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Sample preparation in proteomics: Method bias

Date: May 15, 2018

Author: Steve Down

A comparison of four commonly used proteomics sample preparation methods for head and neck tissues has revealed that there is no universal method, each having their own deficiencies and advantages.

Read More thumbnail image: Sample preparation in proteomics Method bias

Extracellular activities: Isolating extracellular vesicles with anion-exchange chromatography

Date: May 14, 2018

Author: Jon Evans

Researchers from the pharmaceutical giant AstraZeneca have shown that anion-exchange chromatography offers a quick and effective way of isolating extracellular vesicles, which show promise for drug delivery, from cell cultures.

Read More thumbnail image: Extracellular activities Isolating extracellular vesicles with anion-exchange chromatography

Journal Highlight: Prediction of designer drugs: Synthesis and spectroscopic analysis of synthetic cathinone analogs that may appear on the Swedish drug market

Date: May 14, 2018

Author: separationsNOW

Six new synthetic cathinones that have not yet appeared in any Swedish drug seizures have been synthesized and used with 42 known cathinones to show that a combination of GC/MS and GC-FTIR is required for successful differentiation.

Read More thumbnail image: Journal Highlight Prediction of designer drugs Synthesis and spectroscopic analysis of synthetic cathinone analogs that may appear on the Swedish drug market

Journal Highlight: A simple, fast, and accurate thermodynamic‐based approach for transfer and prediction of gas chromatography retention times between columns and instruments Part III: Retention time prediction on target column

Date: May 7, 2018

Author: separationsNOW

Models have been derived for the transfer and prediction of retention time from a reference GC column to the target column using experimental data for a separate mixture of compounds, based on the thermodynamic‐based parameters of the target compound on the reference column.

Read More thumbnail image: Journal Highlight A simple fast and accurate thermodynamicbased approach for transfer and prediction of gas chromatography retention times between columns and instruments Part III Retention time prediction on target column

Journal Highlight: Extraction, isolation, and concentration of calprotectin antigen from granulocytes

Date: May 7, 2018

Author: separationsNOW

Scientists from Sweden and Norway used ion-exchange chromatography to purify calprotectin, a promising biomarker for inflammatory bowel disease, from granulocyte extracts.

Read More thumbnail image: Journal Highlight Extraction isolation and concentration of calprotectin antigen from granulocytes

GC on waste streams gives ‘speed’ reaction schemes

Date: May 7, 2018

Author: Ryan De Vooght-Johnson

Waste solutions from illicit amphetamine production need to be analysed, both in order to provide useful intelligence for enforcement agencies, and also because they are a potential environmental hazard. A team of scientists from Germany (University of Rostock and the Federal Criminal Police Office, Wiesbaden) and the Netherlands (Netherlands Forensic Institute, The Hague) used solid-phase extraction and GC to examine the compounds in waste solutions produced by different variations of the Leuckart route...

Read More thumbnail image: GC on waste streams gives speed reaction schemes

Fatty acids from foamy films found by TOF and TWIMS

Date: May 4, 2018

Author: Ryan De Vooght-Johnson

The sea surface microlayer (SML), roughly the top millimetre of the ocean, contains relatively high concentrations of organic compounds, including fatty acids. Scientists from the University of Stockholm used a combination of UHPLC, time-of-flight mass spectrometry (TOF-MS) and travelling-wave ion mobility spectrometry (TWIMS) to separate and identify a complex mixture of fatty acids from the Arctic Ocean SML.

Read More thumbnail image: Fatty acids from foamy films found by TOF and TWIMS

NIST spectral library matching was poor, puzzling Livermore

Date: May 3, 2018

Author: Ryan De Vooght-Johnson

The NIST library is routinely used for identifying compounds from their mass spectra, but occasionally the matches between experimental and library data are poor. Researchers from the Lawrence Livermore National Laboratory (Livermore, California) found that an impurity in a sample of a chemical incapacitating agent gave a mass spectrum that differed significantly from that in the NIST library.

Read More thumbnail image: NIST spectral library matching was poor puzzling Livermore

The force of Orbitrap can thwart doping from the dark side of sport

Date: May 2, 2018

Author: Ryan De Vooght-Johnson

The use of anabolic steroids by athletes for doping purposes remains a serious problem, but existing analytical methods tend to give poor detection of some steroids, particularly novel ‘designer steroids’, which are often not found in databases. Researchers from the Federal University of Rio de Janeiro and Thermo Fisher (Austin, Texas) devised a new method using GC and an Orbitrap mass spectrometer to detect both known and novel anabolic steroids, along with other doping compounds.

Read More thumbnail image: The force of Orbitrap can thwart doping from the dark side of sport

Postmortem interval: Forensic proteomic pointers

Date: May 1, 2018

Author: Steve Down

A proteomics study of decaying bones has thrown up a number of proteins that might prove to be valuable markers of the skeletal postmortem interval as an aid to forensic scientists.

Read More thumbnail image: Postmortem interval Forensic proteomic pointers
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