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Microwave-assisted extraction technique for preparation of bone tissue

Date: Sep 15, 2015

Author: Ryan De Vooght-Johnson

During a post-mortem examination, determining whether the deceased has ingested a drug can be critical to establishing the cause of death. Usually, this involves analysis of blood, urine, hair, and tissue samples. In circumstances where the body has lain undiscovered for long periods, however, these conventional samples may not be available due to decomposition. In situations where the body has skeletonised, bone tissue becomes a valuable specimen.

Read More thumbnail image: Microwave-assisted extraction technique for preparation of bone tissue

Journal Highlight: Evaluation of hot and cold extraction of bioactive compounds in teas

Date: Sep 14, 2015

Author: separationsNOW

The bioactive compounds of different types of tea in hot and cold infusions were compared using principal components analysis and hierarchical cluster analysis and their activities were determined.

Read More thumbnail image: Journal Highlight Evaluation of hot and cold extraction of bioactive compounds in teas

Monolithic enzyme reactor: Accelerated protein digestion

Date: Sep 7, 2015

Author: Steve Down

An enzyme reactor primed with monolithic material and multiple proteases has been designed for the rapid digestion of proteins with the same efficiency as in-solution digestion but at far faster rates.

Read More thumbnail image: Monolithic enzyme reactor Accelerated protein digestion

OFFGel fractionation: Proteins or peptides?

Date: Aug 24, 2015

Author: Steve Down

The differences revealed by a comparison of OFFGel fractionation of a mouse kidney lysate suggest that the separation of whole proteins is better for shotgun analysis while peptide fractionation is better suited to quantitation by isotope tagging.

Read More thumbnail image: OFFGel fractionation Proteins or peptides

Journal Highlight: Tenax extraction as a simple approach to improve environmental risk assessments

Date: Aug 17, 2015

Author: separationsNOW

The use of Tenax extraction for assessing the bioavailability of hydrophobic organic compounds in sediments is discussed along with a Tenax bioaccumulation model for multiple compounds.

Read More thumbnail image: Journal Highlight Tenax extraction as a simple approach to improve environmental risk assessments

In situ suspended aggregate microextraction: Generic method for organic compounds

Date: Aug 10, 2015

Author: Steve Down

A versatile system for extracting organic compounds from water used supramolecular aggregates prepared in situ to trap the analytes, as illustrated by the analysis or organic UV filter compounds.

Read More thumbnail image: In situ suspended aggregate microextraction Generic method for organic compounds

Wine chlorophenols: Countercurrent chromatography with ionic liquids

Date: Jul 21, 2015

Author: Steve Down

Chlorophenols, which are used as pesticides, have been measured in red wine following extraction by countercurrent chromatography using an ionic liquid to modify one of the solvents.

Read More thumbnail image: Wine chlorophenols Countercurrent chromatography with ionic liquids

Journal Highlight: Comparison of manual and semi-automatic DNA extraction protocols for the barcoding characterization of hematophagous louse flies (Diptera: Hippoboscidae)

Date: Jul 13, 2015

Author: separationsNOW

The number of positive amplifications and the quality of the sequences obtained using four different DNA extraction methods that differ in their cost and time requirements have been compared for the genetic characterization of louse flies.

Read More thumbnail image: Journal Highlight Comparison of manual and semi-automatic DNA extraction protocols for the barcoding characterization of hematophagous louse flies Diptera Hippoboscidae

A spirited defence: Extracting PAHs from liqour

Date: Jul 7, 2015

Author: Steve Down

Polyaromatic hydrocarbons in alcoholic spirits have been extracted by an efficient, rapid and green ultrasound-assisted emulsification microextraction system, the absence of matrix effects in subsequent GC/MS analysis eliminating the need for standard additions during quantification.

Read More thumbnail image: A spirited defence Extracting PAHs from liqour

No fears for tears: One-pot protein extraction

Date: Jun 22, 2015

Author: Steve Down

Scientists in Norway have devised an efficient way to extract proteins from Schirmer tear fluid test strips that is carried out in one vessel and reduces losses, allowing more than 1500 proteins to be detected by LC/MS.

Read More thumbnail image: No fears for tears One-pot protein extraction
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