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Journal Highlight: Untargeted metabolomic profiling of seminal plasma in nonobstructive azoospermia men: A noninvasive detection of spermatogenesis

Date: Aug 8, 2017

Author: separationsNOW

Untargeted metabolomic profiling of the seminal plasma in men with nonobstructive azoospermia was studied by GC/MS and advance chemometrics, leading to 36 potential biomarkers of the condition.

Read More thumbnail image: Journal Highlight Untargeted metabolomic profiling of seminal plasma in nonobstructive azoospermia men A noninvasive detection of spermatogenesis

Journal Highlight: The effect of pH and amino acids on the formation of methylglyoxal in a glucose–amino acid model system

Date: Aug 7, 2017

Author: separationsNOW

Chinese researchers have used anion-exchange chromatography to help investigate the effect of amino acids and pH on the formation of methylglyoxal during the Maillard reaction, which is the process that browns food during cooking.

Read More thumbnail image: Journal Highlight The effect of pH and amino acids on the formation of methylglyoxal in a glucoseamino acid model system

Journal Highlight: Separation of enilconazole enantiomers in CE with cyclodextrin-type chiral selectors and investigation of structure of selector-selectand complexes by using NMR spectroscopy

Date: Aug 7, 2017

Author: separationsNOW

An international team of scientists used nuclear magnetic resonance spectroscopy to study the separation of enantiomers of the chiral fungicide enilconazole by capillary electrophoresis in the presence of various cyclodextrins.

Read More thumbnail image: Journal Highlight Separation of enilconazole enantiomers in CE with cyclodextrin-type chiral selectors and investigation of structure of selector-selectand complexes by using NMR spectroscopy

Extended human proteome: Multishot procedure for single cell types

Date: Aug 1, 2017

Author: Steve Down

A novel strategy for deriving comprehensive human proteomes that relies on the offline HPLC separation of multiple injections of protein digests followed by fast scanning tandem mass spectrometry has identified more than 14,000 protein isoforms from a single human cell line.

Read More thumbnail image: Extended human proteome Multishot procedure for single cell types

Orbitrap defeats the athletes who cheat

Date: Aug 1, 2017

Author: Ryan De Vooght-Johnson

Detection of the increasing number of compounds used as doping agents by sport competitors represents a significant challenge. Scientists from the Brazilian Doping Control Laboratory at the Federal University of Rio de Janeiro and Thermo Fisher Scientific (Austin, Texas) have developed a new Orbitrap GC-MS method for detecting a variety of doping compounds.

Read More thumbnail image: Orbitrap defeats the athletes who cheat

Hydrophilic interactions change along with volume fractions

Date: Aug 1, 2017

Author: Ryan De Vooght-Johnson

Hydrophilic interaction liquid chromatography (HILIC) is a relatively new method, so theoretical work on the relationships between its parameters is not as extensive as with older techniques. Scientists from the University of Belgrade looked at the association between peak retention and mobile phase composition for HILIC on the antipsychotic drug olanzapine and its impurities using various theoretical models.

Read More thumbnail image: Hydrophilic interactions change along with volume fractions

Monoclonal measured by means of peptide marker

Date: Aug 1, 2017

Author: Ryan De Vooght-Johnson

Monoclonal antibodies are being increasingly used to treat a wide range of diseases, but quantification of their concentrations in blood remains a challenge. Researchers from Naples have used LC-MS to detect a characteristic peptide fragment from the anticancer monoclonal antibody trastuzumab (Herceptin) in order to determine its serum concentration.

Read More thumbnail image: Monoclonal measured by means of peptide marker

Journal Highlight: Microproteomics with microfluidic-based cell sorting: Application to 1000 and 100 immune cells

Date: Jul 31, 2017

Author: separationsNOW

A microproteomics workflow for limited numbers of immune cells using non-damaging, microfluidic chip-based cell sorting and MS-based proteomics gave reasonable proteome coverage for 100 sorted cells and comprehensive data from 1000 sorted cells.

Read More thumbnail image: Journal Highlight Microproteomics with microfluidic-based cell sorting Application to 1000 and 100 immune cells

Journal Highlight: Determination of selected antibiotics in farmed fish species using LC-MS/MS

Date: Jul 24, 2017

Author: separationsNOW

37 Antibiotics from different classes have been measured in three different farmed fish species by a novel LC-MS/MS method, revealing potential public health concerns.

Read More thumbnail image: Journal Highlight Determination of selected antibiotics in farmed fish species using LC-MSMS

Journal Highlight: Preparation of magnetic microsphere-gold nanoparticle-immobilized enzyme batch reactor and its application to enzyme inhibitor screening in natural extracts by CE

Date: Jul 24, 2017

Author: separationsNOW

By immobilizing enzymes on magnetic microspheres and gold nanoparticles in a reactor, Chinese scientists have developed a novel capillary electrophoresis method for screening the enzyme inhibitors from natural products.

Read More thumbnail image: Journal Highlight Preparation of magnetic microsphere-gold nanoparticle-immobilized enzyme batch reactor and its application to enzyme inhibitor screening in natural extracts by CE
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