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Formation of Heterotypic Amyloids: α‐Synuclein in Co‐Aggregation

Published: Oct 10, 2018

Author(s): Karishma Bhasne, Samrat Mukhopadhyay

Journal: PROTEOMICS

Abstract Protein misfolding resulting in the formation of ordered amyloid aggregates is associated with a number of devastating human diseases. Intrinsically disordered proteins (IDPs) do not autonomously fold up into a unique stable conformation and remain as an ensemble of rapidly fluctuating conformers. Many IDPs are prone to convert into the β‐rich amyloid state. One such amyloidogenic IDP is α‐synuclein that is involved in...

Read More thumbnail image: Formation of Heterotypic Amyloids Synuclein in CoAggregation

Trapping DNA with a high throughput microfluidic device

Published: Oct 10, 2018

Author(s): Ryan J. Montes, Jason E. Butler, Anthony J. C. Ladd

Journal: ELECTROPHORESIS

Abstract Long strands of DNA can be trapped and concentrated near the inlet of a microfluidic channel by applying a pressure gradient and an opposing electric field. The mechanism for trapping involves a migration of DNA perpendicular to both the fluid flow and the electric field. Migration leads to a highly nonuniform distribution of DNA within a cross section of the channel, with the bulk of the DNA concentrated in a thin (10 μm)...

Read More thumbnail image: Trapping DNA with a high throughput microfluidic device

Facile synthesis of titanium(IV) ion‐immobilized poly‐glycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate for highly specific enrichment of intact phosphoproteins

Published: Oct 10, 2018

Author(s): Hao Wang, Zhixin Tian

Journal: Journal of Separation Science

Abstract In this study, a facile synthesis of the titanium(IV) ion‐immobilized poly‐glycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate was developed for efficient enrichment of intact phosphoproteins. The titanium(IV) ion‐immobilized microparticles had higher saturated adsorption capacity for phosphoproteins (1217.6 mg/g for β‐casein) than nonphosphoproteins (97.1 mg/g for...

Read More thumbnail image: Facile synthesis of titaniumIV ionimmobilized polyglycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate for highly specific enrichment of intact phosphoproteins

Resolving power in liquid chromatography: A trade‐off between efficiency and analysis time

Published: Oct 10, 2018

Author(s): José Luís Dores‐Sousa, Jelle Vos, Sebastiaan Eeltink

Journal: Journal of Separation Science

Abstract This review describes chromatographic dispersion and different plate‐height models frequently used to assess the chromatographic performance of ultra‐high‐pressure liquid chromatography column technology. Furthermore, different performance indices, including the resolution, the separation impedance, and kinetic plots are discussed allowing to quantify and visualize the resolving power in liquid chromatography. The...

Read More thumbnail image: Resolving power in liquid chromatography A tradeoff between efficiency and analysis time

Determination of siloxanes in water samples employing graphene oxide/Fe 3 O 4 nanocomposite as sorbent for magnetic solid‐phase extraction prior to GC–MS

Published: Oct 10, 2018

Author(s): Luciana Costa dos Reis, Lorena Vidal, Antonio Canals

Journal: Journal of Separation Science

Abstract A new, fast, simple, and environmentally friendly analytical method has been developed to determine six siloxanes in water samples: octamethyltrisiloxane, octamethylcyclotetrasiloxane, decamethyltetrasiloxane, decamethylcyclopentasiloxane, dodecamethylpentasiloxane and dodecamethylcyclohexasiloxane. The analytical method consists of magnetic solid‐phase extraction employing graphene oxide/Fe3O4 as sorbent for the separation and...

Read More thumbnail image: Determination of siloxanes in water samples employing graphene oxideFe 3 O 4 nanocomposite as sorbent for magnetic solidphase extraction prior to GCMS

An environmentally friendly method for the enantioseparation and determination of benalaxyl in tobacco and soil by ultra‐performance convergence chromatography with tandem mass spectrometry

Published: Oct 10, 2018

Author(s): Fei Yang, Gangling Tang, Zhonghao Li, Ziyan Fan, Ying Wang, Shanshan Liu, Zhaoyang Bian, Huimin Deng

Journal: Journal of Separation Science

Abstract For the purpose of chiral separation and determination of benalaxyl enantiomers in tobacco and soil, we developed a rapid, green, and sensitive method using ultra‐performance convergence chromatography with tandem mass spectrometry. The samples were extracted and purified by the quick, easy, cheap, effective, rugged, and safe method before injection. The baseline separation was obtained on a chiral column in 5 min with...

Read More thumbnail image: An environmentally friendly method for the enantioseparation and determination of benalaxyl in tobacco and soil by ultraperformance convergence chromatography with tandem mass spectrometry

Rapid isolation and characterization of crocins, picrocrocin, and crocetin from saffron using centrifugal partition chromatography and LC–MS

Published: Oct 10, 2018

Author(s): Evangelia Karkoula, Apostolis Angelis, Nikolaos‐Stavros Koulakiotis, Evangelos Gikas, Maria Halabalaki, Anthony Tsarbopoulos, Alexios‐Leandros Skaltsounis

Journal: Journal of Separation Science

Abstract This study demonstrates a simple method for one‐step isolation of the main secondary metabolites of a hydroalcoholic extract of Crocus sativus stigmas (saffron) using step‐gradient centrifugal partition chromatography. The analysis was performed in dual and elution−extrusion mode, using five biphasic systems of the solvents heptane/ethyl acetate/butanol/ethanol/water in ratios of 4:10:0:4:10, 1:13:0:4:10, 1:12:1:4:10,...

Read More thumbnail image: Rapid isolation and characterization of crocins picrocrocin and crocetin from saffron using centrifugal partition chromatography and LCMS

Sum frequency generation study of immobilized 3‐aminopropyltriethoxysilane self‐assembled layer on Si(111) substrates

Published: Oct 10, 2018

Author(s): Thi Thu Hien Khuat, Lin Liang, Trong Tue Phan, Goro Mizutani, Harvey N. Rutt

Journal: Surface and Interface Analysis

Distribution and orientation of 3‐aminopropyltriethoxysilane (APTES) on a sensing surface play critical role on biosensor, but have not been fully investigated. In this work, the quality of APTES SAM immobilized on the Si(111) substrate was studied by observing sum frequency generation (SFG) response. The polarization, azimuthal angle, and position‐dependent SFG spectra of APTES coverage were obtained. In ppp and psp‐SFG spectra, the CH2...

Read More thumbnail image: Sum frequency generation study of immobilized 3aminopropyltriethoxysilane selfassembled layer on Si111 substrates

Identification of Moonlighting Proteins in Genomes Using Text Mining Techniques

Published: Oct 10, 2018

Author(s): Aashish Jain, Hareesh Gali, Daisuke Kihara

Journal: PROTEOMICS

Abstract Moonlighting proteins is an emerging concept for considering protein functions, which indicate proteins with two or more independent and distinct functions. An increasing number of moonlighting proteins have been reported in the past years; however, a systematic study of the topic has been hindered because the secondary functions of proteins are usually found serendipitously by experiments. Toward systematic identification...

Read More thumbnail image: Identification of Moonlighting Proteins in Genomes Using Text Mining Techniques

Metabolic fate of the new synthetic cannabinoid 7’N‐5F‐ADB in rat, human, and pooled human S9 studied by means of hyphenated high‐resolution mass spectrometry

Published: Oct 9, 2018

Author(s): Lilian H.J. Richter, Hans H. Maurer, Markus R. Meyer

Journal: Drug Testing and Analysis

Abstract New psychoactive substances (NPS) are an important issue in clinical/forensic toxicology. 7’N‐5F‐ADB, a synthetic cannabinoid derived from 5F‐ADB, appeared recently on the market. Up to now, no data about its mass spectral fragmentation pattern, metabolism, and thus suitable targets for toxicological urine screenings have been available. Therefore, the aim of this study was to elucidate the metabolic fate of 7’N‐5F‐ADB in...

Read More thumbnail image: Metabolic fate of the new synthetic cannabinoid 7N5FADB in rat human and pooled human S9 studied by means of hyphenated highresolution mass spectrometry

Development and validation of LC–MS/MS method for quantification of bictegravir in human plasma and its application to an intracellular uptake study

Published: Oct 9, 2018

Author(s): Pavan Kumar Prathipati, Subhra Mandal, Christopher J. Destache

Journal: Biomedical Chromatography

Abstract A sensitive liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed and validated for quantification of bictegravir in human plasma. A small volume of only 50 μL aliquot of plasma was precipitated with acetonitrile containing an internal standard (IS). Chromatographic separation was performed on a Kinetex EVO C18 column, 50 × 3.0 mm, 5 μm using an isocratic mobile phase containing 80:20 acetonitrile–water with...

Read More thumbnail image: Development and validation of LCMSMS method for quantification of bictegravir in human plasma and its application to an intracellular uptake study

Quantitative determination of six steroid alkaloids by sensitive hydrophilic interaction liquid chromatography electrospray ionization mass spectrometry and its application to pharmacokinetic study in rats

Published: Oct 9, 2018

Author(s): Jianbo Chen, Lixue Chen, Meijia Li, Yinshi Sun

Journal: Biomedical Chromatography

Abstract In this study, a sensitive hydrophilic interaction liquid chromatography (HILIC) method was developed to determine pseudojervine (PJV), veratrosine (VTS), jervine (JV), veratramine (VTM), veramarine (VA) and veratroylzygadenine (VTG) in rat plasma. Separations were carried out using LC–MS/MS with a Chrom Matrix HP amide column (5 m, 10 cm × 3.0 mm i.d.). The mobile phases were (A) 0.01 mm formic acid and (B) acetonitrile....

Read More thumbnail image: Quantitative determination of six steroid alkaloids by sensitive hydrophilic interaction liquid chromatography electrospray ionization mass spectrometry and its application to pharmacokinetic study in rats

Advances in mass spectrometry‐based glycomics

Published: Oct 9, 2018

Author(s): Xue Dong, Yifan Huang, Byeong Gwan Cho, Jieqiang Zhong, Sakshi Gautam, Wenjing Peng, Seth D. Williamson, Alireza Banazadeh, Katya Y. Torres‐Ulloa, Yehia Mechref

Journal: ELECTROPHORESIS

Abstract The diversification of the chemical properties and biological functions of proteins is attained through posttranslational modifications, such as glycosylation. Glycans, which are covalently attached to proteins, play a vital role in cell activities. The microheterogeneity and complexity of glycan structures associated with proteins make comprehensive glycomic analysis challenging. However, recent advancements in mass...

Read More thumbnail image: Advances in mass spectrometrybased glycomics

Advances in mass spectrometry‐based glycoproteomics

Published: Oct 9, 2018

Author(s): Aiying Yu, Jingfu Zhao, Wenjing Peng, Alireza Banazadeh, Seth D. Williamson, Mona Goli, Yifan Huang, Yehia Mechref

Journal: ELECTROPHORESIS

Abstract Protein glycosylation, an important PTM, plays an essential role in a wide range of biological processes such as immune response, intercellular signaling, inflammation, and host–pathogen interaction. Aberrant glycosylation has been correlated with various diseases. However, studying protein glycosylation remains challenging because of low abundance, microheterogeneities of glycosylation sites, and poor ionization efficiency...

Read More thumbnail image: Advances in mass spectrometrybased glycoproteomics

Enantiomers separation by capillary electrochromatography using polysaccharide‐based stationary phases

Published: Oct 9, 2018

Author(s): Giovanni D’ Orazio, María Asensio‐Ramos, Chiara Fanali

Journal: Journal of Separation Science

Abstract The separation of chiral compounds is an interesting and important topic of research because these compounds are involved in some biological processes, fundamentally in human health. Among the various application fields where enantiomers are remarkable, drug analysis has to be considered. Most of the drugs contain enantiomers and very often one of the two isomers could be pharmacologically more active or even dangerous....

Read More thumbnail image: Enantiomers separation by capillary electrochromatography using polysaccharidebased stationary phases

The New Face of the Lipid Droplet: Lipid Droplet Proteins

Published: Oct 9, 2018

Author(s): Congyan Zhang, Pingsheng Liu

Journal: PROTEOMICS

Abstract The lipid droplet (LD) is an organelle with vital functions found in nearly all organisms. LD proteomic research has provided fundamentally important insights into this organelle's functions. The review provides a summary of LD proteomic studies conducted across diverse organisms and cell and tissue types. The accumulated proteomic data are reviewed for evidence of a protein targeting mechanism for the organelle. The...

Read More thumbnail image: The New Face of the Lipid Droplet Lipid Droplet Proteins

Determination of nitenpyram and 6‐chloronicotinic acid in environmental samples by ion chromatography coupled with online photochemically induced fluorescence detector

Published: Oct 9, 2018

Author(s): Nadeem Muhammad, Yun Zhang, Weixia Li, Yong‐Gang Zhao, Amjad Ali, Qamar Subhani, Tariq Mahmud, Junwei Liu, Hairong Cui, Yan Zhu

Journal: Journal of Separation Science

Abstract A simple, cost‐effective, sensitive, and quick method for the determination of nitenpyram and its metabolite 6‐chloronicotinic acid in environmental samples was developed by coupling an ion chromatograph with a fluorescence detector and a post‐column photochemical reactor. This developed analytical method involved a rapid sample extraction by modified and miniaturized quick, easy, cheap, effective, rugged, and safe method...

Read More thumbnail image: Determination of nitenpyram and 6chloronicotinic acid in environmental samples by ion chromatography coupled with online photochemically induced fluorescence detector

PHOTOIONIZATION AND PHOTOFRAGMENTATION IN MASS SPECTROMETRY WITH VISIBLE AND UV LASERS

Published: Oct 9, 2018

Author(s): Frank Gunzer, Sascha Krüger, Jürgen Grotemeyer

Journal: Mass Spectrometry Reviews

Ever since the introduction of laser technology to the field of mass spectrometry, several disciplines evolved providing solutions to challenging scientific and analytical tasks in research and industry. Among these are techniques involving multiphoton ionization such as Resonance‐Enhanced Multiphoton Ionization (REMPI, R2PI) and Mass‐Analyzed Threshold Ionization (MATI) spectroscopy, a variant of Zero Kinetic Energy...

Read More thumbnail image: PHOTOIONIZATION AND PHOTOFRAGMENTATION IN MASS SPECTROMETRY WITH VISIBLE AND UV LASERS

A method for improvement of mass resolution and isotope accuracy for laser ablation time‐of‐flight mass spectrometers

Published: Oct 9, 2018

Author(s): Reto Wiesendanger, Marek Tulej, Valentine Grimaudo, Alena Cedeño‐López, Rustam Lukmanov, Andreas Riedo, Peter Wurz

Journal: Journal of Chemometrics

Abstract Accumulation of spectra is a common approach for improvement of the signal‐to‐noise ratio (SNR) in mass spectrometry. However, severe degradation of the overall spectrum can occur if some individual mass spectra, affected by peak broadening, are included in the accumulation process. In this contribution, we discuss potential sources and effects of spectral distortions by using examples from mass spectra acquired by our...

Read More thumbnail image: A method for improvement of mass resolution and isotope accuracy for laser ablation timeofflight mass spectrometers

Performance of methods that separate common and distinct variation in multiple data blocks

Published: Oct 9, 2018

Author(s): Ingrid Måge, Age K. Smilde, Frans M. Kloet

Journal: Journal of Chemometrics

Abstract In many areas of science, multiple sets of data are collected from the samples. Such data sets can be analyzed by multiblock (or data fusion) methods. The aim is usually to get a holistic understanding of the system or better prediction of some response. Lately, several scientific groups have developed methods for separating common and distinct variation between multiple data blocks. Although the objective is the same, the strategies...

Read More thumbnail image: Performance of methods that separate common and distinct variation in multiple data blocks
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