobial peptide purification via free‐flow electrophoresis and gel filtration chromatography

Published: Sep 6, 2017

Author(s): Zhi‐Jun Xia, Zhen Liu, Fan‐Zhi Kong, Liu‐Yin Fan, Hua Xiao, Cheng‐Xi Cao

Journal: ELECTROPHORESIS

Abstract Antimicrobial peptides (AMPs) are usually small and cationic biomolecules with broad‐spectrum antimicrobial activities against pathogens. Purifying them from complex samples is essential to study their physiochemical properties. In this work, free‐flow zone electrophoresis (FFZE) was utilized to purify AMPs from yeast fermentation broth. Meanwhile, gel filtration chromatography (GFC) was conducted for comparison. The...

Read More thumbnail image: Comparison of antimicrobial peptide purification via freeflow electrophoresis and gel filtration chromatography

Quantification of free fatty acids in human stratum corneum using tandem mass spectrometry and surrogate analyte approach

Published: Sep 5, 2017

Author(s): Irena Dapic, Renata Kobetic, Lidija Brkljacic, Sanja Kezic, Ivone Jakasa

Journal: Biomedical Chromatography

Abstract The free fatty acids (FFAs) are one of the major components of the lipids in the stratum corneum (SC), the uppermost layer of the skin. Relative composition of FFAs has been proposed as a biomarker of the skin barrier status in patients with atopic dermatitis (AD). Here, we developed an LC‐ESI‐MS/MS method for simultaneous quantification of a range of FFAs with long and very long chain length in the SC collected by adhesive...

Read More thumbnail image: Quantification of free fatty acids in human stratum corneum using tandem mass spectrometry and surrogate analyte approach

Sulfonated poly(styrene‐divinylbenzene) modified with amines and the application for pipette‐tip solid‐phase extraction of carbendazim in apples

Published: Sep 5, 2017

Author(s): Yuxin Ma, Lingling Liu, Weiyang Tang, Tao Zhu

Journal: Journal of Separation Science

Abstract Sulfonated poly(styrene‐divinylbenzene) modified with five kinds of amine functional groups was applied to the determination of carbendazim in apple samples with a pipette‐tip solid‐phase extraction method. The structures of the polymers were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. Five different modifications of the solid‐phase extraction...

Read More thumbnail image: Sulfonated polystyrenedivinylbenzene modified with amines and the application for pipettetip solidphase extraction of carbendazim in apples

Screening bioactive quality control markers of QiShenYiQi dripping pills based on the relationship between the ultra‐high performance liquid chromatography fingerprint and vascular protective activity

Published: Sep 5, 2017

Author(s): Limeng Zhuo, Jingjing Peng, Yunli Zhao, Dongxiang Li, Xiuman Xie, Ling Tong, Zhiguo Yu

Journal: Journal of Separation Science

Abstract Traditional Chinese medicine consists of complex phytochemical constituents. Selecting appropriate analytical markers of traditional Chinese medicine is a critical step in quality control. Currently, the combination of fingerprinting and efficacy evaluation is considered as a useful method for screening active ingredients in complex mixtures. This study was designed to develop an orthogonal partial least squares model for...

Read More thumbnail image: Screening bioactive quality control markers of QiShenYiQi dripping pills based on the relationship between the ultrahigh performance liquid chromatography fingerprint and vascular protective activity

Potential impact of the sewer system on the applicability of alcohol and tobacco biomarkers in wastewater‐based epidemiology

Published: Sep 5, 2017

Author(s): Andrew P.W. Banks, Foon Yin Lai, Jochen F. Mueller, Guangming Jiang, Steve Carter, Phong K. Thai

Journal: Drug Testing and Analysis

Abstract Understanding the actual consumption of alcohol and tobacco in the population is important for forming public health policy. For this purpose, wastewater‐based epidemiology has been applied as a complementary method to estimate the overall alcohol and tobacco consumption in different communities. However, the stability of their consumption biomarkers – ethyl sulfate, ethyl glucuronide, cotinine, and trans‐3′‐hydroxycotinine...

Read More thumbnail image: Potential impact of the sewer system on the applicability of alcohol and tobacco biomarkers in wastewaterbased epidemiology

Confirmation of recent heroin abuse: Accepting the challenge

Published: Sep 5, 2017

Author(s): Alexandra Maas, Burkhard Madea, Cornelius Hess

Journal: Drug Testing and Analysis

Abstract Confirmation or exclusion of recent heroin consumption is still one of the major challenges for forensic and clinical toxicologists. A great variety of biomarkers is available for heroin abuse confirmation, including various opium alkaloids (eg, morphine, codeine), street heroin impurities (eg, 6‐acetylcodeine [6‐AC], noscapine, papaverine) as well as associated metabolites (eg, 6‐monoacetylmorphine [6‐MAM], morphine...

Read More thumbnail image: Confirmation of recent heroin abuse Accepting the challenge

Multicomponent determination of traditional Chinese medicine preparation yin‐zhi‐huang injection by LC–MS/MS for screening of its potential bioactive candidates using HepaRG cells

Published: Sep 4, 2017

Author(s): Zhi Rao, Fan Zhang, Xiao‐Yi Zhang, Guo‐Qiang Zhang, Yan‐Rong Ma, Yan Zhou, Hong‐Yan Qin, Xin‐An Wu, Yu‐Hui Wei

Journal: Biomedical Chromatography

Abstract Yin‐zhi‐huang (YZH) injection is an injectable multiherbal prescription derived from the ancient Chinese medicine formula of Yin‐chen‐hao‐tang, which is widely used in the clinic for the treatment of jaundice and chronic liver diseases. To date, the systematic study of the components in this multiherbal prescription still lacks suitable analytical methods that are able to simultaneously detect a broad array of components at...

Read More thumbnail image: Multicomponent determination of traditional Chinese medicine preparation yinzhihuang injection by LCMSMS for screening of its potential bioactive candidates using HepaRG cells

Numerical analysis of a dielectrophoresis field‐flow fractionation device for the separation of multiple cell types

Published: Sep 4, 2017

Author(s): Amir Shamloo, Ali Kamali

Journal: Journal of Separation Science

In this study, a dielectrophoresis field‐flow fractionation device was analyzed using a numerical simulation method and the behaviors of a set of different cells were investigated. By reducing the alternating current frequency of the electrodes from the value used in the original setup configuration and increasing the number of exit channels, total discrimination in cell trajectories and subsequent separation of four cell types were...

Read More thumbnail image: Numerical analysis of a dielectrophoresis fieldflow fractionation device for the separation of multiple cell types

Fast analysis of quaternary ammonium pesticides in food and beverages using cation‐exchange chromatography coupled with isotope‐dilution high‐resolution mass spectrometry

Published: Sep 4, 2017

Author(s): Tiziana Nardin, Chiara Barnaba, Franco Abballe, Gianmaria Trenti, Mario Malacarne, Roberto Larcher

Journal: Journal of Separation Science

Abstract A fast separation based on cation‐exchange liquid chromatography coupled with high‐resolution mass spectrometry is proposed for simultaneous determination of chlormequat, difenzoquat, diquat, mepiquat and paraquat in several food and beverage commodities. Solid samples were extracted using a mixture of water/methanol/formic acid (69.6:30:0.4, v/v/v), while liquid samples were ten times diluted with the same solution....

Read More thumbnail image: Fast analysis of quaternary ammonium pesticides in food and beverages using cationexchange chromatography coupled with isotopedilution highresolution mass spectrometry

Proteomics in Cell Division

Published: Sep 4, 2017

Author(s): Zeynep Cansu Üretmen Kagıalı, Aydanur Şentürk, Nazlı Ezgi Özkan Küçük, Mohammad Haroon Qureshi, Nurhan Özlü

Journal: PROTEOMICS

Abstract Cell division requires a coordinated action of the cell cycle machinery, cytoskeletal elements, chromosomes, and membranes. Cell division studies have greatly benefitted from the mass spectrometry (MS)‐based proteomic approaches for probing the biochemistry of highly dynamic complexes and their coordination with each other as a cell progresses into division. In this review, the authors first summarize a wide‐range of...

Read More thumbnail image: Proteomics in Cell Division

Chemical UPLC‐ESI‐MS/MS profiling of aconitum alkaloids and their metabolites in rat plasma and urine after oral administration of Aconitum carmichaelii Debx. Root extract

Published: Sep 3, 2017

Author(s): Mingjie Zhang, Manman Wang, Jiajia Liang, Yongqing Wen, Zhili Xiong

Journal: Biomedical Chromatography

Abstract In this paper, an ultra high performance liquid chromatography tandem mass spectrometric (UPLC‐ESI‐MS/MS) method in positive ion mode was established to systematically identify and to compare the major aconitum alkaloids and their metabolites in rat plasma and urine after oral administration of Fuzi extract. A total twenty‐nine components including twenty‐five C19‐diterpenoid alkaloids and four C20‐diterpenoid alkaloids...

Read More thumbnail image: Chemical UPLCESIMSMS profiling of aconitum alkaloids and their metabolites in rat plasma and urine after oral administration of Aconitum carmichaelii Debx_ Root extract

Rapid automatic creation of monodisperse emulsion droplets by microfluidic device with degassed PDMS slab as a detachable suction actuator

Published: Sep 1, 2017

Author(s): Yuki Murata, Yuta Nakashoji, Masaki Kondo, Yugo Tanaka, Masahiko Hashimoto

Journal: ELECTROPHORESIS

We previously developed a technique that enabled automatic creation of monodisperse water‐in‐oil droplets with the use of an air‐evacuated PDMS microfluidic device. Although the device generated droplets over a long‐time period, the production rate was slow (∼10 droplets per second). In the current study, we aimed to improve this rate, using the same fluid pumping principle described in our previous work, by remodeling our device...

Read More thumbnail image: Rapid automatic creation of monodisperse emulsion droplets by microfluidic device with degassed PDMS slab as a detachable suction actuator

Synthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis

Published: Sep 1, 2017

Author(s): Ederson Paulo Xavier Guilherme, Jocilane Pereira Oliveira, Lorendane Millena Carvalho, Igor Viana Brandi, Sérgio Henrique Sousa Santos, Gleidson Giordano Pinto Carvalho, Junio Cota, Bruna Mara Aparecida de Carvalho

Journal: ELECTROPHORESIS

A bioreactor was built by means of immobilizing alpha‐amylase from Aspergillus oryzae by encapsulation, through cryopolymerization of acrylamide monomers for the continuous starch hydrolysis. The starch hydrolysis was evaluated regarding pH, the concentration of immobilized amylase on cryogel, the concentration of starch solution and temperature. The maximum value for starch hydrolysis was achieved at pH 5.0, concentration of immobilized enzyme...

Read More thumbnail image: Synthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis

Advances in the analysis of “less‐conventional” human body fluids: An overview of the CE‐ and HPLC‐MS applications in the years 2015–2017

Published: Sep 1, 2017

Author(s): Monica Di Venere, Simona Viglio, Maddalena Cagnone, Anna Bardoni, Roberta Salvini, Paolo Iadarola

Journal: ELECTROPHORESIS

Aim of this article is to focus the attention of the reader on the application of CE/MS and LC/MS to the analysis of human body fluids not currently used for the diagnosis of disorders and, for this reason, catalogued as “less/nonconventional” fluids, that is, tears, nasal secretions, cerumen, bronchoalveolar lavage fluid, sputum, exhaled breath condensate, nipple aspirate, breast milk, amniotic fluid, bile, seminal plasma, liposuction...

Read More thumbnail image: Advances in the analysis of lessconventional human body fluids An overview of the CE and HPLCMS applications in the years 20152017

Mutation rates in 21 autosomal short tandem repeat loci in a population from Goiás, Brazil

Published: Sep 1, 2017

Author(s): Thaís Cidália Vieira, Marc Alexandre Duarte Gigonzac, Ricardo Goulart Rodovalho, Luana Morais Cavalcanti, Lysa Bernardes Minasi, Flávia Melo Rodrigues, Aparecido Divino da Cruz

Journal: ELECTROPHORESIS

The appearance of new mutations in polymorphic markers plays a central role in a range of genetic applications, including dating phylogenetic events, informing disease studies, and evaluating forensic evidence. The present study estimated the mutation rates of 21 autosomal STR loci in a population from Central Brazil. We studied 15 046 paternity cases from Goiás, Brazil from August 2012 to February 2015. We identified 262 mutations...

Read More thumbnail image: Mutation rates in 21 autosomal short tandem repeat loci in a population from Goias Brazil

Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

Published: Aug 31, 2017

Author(s): Hong Wan, Bo Zhang, Xiao‐Lin Bai, Yan Zhao, Meng‐Wei Xiao, Xun Liao

Journal: Journal of Separation Science

A new, rapid, green, and cost‐effective magnetic solid‐phase extraction of ochratoxin A from red wine samples was developed using polydopamine‐coated magnetic multi‐walled carbon nanotubes as the absorbent. The polydopamine‐coated magnetic multi‐walled carbon nanotubes were fabricated with magnetic multi‐walled carbon nanotubes and dopamine by an in situ oxidative self‐polymerization approach. Transmission electron microscopy, dynamic...

Read More thumbnail image: Extraction of ochratoxin A in red wine with dopaminecoated magnetic multiwalled carbon nanotubes

Magnetic porous carbon derived from Co‐doped metal–organic frameworks for the magnetic solid‐phase extraction of endocrine disrupting chemicals

Published: Aug 31, 2017

Author(s): Lin Hao, Jiayi Wei, Ruixue Zheng, Chun Wang, Qiuhua Wu, Zhi Wang

Journal: Journal of Separation Science

Metal–organic frameworks‐5 (MOF‐5) was explored as a template to prepare porous carbon due to its high surface area, large pore volume, and permanent nanoscale porosity. Magnetic porous carbon, Co@MOF‐5‐C, was fabricated by the one‐step direct carbonization of Co‐doped MOF‐5. After carbonization, the magnetic cobalt nanoparticles are well dispersed in the porous carbon matrix, and Co@MOF‐5‐C displays strong magnetism (with the saturation...

Read More thumbnail image: Magnetic porous carbon derived from Codoped metalorganic frameworks for the magnetic solidphase extraction of endocrine disrupting chemicals
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