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Journal Highlight: Extraction, isolation, and concentration of calprotectin antigen from granulocytes

Date: May 7, 2018

Author: separationsNOW

Scientists from Sweden and Norway used ion-exchange chromatography to purify calprotectin, a promising biomarker for inflammatory bowel disease, from granulocyte extracts.

Read More thumbnail image: Journal Highlight Extraction isolation and concentration of calprotectin antigen from granulocytes

GC on waste streams gives ‘speed’ reaction schemes

Date: May 7, 2018

Author: Ryan De Vooght-Johnson

Waste solutions from illicit amphetamine production need to be analysed, both in order to provide useful intelligence for enforcement agencies, and also because they are a potential environmental hazard. A team of scientists from Germany (University of Rostock and the Federal Criminal Police Office, Wiesbaden) and the Netherlands (Netherlands Forensic Institute, The Hague) used solid-phase extraction and GC to examine the compounds in waste solutions produced by different variations of the Leuckart route...

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Fatty acids from foamy films found by TOF and TWIMS

Date: May 4, 2018

Author: Ryan De Vooght-Johnson

The sea surface microlayer (SML), roughly the top millimetre of the ocean, contains relatively high concentrations of organic compounds, including fatty acids. Scientists from the University of Stockholm used a combination of UHPLC, time-of-flight mass spectrometry (TOF-MS) and travelling-wave ion mobility spectrometry (TWIMS) to separate and identify a complex mixture of fatty acids from the Arctic Ocean SML.

Read More thumbnail image: Fatty acids from foamy films found by TOF and TWIMS

NIST spectral library matching was poor, puzzling Livermore

Date: May 3, 2018

Author: Ryan De Vooght-Johnson

The NIST library is routinely used for identifying compounds from their mass spectra, but occasionally the matches between experimental and library data are poor. Researchers from the Lawrence Livermore National Laboratory (Livermore, California) found that an impurity in a sample of a chemical incapacitating agent gave a mass spectrum that differed significantly from that in the NIST library.

Read More thumbnail image: NIST spectral library matching was poor puzzling Livermore

The force of Orbitrap can thwart doping from the dark side of sport

Date: May 2, 2018

Author: Ryan De Vooght-Johnson

The use of anabolic steroids by athletes for doping purposes remains a serious problem, but existing analytical methods tend to give poor detection of some steroids, particularly novel ‘designer steroids’, which are often not found in databases. Researchers from the Federal University of Rio de Janeiro and Thermo Fisher (Austin, Texas) devised a new method using GC and an Orbitrap mass spectrometer to detect both known and novel anabolic steroids, along with other doping compounds.

Read More thumbnail image: The force of Orbitrap can thwart doping from the dark side of sport

Postmortem interval: Forensic proteomic pointers

Date: May 1, 2018

Author: Steve Down

A proteomics study of decaying bones has thrown up a number of proteins that might prove to be valuable markers of the skeletal postmortem interval as an aid to forensic scientists.

Read More thumbnail image: Postmortem interval Forensic proteomic pointers

Journal Highlight: Cell‐free DNA blood collection tubes are appropriate for clinical proteomics: A demonstration in colorectal cancer

Date: Apr 30, 2018

Author: separationsNOW

A proteomics study of colorectal cancer patients has shown that optimized blood collection tubes are appropriate for low‐abundant plasma protein analysis and can be used with confidence for clinical proteomics.

Read More thumbnail image: Journal Highlight Cellfree DNA blood collection tubes are appropriate for clinical proteomics A demonstration in colorectal cancer

Journal Highlight: Detection of trace amounts of citrinin in dried orange peel by using an optimized extraction method coupled with ultra‐performance liquid chromatography–tandem mass spectrometry

Date: Apr 23, 2018

Author: separationsNOW

A fast and sensitive method involving ultra‐performance liquid chromatography–tandem mass spectrometry has been introduced to detect the mycotoxin citrinin in dried orange peel.

Read More thumbnail image: Journal Highlight Detection of trace amounts of citrinin in dried orange peel by using an optimized extraction method coupled with ultraperformance liquid chromatographytandem mass spectrometry

Viral VOCs: Optimizing the extraction of VOCs from infected cells

Date: Apr 16, 2018

Author: Jon Evans

Researchers in the US have developed an optimized solid-phase microextraction method for extracting volatile organic compounds from cells infected with viruses, as a way of diagnosing viral diseases.

Read More thumbnail image: Viral VOCs Optimizing the extraction of VOCs from infected cells

Illuminating fingerprints: A novel fluorescence-based system for detecting proteins

Date: Apr 16, 2018

Author: Jon Evans

Indian scientists have developed a fluorescence-based system that can reveal a whole load of information about proteins separated by gel electrophoresis while they’re still embedded in the gel.

Read More thumbnail image: Illuminating fingerprints A novel fluorescence-based system for detecting proteins
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