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Journal Highlight: A robust protocol for the isolation of cellular proteins from Xanthomonas campestris to analyze the methionine effect in 2D-gel experiments

Date: Oct 30, 2017

Author: separationsNOW

A phenol extraction protocol developed for the purification of bacterial proteins that provides excellent 2D gel separation has been illustrated by examining the effects of methionine on the cellular proteome of Xanthomonas campestris.

Read More thumbnail image: Journal Highlight A robust protocol for the isolation of cellular proteins from Xanthomonas campestris to analyze the methionine effect in 2D-gel experiments

Journal Highlight: Tryptophan and cysteine oxidation products dominate in α-lactalbumin-derived peptides analyzed with LC–MSn

Date: Oct 23, 2017

Author: separationsNOW

The oxidation products of tryptophan and cysteine residues in α-lactalbumin, a major milk whey protein, have been identified, which will help to monitor the safety of protein-containing foods, including food supplements for sports nutrition.

Read More thumbnail image: Journal Highlight Tryptophan and cysteine oxidation products dominate in -lactalbumin-derived peptides analyzed with LCMSn

Journal Highlight: Probing skin physiology through the volatile footprint: Discriminating volatile emissions before and after acute barrier disruption

Date: Oct 23, 2017

Author: separationsNOW

Researchers in Ireland have used a small wearable housing integrating a solid-phase micro-extraction fiber, together with gas chromatography-mass spectrometry, to analyze the volatile organic compounds emitted by human skin.

Read More thumbnail image: Journal Highlight Probing skin physiology through the volatile footprint Discriminating volatile emissions before and after acute barrier disruption

Journal Highlight: Lipid profiling of parkin-mutant human skin fibroblasts

Date: Oct 23, 2017

Author: separationsNOW

Scientists in Italy used thin-layer chromatography to investigate possible lipid alterations associated with the lack of a protein called parkin, mutations of which are a major cause of early-onset Parkinson's disease, in skin primary fibroblasts.

Read More thumbnail image: Journal Highlight Lipid profiling of parkin-mutant human skin fibroblasts

Journal Highlight: A comparative proteomic analysis of blood serum for developmental stages in pigs

Date: Oct 23, 2017

Author: separationsNOW

Korean scientists have used two-dimensional gel electrophoresis to study how protein expression changes as pigs grow and develop over the first 150 days from birth.

Read More thumbnail image: Journal Highlight A comparative proteomic analysis of blood serum for developmental stages in pigs

More than the sum of its parts: Detecting endotoxins with SPE, CE and LIF

Date: Oct 16, 2017

Author: Jon Evans

A combination of solid-phase extraction, capillary electrophoresis and laser-induced fluorescence detection produces a sensitive and highly selective method for detecting bacterial endotoxins.

Read More thumbnail image: More than the sum of its parts Detecting endotoxins with SPE CE and LIF

A gap opens up: Analyzing protein binding with CE and surface plasmon resonance

Date: Oct 16, 2017

Author: Jon Evans

To analyze protein binding with capillary electrophoresis and surface plasmon resonance, Dutch scientists found they needed to scratch a gap into a gold sensor.

Read More thumbnail image: A gap opens up Analyzing protein binding with CE and surface plasmon resonance

ELISA meet NLISA: A novel immunosorbent assay with gel electrophoresis

Date: Oct 16, 2017

Author: Jon Evans

A novel form of immunosorbent assay utilizing gel electrophoresis is faster, more sensitive and less prone to false detections than existing versions, and could form the basis for quick and cheap medical diagnosis devices.

Read More thumbnail image: ELISA meet NLISA A novel immunosorbent assay with gel electrophoresis

Journal Highlight: GC-MS metabolomics-based approach for the identification of a potential VOC-biomarker panel in the urine of renal cell carcinoma patients

Date: Oct 16, 2017

Author: separationsNOW

The urinary volatile metabolic profile of patients with renal cell carcinoma was studied by GC/MS to identify a potential specific volatile pattern as a non-invasive strategy to detect the disease.

Read More thumbnail image: Journal Highlight GC-MS metabolomics-based approach for the identification of a potential VOC-biomarker panel in the urine of renal cell carcinoma patients

Influenza A proteins: Individual proteome variations

Date: Oct 15, 2017

Author: Steve Down

Scientists in the US have shown that the proteomes of normal human bronchial epithelial cells are unique for individual people and also respond to infection by the influenza A virus in different ways, findings which could be used to identify people more susceptible to the disease.

Read More thumbnail image: Influenza A proteins Individual proteome variations
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