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Extended beer proteome: Allergenic gluten proteins

Date: Jul 15, 2017

Author: Steve Down

The most extensive proteome of lager beer to date has been been derived by scientists in the Czech Republic who made a special effort to identify gluten proteins which might be allergenic.

Read More thumbnail image: Extended beer proteome Allergenic gluten proteins

Weighing up ways of working out log P

Date: Jul 15, 2017

Author: Ryan De Vooght-Johnson

Lipophilicity is a key parameter affecting the activity of potential drug molecules, but there are many methods to calculate it. Scientists from the University of Belgrade examined various techniques for calculating the lipophilicity of a number of benzothiepino[3,2-c]pyridine derivatives, which were potential antifungal drugs.

Read More thumbnail image: Weighing up ways of working out log P

LC-LTQ-Orbitrap sweetly detects antimicrobial residues in honey

Date: Jul 14, 2017

Author: Ryan De Vooght-Johnson

Various antimicrobial compounds can be used in beekeeping, but careful measurement of any residues of these agents in honey is necessary. Scientists from the Laboratory for the Analysis of Organic Compounds in Beirut and the French Agency for the Safety of Food, Environment and Occupational Health in Fougères (Brittany) described the upgrading of a validated LC-MS/MS method for residue determination to a new LC-Linear Ion Trap/Orbitrap method.

Read More thumbnail image: LC-LTQ-Orbitrap sweetly detects antimicrobial residues in honey

Nerve agents detected when fluoridation selected

Date: Jul 14, 2017

Author: Ryan De Vooght-Johnson

The field identification of chemical warfare agents remains a challenge, particularly for less volatile compounds that are not easily detected by GC-MS systems. Japanese scientists from the National Research Institute of Police Science, INFICON’s Yokohama facility and Teikoku Sen-I Co. in Tokyo have devised a novel fluorinating device that converted VX nerve agent and others to more volatile fluorinated compounds to enable detection by GC-MS.

Read More thumbnail image: Nerve agents detected when fluoridation selected

Journal Highlight: Proteomic profile of Mabuya sp. (Squamata: Scincidae) ovary and placenta during gestation

Date: Jul 10, 2017

Author: separationsNOW

Scientists in Colombia have used two-dimensional gel electrophoresis to identify differentially expressed proteins in the ovary and placenta of long-tailed skinks during early, mid and late gestation.

Read More thumbnail image: Journal Highlight Proteomic profile of Mabuya sp_ Squamata Scincidae ovary and placenta during gestation

Journal Highlight: Determination of chlorophenols in water using dispersive liquid–liquid microextraction coupled with water-in-oil microemulsion electrokinetic chromatography in normal stacking mode

Date: Jul 10, 2017

Author: separationsNOW

Chinese chemists have shown that liquid–liquid microextraction can be combined with water-in-oil microemulsion electrokinetic chromatography for the analysis of chlorophenols in water.

Read More thumbnail image: Journal Highlight Determination of chlorophenols in water using dispersive liquidliquid microextraction coupled with water-in-oil microemulsion electrokinetic chromatography in normal stacking mode

Targeting metabolomics: Conducting simultaneous targeted and non-targeted metabolomics

Date: Jul 10, 2017

Author: Jon Evans

By combining anion-exchange chromatography with high-resolution mass spectrometry, Austrian scientists have been able to conduct targeted and non-targeted metabolomics at the same time.

Read More thumbnail image: Targeting metabolomics Conducting simultaneous targeted and non-targeted metabolomics

Journal Highlight: Synergetic use of principal component analysis applied to normed physicochemical measurements and GC × GC-MS to reveal the stabilization effect of selected essential oils on heated rapeseed oil

Date: Jul 10, 2017

Author: separationsNOW

The potential antioxidant effect of corainder and nutmeg essential oils on the thermal stability of rapeseed oil was determined by GCxGC/MS with PCA.

Read More thumbnail image: Journal Highlight Synergetic use of principal component analysis applied to normed physicochemical measurements and GC  GC-MS to reveal the stabilization effect of selected essential oils on heated rapeseed oil

Journal Highlight: A qualitative and quantitative evaluation of the peptide characteristics of microwave- and ultrasound-assisted digestion in discovery and targeted proteomic analyses

Date: Jul 10, 2017

Author: separationsNOW

Three digestion methods, including overnight digestion, microwave-assisted protein enzymatic digestion and high-intensity focused ultrasonic-assisted enzymatic digestion, in trypsin or in trypsin/Lys-C, were compared for discovery and targeted proteomics analysis using the HeLa cell proteome.

Read More thumbnail image: Journal Highlight A qualitative and quantitative evaluation of the peptide characteristics of microwave- and ultrasound-assisted digestion in discovery and targeted proteomic analyses

Journal Highlight: Compound-specific hydrogen isotope analysis of fluorine-, chlorine-, bromine- and iodine-bearing organics using GC–chromium-based high-temperature conversion isotope ratio MS

Date: Jul 10, 2017

Author: separationsNOW

Scientists from Germany and the US have used chromium-based high-temperature conversion isotope ratio mass spectrometry with gas chromatography to analyse the hydrogen isotopes in halogen-bearing organics.

Read More thumbnail image: Journal Highlight Compound-specific hydrogen isotope analysis of fluorine- chlorine- bromine- and iodine-bearing organics using GCchromium-based high-temperature conversion isotope ratio MS
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