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Journal Highlight: Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane

Date: Dec 25, 2017

Author: separationsNOW

Biochemists from Finland used gel electrophoresis to investigate the role of thylakoid protein complexes in light harvesting by plant leaves.

Read More thumbnail image: Journal Highlight Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane

Colorful detection: Coupling colorimetry with capillary electrophoresis

Date: Dec 18, 2017

Author: Jon Evans

By slotting three capillaries inside each other, Chinese scientists have found a way to combine capillary electrophoresis with gold nanoparticle-based colorimetry.

Read More thumbnail image: Colorful detection Coupling colorimetry with capillary electrophoresis

Crossing the streams: Switching solvents with free-flow electrophoresis

Date: Dec 18, 2017

Author: Jon Evans

German scientists have shown how free-flow electrophoresis can be used to move analytes from one solvent to another in lab-on-a-chip devices.

Read More thumbnail image: Crossing the streams Switching solvents with free-flow electrophoresis

Taking the plume plunge: Analyzing amino acids in a jet of alien water

Date: Dec 18, 2017

Author: Jon Evans

Capturing amino acids from plumes of water escaping from an icy moon of Saturn may be quite a challenge, but extracting the amino acids for analysis by microchip electrophoresis turns out to be surprisingly easy.

Read More thumbnail image: Taking the plume plunge Analyzing amino acids in a jet of alien water

Journal Highlight: Diagnostic accuracy of increased urinary cortisol/cortisone ratio to differentiate ACTH-dependent Cushing's syndrome

Date: Dec 18, 2017

Author: separationsNOW

HPLC-UV and LC-MS/MS have been used to measure urinary free cortisol and cortisone and the cortisol to cortisone ratio in patients with Cushing's disease and ectopic ACTH syndrome to improve diagnostic accuracy.

Read More thumbnail image: Journal Highlight Diagnostic accuracy of increased urinary cortisolcortisone ratio to differentiate ACTH-dependent Cushings syndrome

Amyloidosis classification: MALDI mass spectrometric imaging

Date: Dec 15, 2017

Author: Steve Down

Scientists in Germany have used mass spectrometry imaging coupled with ion mobility separation to identify amyloid peptides in a proof-of-concept study that will allow different types of amyloidosis to be classified.

Read More thumbnail image: Amyloidosis classification MALDI mass spectrometric imaging

GC defeats AICAR sports cheats

Date: Dec 14, 2017

Author: Ryan De Vooght-Johnson

AICAR is a substance that occurs naturally in the body, but can also be used as a performance-enhancing drug in sports. Scientists from the French Anti-Doping Agency in Paris have developed an improved method for distinguishing natural AICAR from that derived from doping using GC-C-IRMS (gas chromatography, combustion, isotope ratio mass spectrometry).

Read More thumbnail image: GC defeats AICAR sports cheats

Chemometrics shown to fix complexity of herbal mix

Date: Dec 14, 2017

Author: Ryan De Vooght-Johnson

Lianqiao Baidu pills are a popular traditional Chinese medicine made from a mixture of herbal ingredients, but up to now there has been no accurate way to analyse their quality. Researchers from Shenyang Pharmaceutical University in China categorised the quality of this material using extraction, HPLC, measurements of its antioxidant power and chemometric methods.

Read More thumbnail image: Chemometrics shown to fix complexity of herbal mix

Acetic acid additive best for negative ESI

Date: Dec 13, 2017

Author: Ryan De Vooght-Johnson

There is little consensus about the best additives to use for LC-MS negative electrospray ionisation (negative ion ESI). Scientists from Concordia University, Montreal, compared the effects of acetic acid, ammonium hydroxide and ammonium acetate on LC-MS negative ion ESI using mixtures of lipid standards and also extracted human lipids.

Read More thumbnail image: Acetic acid additive best for negative ESI

Journal Highlight: Determination of cyclic volatile methylsiloxanes in personal care products by gas chromatography

Date: Dec 11, 2017

Author: separationsNOW

A simple GC-FID method has been developed to enable accurate measurement of low levels of octamethylcyclotetrasiloxane (D4) and decamethylcyclotetrasiloxane (D5) in personal care products.

Read More thumbnail image: Journal Highlight Determination of cyclic volatile methylsiloxanes in personal care products by gas chromatography
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