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More than the sum of its parts: Detecting endotoxins with SPE, CE and LIF

Date: Oct 16, 2017

Author: Jon Evans

A combination of solid-phase extraction, capillary electrophoresis and laser-induced fluorescence detection produces a sensitive and highly selective method for detecting bacterial endotoxins.

Read More thumbnail image: More than the sum of its parts Detecting endotoxins with SPE CE and LIF

A gap opens up: Analyzing protein binding with CE and surface plasmon resonance

Date: Oct 16, 2017

Author: Jon Evans

To analyze protein binding with capillary electrophoresis and surface plasmon resonance, Dutch scientists found they needed to scratch a gap into a gold sensor.

Read More thumbnail image: A gap opens up Analyzing protein binding with CE and surface plasmon resonance

ELISA meet NLISA: A novel immunosorbent assay with gel electrophoresis

Date: Oct 16, 2017

Author: Jon Evans

A novel form of immunosorbent assay utilizing gel electrophoresis is faster, more sensitive and less prone to false detections than existing versions, and could form the basis for quick and cheap medical diagnosis devices.

Read More thumbnail image: ELISA meet NLISA A novel immunosorbent assay with gel electrophoresis

Journal Highlight: GC-MS metabolomics-based approach for the identification of a potential VOC-biomarker panel in the urine of renal cell carcinoma patients

Date: Oct 16, 2017

Author: separationsNOW

The urinary volatile metabolic profile of patients with renal cell carcinoma was studied by GC/MS to identify a potential specific volatile pattern as a non-invasive strategy to detect the disease.

Read More thumbnail image: Journal Highlight GC-MS metabolomics-based approach for the identification of a potential VOC-biomarker panel in the urine of renal cell carcinoma patients

Influenza A proteins: Individual proteome variations

Date: Oct 15, 2017

Author: Steve Down

Scientists in the US have shown that the proteomes of normal human bronchial epithelial cells are unique for individual people and also respond to infection by the influenza A virus in different ways, findings which could be used to identify people more susceptible to the disease.

Read More thumbnail image: Influenza A proteins Individual proteome variations

Improved detection of cefonicid with 2D HPLC

Date: Oct 15, 2017

Author: Ryan De Vooght-Johnson

Standard HPLC methods for many pharmaceuticals often use non-volatile salts, such as phosphates, which are incompatible with mass spectrometry. Scientists from Zhejiang University and Zhejiang Institute of Food and Drug Control employed two-dimensional HPLC, with phosphate being used for the first column and formate for the second, to detect novel impurities in the antibiotic cefonicid by mass spectrometry.

Read More thumbnail image: Improved detection of cefonicid with 2D HPLC

Small peaks are amplified when stochastic noise is applied

Date: Oct 15, 2017

Author: Ryan De Vooght-Johnson

Small chromatographic peaks that are hidden by baseline noise can be made clearer by employing stochastic resonance, which involves the addition of a limited amount of additional noise. Researchers from China Pharmaceutical University in Nanjing, Nanjing University of Chinese Medicine and Nanjing Changao Pharmaceutical Technology have reported an improved algorithm for the amplification of peaks using stochastic resonance, demonstrating its use with two different examples.

Read More thumbnail image: Small peaks are amplified when stochastic noise is applied

Hydrogen helps hurry harmful dioxins through GC

Date: Oct 15, 2017

Author: Ryan De Vooght-Johnson

Polychlorodibenzo-p-dioxins (PCDDs) and the related polychlorodibenzofurans (PCDFs) are toxic, persistent organic pollutants, but the current official separation methods for the various congeners are lengthy. Scientists from the Institute of Atmospheric Pollution Research in Rome developed a new GC-MS/MS method using hydrogen as the carrier gas rather than helium.

Read More thumbnail image: Hydrogen helps hurry harmful dioxins through GC

Journal Highlight: Statistical model based HPLC analytical method adjustment strategy to adapt to different sets of analytes in complicated samples

Date: Oct 9, 2017

Author: separationsNOW

HPLC data of traditional Chinese medicines acquired with design of experiments methodology were utilised to build retention models for all the analytes and interferences shown in the chromatograms with multivariate statistical modelling methods.

Read More thumbnail image: Journal Highlight Statistical model based HPLC analytical method adjustment strategy to adapt to different sets of analytes in complicated samples

Journal Highlight: Dispersive SPE for the determination of trace organochlorine pesticides in apple juices using reduced graphene oxide coated with ZnO nanocomposites as sorbent

Date: Oct 9, 2017

Author: separationsNOW

Chinese researchers used reduced graphene oxide coated with zinc oxide nanocomposites to conduct the solid-phase extraction of organochlorine pesticides from apple juice followed by gas chromatography with mass spectrometry.

Read More thumbnail image: Journal Highlight Dispersive SPE for the determination of trace organochlorine pesticides in apple juices using reduced graphene oxide coated with ZnO nanocomposites as sorbent
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