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Caught in the moment: Investigating molecular interactions with affinity capillary electrophoresis

Date: Sep 17, 2018

Author: Jon Evans

Japanese scientists have shown that affinity capillary electrophoresis can reveal even more information about the interactions between molecules than originally supposed.

Read More thumbnail image: Caught in the moment Investigating molecular interactions with affinity capillary electrophoresis

Journal Highlight: Enantiomeric identification of pregabalin by GC‐MS via methylation and S‐TPC chiral derivatization

Date: Sep 17, 2018

Author: separationsNOW

Problems associated with the traditional GC‐MS analysis of pregabalin have been overcome by methylating the carboxylic acid group followed by chiral derivatization at the amine group to identify the (S) enantiomer which is a controlled substance.

Read More thumbnail image: Journal Highlight Enantiomeric identification of pregabalin by GCMS via methylation and STPC chiral derivatization

Mutated genes are plain to see using dHPLC

Date: Sep 12, 2018

Author: Ryan De Vooght-Johnson

Denaturing HPLC (dHPLC) uses ion-pair reversed-phase HPLC to separate homoduplex and heteroduplex DNA molecules, but typically produces large amounts of data. Scientists from the Institute of Technology and Higher Education (Monterrey, Mexico) and the ‘ISSSTESON’ institute (Hermosillo, Mexico) used dHPLC in combination with principal component analysis (PCA) and box plots to detect DNA point mutations associated with cancer.

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Fishing for allergens: With ion-exchange chromatography and capillary electrophoresis

Date: Sep 10, 2018

Author: Jon Evans

Chinese scientists have combined ion-exchange chromatography with capillary electrophoresis to produce a quick and simple method for detecting the main allergen in fish.

Read More thumbnail image: Fishing for allergens With ion-exchange chromatography and capillary electrophoresis

Journal Highlight: Biomarkers of individual foods, and separation of diets using untargeted LC–MS‐based plasma metabolomics in a randomized controlled trial

Date: Sep 10, 2018

Author: separationsNOW

The effect of the new Nordic diet versus average Danish diet on plasma metabolic profiles has been investigated by LC/MS to identify biomarkers of diet compliance and metabolic effects, using supervised machine learning with feature selection.

Read More thumbnail image: Journal Highlight Biomarkers of individual foods and separation of diets using untargeted LCMSbased plasma metabolomics in a randomized controlled trial

Journal Highlight: Salt‐stress induced proteomic changes of two contrasting alfalfa cultivars during germination stage

Date: Sep 10, 2018

Author: separationsNOW

Chinese scientists used two-dimensional gel electrophoresis to compare the proteomes of alfalfa cultivars that differ in their tolerance to salt stress.

Read More thumbnail image: Journal Highlight Saltstress induced proteomic changes of two contrasting alfalfa cultivars during germination stage

Journal Highlight: Optimized extraction of secoiridoid glycosides from Gentianae radix by pressurized liquid extraction using a Box–Behnken Design

Date: Sep 10, 2018

Author: separationsNOW

Chinese scientists used a Box–Behnken design to optimize experimental conditions for the pressurized liquid extraction of three secoiridoid glycosides from the flowing plant Gentianae radix, prior to analysis by high-performance liquid chromatography.

Read More thumbnail image: Journal Highlight Optimized extraction of secoiridoid glycosides from Gentianae radix by pressurized liquid extraction using a BoxBehnken Design

Journal Highlight: Determination of glucosamine and monitoring of its mutarotation by HILIC with ELSD

Date: Sep 10, 2018

Author: separationsNOW

A chemist from the Czech Republic has developed a novel procedure for detecting saccharides and their derivates with an evaporative light scattering detector after separation by high-performance liquid chromatography.

Read More thumbnail image: Journal Highlight Determination of glucosamine and monitoring of its mutarotation by HILIC with ELSD

Journal Highlight: Monolithic column functionalized with quinine derivative for anion‐exchange capillary electrochromatography

Date: Sep 5, 2018

Author: separationsNOW

Chinese scientists have developed a novel anion‐exchange organic polymer monolithic column based on the monomers N‐benzylquininium chloride and acrylamide for performing capillary electrochromatography.

Read More thumbnail image: Journal Highlight Monolithic column functionalized with quinine derivative for anionexchange capillary electrochromatography

Journal Highlight: HPLC associated with resonance Rayleigh scattering for synchronous determination of three antihistamines and mechanism study

Date: Sep 5, 2018

Author: separationsNOW

Chinese scientists have combined resonance Rayleigh scattering with high performance liquid chromatography to produce a novel method for detecting antihistamine drugs in water samples.

Read More thumbnail image: Journal Highlight HPLC associated with resonance Rayleigh scattering for synchronous determination of three antihistamines and mechanism study
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