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Journal Highlight: Routine quantification of 54 allergens in fragrances using comprehensive GCxGC-QMS with dual parallel secondary columns

Date: Jan 5, 2018

Author: separationsNOW

Scientists in Switzerland and Italy have combined two-dimensional gas chromatography with both a quadrupole mass spectrometer and a flame ionization detector to produce a novel method for analyzing allergens in fragrances.

Read More thumbnail image: Journal Highlight Routine quantification of 54 allergens in fragrances using comprehensive GCxGC-QMS with dual parallel secondary columns

Journal Highlight: Characterization of a robust serine protease from Bacillus subtilis K-1

Date: Jan 5, 2018

Author: separationsNOW

Researchers in India used ion-exchange chromatography and size-exclusion chromatography to extract and purify a robust protease from the bacterium Bacillus subtilis.

Read More thumbnail image: Journal Highlight Characterization of a robust serine protease from Bacillus subtilis K-1

Heart failure: HDL protein signals

Date: Jan 3, 2018

Author: Steve Down

The proteome of high-density lipoprotein is different for survivors of heart failure and those who died, say an international team of researchers who found that a group of 12 proteins gave strong predictive capabilities.

Read More thumbnail image: Heart failure HDL protein signals

Journal Highlight: How many proteins can be identified in a 2-DE gel spot within an analysis of a complex human cancer tissue proteome?

Date: Jan 1, 2018

Author: separationsNOW

Three different mass spectrometric techniques have been compared for the identification of proteins within a single 2-DE gel spot, confirming the necessity of isotopic labeling in large-scale quantification of different protein species in a proteome.

Read More thumbnail image: Journal Highlight How many proteins can be identified in a 2-DE gel spot within an analysis of a complex human cancer tissue proteome

Chemometrics gets to the root of TCM UHPLC data

Date: Jan 1, 2018

Author: Ryan De Vooght-Johnson

Morinda root, or ba ji tian, is commonly used in traditional Chinese medicine for a variety of conditions, but there is a lack of analytical methods that give an overall picture of the active compounds present. Researchers from the Institute of Medicinal Plant Development in China have developed a method using UHPLC to assay 11 bioactive compounds in morinda root, classifying samples by means of hierarchical cluster analysis, principal component analysis and partial least-squares discriminant analysis.

Read More thumbnail image: Chemometrics gets to the root of TCM UHPLC data

Tobacco cheats caught by smoking gun of VOCs

Date: Jan 1, 2018

Author: Ryan De Vooght-Johnson

There is a need to be able to distinguish smokers from non-smokers by testing chemicals in their breath, but it is hard to find appropriate marker compounds. Scientists from the Institute of Microelectronics and Microsystems in Italy, have developed a new method of examining the volatile organic compounds (VOCs) in smokers’ breath using GC-MS and statistical tests.

Read More thumbnail image: Tobacco cheats caught by smoking gun of VOCs

LC-MS can show the way to measure hypoglycin A

Date: Jan 1, 2018

Author: Ryan De Vooght-Johnson

Atypical myopathy is a potentially fatal horse disease caused by eating toxic plant material, such as sycamore seeds. Researchers from Jena University Hospital and the FZMB Veterinary Clinic in Bad Langensalza in Germany have developed a new UHPLC-MS/MS method to quantify the relevant toxins (hypoglycin A and MPCA-carnitine) in horse serum.

Read More thumbnail image: LC-MS can show the way to measure hypoglycin A

Journal Highlight: Biochemical characterization and structural analysis of trypsin from Plodia interpunctella midgut

Date: Dec 25, 2017

Author: separationsNOW

Scientists in Iran have used ion exchange chromatography to characterize trypsin from the Indian mealmoth (Plodia interpunctella).

Read More thumbnail image: Journal Highlight Biochemical characterization and structural analysis of trypsin from Plodia interpunctella midgut

Journal Highlight: A platinized stainless steel fiber with in-situ coated polyaniline/polypyrrole/graphene oxide nanocomposite sorbent for headspace SPME of aliphatic aldehydes in rice samples

Date: Dec 25, 2017

Author: separationsNOW

Chemists in Iran coated a nanocomposite film onto a stainless steel fiber for solid-phase microextraction of linear aliphatic aldehydes from rice samples, prior to analysis by gas chromatography.

Read More thumbnail image: Journal Highlight A platinized stainless steel fiber with in-situ coated polyanilinepolypyrrolegraphene oxide nanocomposite sorbent for headspace SPME of aliphatic aldehydes in rice samples

Journal Highlight: Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane

Date: Dec 25, 2017

Author: separationsNOW

Biochemists from Finland used gel electrophoresis to investigate the role of thylakoid protein complexes in light harvesting by plant leaves.

Read More thumbnail image: Journal Highlight Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane
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