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Journal Highlight: Simultaneous determination of glycol ethers and their acetates in cosmetics by GC–MS

Date: Apr 16, 2018

Author: separationsNOW

A GC/MS method has been developed for the simultaneous determination of ten kinds of toxic glycol ethers and their acetates in cosmetics, with method validation from different laboratories.

Read More thumbnail image: Journal Highlight Simultaneous determination of glycol ethers and their acetates in cosmetics by GCMS

Anton Checkov: Cause of death questioned

Date: Apr 15, 2018

Author: Steve Down

Proteome analysis of brown stains on archived personal items of Anton Checkov has yielded proteins related to Mycobacterium tuberculosis, confirming that he had tuberculosis at time of death, but another protein suggests that there may have been a different cause of death.

Read More thumbnail image: Anton Checkov Cause of death questioned

Thinking inside the box: A novel alternative to preparative chromatography

Date: Apr 9, 2018

Author: Jon Evans

Canadian researchers show that a box filled with ion-exchange particles offers a more efficient way to purify protein-based biopharmaceuticals than conventional preparative chromatography.

Read More thumbnail image: Thinking inside the box A novel alternative to preparative chromatography

Liquorice lessens alarming alkaloid levels

Date: Apr 9, 2018

Author: Ryan De Vooght-Johnson

The seeds of the tree Strychnos nux-vomica (‘Semen Strychni’), are used in traditional Chinese medicine, despite containing the poisonous alkaloids strychnine and brucine. Scientists from the Central South University (Changsha, China) used LC-MS to examine alkaloids from ‘Semen Strychni’ and compounds from liquorice, since it has been claimed that liquorice can reduce the toxicity of these seeds.

Read More thumbnail image: Liquorice lessens alarming alkaloid levels

Fungicide assay by GC gives good results with ECD

Date: Apr 9, 2018

Author: Ryan De Vooght-Johnson

The fungicide dimethachlon is widely used on rice plants in China, despite its relatively high level of kidney toxicity. Scientists from the Guizhou University (Guiyang, China) developed a new GC method using electron capture detection (ECD) to detect dimethachlon residues in paddy soil, rice straw, rice husks and rice grains.

Read More thumbnail image: Fungicide assay by GC gives good results with ECD

Journal Highlight: Metabolite identification of the antimalarial naphthoquine using liquid chromatography–tandem high‐resolution mass spectrometry in combination with multiple data‐mining tools

Date: Apr 9, 2018

Author: separationsNOW

Metabolite profiling of naphthoquine has been studied in six liver microsomal incubates, seven recombinant CYP enzymes and the rat model by LC-HRMSn in conjunction with online H/D exchange and multiple data‐mining tools applied in tandem.

Read More thumbnail image: Journal Highlight Metabolite identification of the antimalarial naphthoquine using liquid chromatographytandem highresolution mass spectrometry in combination with multiple datamining tools

2D GC defeats 1D on compounds from the water flea

Date: Apr 6, 2018

Author: Ryan De Vooght-Johnson

Two-dimensional GC, where the output of one column is put through another, is a powerful technique that is being increasingly applied to complex mixtures of metabolites. Scientists from the Sharif University of Technology (Tehran) and IDAEA-CSIC (a government institute in Barcelona) compared the effectiveness of ordinary GC-MS with that of two dimensional GC plus time-of-flight MS for metabolite detection in Daphnia (water fleas), employing chemometric techniques to manipulate the data.

Read More thumbnail image: 2D GC defeats 1D on compounds from the water flea

Journal Highlight: 'One‐pot' sample processing method for proteome‐wide analysis of microbial cells and spores

Date: Apr 3, 2018

Author: separationsNOW

A simple 'one‐pot' method for the proteome analysis of bacterial spores has been demonstrated for Bacillus subtilis, Bacillus cereus and Peptoclostridium difficile, yielding to the identification of >1000 across all spore layers of the three organisms.

Read More thumbnail image: Journal Highlight Onepot sample processing method for proteomewide analysis of microbial cells and spores

Journal Highlight: Plasma metabolome analysis of patients with major depressive disorder

Date: Apr 2, 2018

Author: separationsNOW

Japanese researchers used ion chromatography to measure levels of the metabolite phosphoethanolamine in blood plasma, as a candidate biomarker of major depressive disorder.

Read More thumbnail image: Journal Highlight Plasma metabolome analysis of patients with major depressive disorder

Asthma at work: Proteomics search for biomarkers

Date: Apr 1, 2018

Author: Steve Down

Nasal protein profiling has been used to measure the responses of people exposed to airborne allergens like isocyanates and welding fumes, leading to several candidate biomarkers for work-related asthma.

Read More thumbnail image: Asthma at work Proteomics search for biomarkers
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