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Journal Highlight: Flow cytometry and CE analyses in ethanol-stressed Oenococcus oeni strains and changes assessment of membrane fatty acid composition

Date: Jun 12, 2017

Author: separationsNOW

Italian scientists have used capillary electrophoresis and flow cytometry to investigate the dynamics and physiological heterogeneity of the bacterium Oenococcus oeni when exposed to ethanol.

Read More thumbnail image: Journal Highlight Flow cytometry and CE analyses in ethanol-stressed Oenococcus oeni strains and changes assessment of membrane fatty acid composition

Journal Highlight: Combination of SDS-PAGE and intact mass analysis for rapid determination of heterogeneities in monoclonal antibody therapeutics

Date: Jun 12, 2017

Author: separationsNOW

Pharmaceutical scientists in Japan have shown that a combination of gel electrophoresis and intact mass analysis can play a pivotal role in the rapid and precise characterization of monoclonal antibodies.

Read More thumbnail image: Journal Highlight Combination of SDS-PAGE and intact mass analysis for rapid determination of heterogeneities in monoclonal antibody therapeutics

Journal Highlight: Validation of analytical method for α-dicarbonyl compounds using gas chromatography–nitrogen phosphorous detector and their levels in alcoholic beverages

Date: Jun 12, 2017

Author: separationsNOW

A GC-NPD method has been developed to measure α-dicarbonyl compounds including glyoxal, methylglyoxal and diacetyl in 101 alcoholic beverages then perform exposure assessments.

Read More thumbnail image: Journal Highlight Validation of analytical method for -dicarbonyl compounds using gas chromatographynitrogen phosphorous detector and their levels in alcoholic beverages

Journal Highlight: Proteomics applications in dental derived stem cells

Date: Jun 10, 2017

Author: separationsNOW

An overview has been presented of proteomics studies in the context of stem cell research, focusing on dental-derived stem cell types as well as current challenges and opportunities.

Read More thumbnail image: Journal Highlight Proteomics applications in dental derived stem cells

Journal Highlight: QSAR models for prediction of chromatographic behavior of homologous Fab variants

Date: Jun 7, 2017

Author: separationsNOW

Scientists from the US and Denmark have developed quantitative structure activity relationship models for protein separation in six chromatographic systems, including multimodal chromatography and cation-exchange chromatography.

Read More thumbnail image: Journal Highlight QSAR models for prediction of chromatographic behavior of homologous Fab variants

Journal Highlight: Determination of chlorate, perchlorate and bromate anions in water samples by microbore reversed-phase LC coupled to sonic-spray ionization MS

Date: Jun 7, 2017

Author: separationsNOW

Greek chemists have combined liquid chromatography with sonic-spray ionization mass spectrometry to analyze inorganic anions in water samples.

Read More thumbnail image: Journal Highlight Determination of chlorate perchlorate and bromate anions in water samples by microbore reversed-phase LC coupled to sonic-spray ionization MS

Journal Highlight: Essential-oil inspired synthetic libraries: synthesis, spectral and gas-chromatographic properties of new fenchyl esters

Date: Jun 5, 2017

Author: separationsNOW

The design, synthesis, and GC and NMR spectral characterization of a library of 32 endo- and exo-fenchyl esters have been described in work which will facilitate future essential oil analyses, especially those involving minor constituents.

Read More thumbnail image: Journal Highlight Essential-oil inspired synthetic libraries synthesis spectral and gas-chromatographic properties of new fenchyl esters

Gulf War Illness: Proteomics clues to affected biological pathways

Date: Jun 1, 2017

Author: Steve Down

Two complementary proteomics experiments have been carried out on the mouse model of Gulf War Illness to identify modified biological pathways in the central nervous system that might lead to targets for novel therapeutic agents.

Read More thumbnail image: Gulf War Illness Proteomics clues to affected biological pathways

Sensitising compounds caught by chemiluminescence chromatography

Date: Jun 1, 2017

Author: Ryan De Vooght-Johnson

Linalyl acetate is found in a number of essential oils, such as those of lavender and bergamot, and in many perfumery products. However, its hydroperoxides have been implicated as sensitising agents. Scientists from the fragrance company Firmenich, based at their Plainsboro New Jersey site, used HPLC combined with chemiluminescence to detect these hydroperoxides.

Read More thumbnail image: Sensitising compounds caught by chemiluminescence chromatography

Heart-cutting GC stops citrus fakery

Date: Jun 1, 2017

Author: Ryan De Vooght-Johnson

Citrus fruits and their products, such as essential oils, are found in a wide range of foods and perfumery formulations, but can be adulterated with synthetic compounds or cheaper natural extracts. Scientists from South Korea and Pakistan used heart-cutting multi-dimensional gas chromatography and mass spectrometry to determine the enantiomeric excess of various citrus compounds, measurements of which should detect adulteration.

Read More thumbnail image: Heart-cutting GC stops citrus fakery
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