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Oral squamous cell carcinoma: Dual protein biomarkers

Date: Jan 15, 2018

Author: Steve Down

A combination of two proteins found in saliva can predict oral squamous cell carcinoma and are unaffected by the risk factors human papillomavirus and Epstein-Barr virus, leading to the possibility of a clinical test for the cancer.

Read More thumbnail image: Oral squamous cell carcinoma Dual protein biomarkers

Beneficial bream bacteria biosurfactants: Analyzing bioactive compounds in bream guts

Date: Jan 8, 2018

Author: Jon Evans

Using various analytical techniques, including thin-layer chromatography, Italian scientists have discovered that certain bacteria in the guts of sea bream can produce biosurfactants with some highly useful properties.

Read More thumbnail image: Beneficial bream bacteria biosurfactants Analyzing bioactive compounds in bream guts

Journal Highlight: Sample preparation for large-scale bioanalytical studies based on liquid chromatographic techniques

Date: Jan 8, 2018

Author: separationsNOW

In this literature review, chemists from Romania detail the main sample preparation techniques used for large-scale bioanalytical studies with liquid chromatography, including protein precipitation, liquid–liquid extraction, solid-phase extraction and derivatization.

Read More thumbnail image: Journal Highlight Sample preparation for large-scale bioanalytical studies based on liquid chromatographic techniques

Journal Highlight: Mass spectrometry in untargeted LC/MS metabolomics: Electrospray ionisation parameters and global coverage of the metabolome

Date: Jan 8, 2018

Author: separationsNOW

Biochemists from South Africa have investigated the electronic factors, including capillary and sample cone voltages, relating to the functioning of the electrospray ionization source in metabolomics studies with liquid chromatography/mass spectrometry.

Read More thumbnail image: Journal Highlight Mass spectrometry in untargeted LCMS metabolomics Electrospray ionisation parameters and global coverage of the metabolome

Journal Highlight: Comparative proteomic analysis of Xanthomonas citri ssp. citri periplasmic proteins reveals changes in cellular envelope metabolism during in vitro pathogenicity induction

Date: Jan 8, 2018

Author: separationsNOW

Brazilian scientists have used two-dimensional gel electrophoresis to identify proteins associated with the virulence of the bacterium responsible for citrus canker, a disease of citrus plants such as lime.

Read More thumbnail image: Journal Highlight Comparative proteomic analysis of Xanthomonas citri ssp_ citri periplasmic proteins reveals changes in cellular envelope metabolism during in vitro pathogenicity induction

Journal Highlight: Metabolite profiling of peppers of various colors reveals relationships between tocopherol, carotenoid, and phytosterol content

Date: Jan 8, 2018

Author: separationsNOW

The contents of various metabolites in peppers of various colors (green, red, pale green, and violet peppers) and in a high-pungency (green) pepper have been measured and correlated using PCA, Pearson's correlation analysis and hierarchical clustering analysis.

Read More thumbnail image: Journal Highlight Metabolite profiling of peppers of various colors reveals relationships between tocopherol carotenoid and phytosterol content

Journal Highlight: Routine quantification of 54 allergens in fragrances using comprehensive GCxGC-QMS with dual parallel secondary columns

Date: Jan 5, 2018

Author: separationsNOW

Scientists in Switzerland and Italy have combined two-dimensional gas chromatography with both a quadrupole mass spectrometer and a flame ionization detector to produce a novel method for analyzing allergens in fragrances.

Read More thumbnail image: Journal Highlight Routine quantification of 54 allergens in fragrances using comprehensive GCxGC-QMS with dual parallel secondary columns

Journal Highlight: Characterization of a robust serine protease from Bacillus subtilis K-1

Date: Jan 5, 2018

Author: separationsNOW

Researchers in India used ion-exchange chromatography and size-exclusion chromatography to extract and purify a robust protease from the bacterium Bacillus subtilis.

Read More thumbnail image: Journal Highlight Characterization of a robust serine protease from Bacillus subtilis K-1

Heart failure: HDL protein signals

Date: Jan 3, 2018

Author: Steve Down

The proteome of high-density lipoprotein is different for survivors of heart failure and those who died, say an international team of researchers who found that a group of 12 proteins gave strong predictive capabilities.

Read More thumbnail image: Heart failure HDL protein signals

Journal Highlight: How many proteins can be identified in a 2-DE gel spot within an analysis of a complex human cancer tissue proteome?

Date: Jan 1, 2018

Author: separationsNOW

Three different mass spectrometric techniques have been compared for the identification of proteins within a single 2-DE gel spot, confirming the necessity of isotopic labeling in large-scale quantification of different protein species in a proteome.

Read More thumbnail image: Journal Highlight How many proteins can be identified in a 2-DE gel spot within an analysis of a complex human cancer tissue proteome
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