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Bone protein extraction: Bypassing demineralisation

Date: Mar 9, 2015

Author: Steve Down

Using a novel procedure borrowed from hydroxyapatite chromatography, scientists in the US have succeeded in extracting proteins from human bones in a few hours without initial demineralisation.

Read More thumbnail image: Bone protein extraction Bypassing demineralisation

Proteome coverage: SDS-PAGE versus HPLC

Date: Feb 23, 2015

Author: Steve Down

Two conventional fractionation procedures used in proteome analysis, SDS-PAGE and high-pH RPLC, have been compared at the protein and peptide level, respectively, to see which gives the better protein coverage.

Read More thumbnail image: Proteome coverage SDS-PAGE versus HPLC

Journal Highlight: Methods of biological fluids sample preparation – biogenic amines, methylxanthines, water-soluble vitamins

Date: Feb 16, 2015

Author: separationsNOW

This review provides universal guidance on establishing common procedures across laboratories to facilitate the preparation and analysis of biogenic amines, methylxanthines and water-soluble vitamins in biofluids.

Read More thumbnail image: Journal Highlight Methods of biological fluids sample preparation  biogenic amines methylxanthines water-soluble vitamins

Paper microfluidics: Ion determination and pH measurement

Date: Feb 6, 2015

Author: Steve Down

The use of paper as the sample substrate to make potentiometric measurements of ions in liquids and solids prevents fouling of the electrodes but has some drawbacks due to the paper properties.

Read More thumbnail image: Paper microfluidics Ion determination and pH measurement

Antidepressants in urine: Dispersive microextraction with derivatisation

Date: Jan 26, 2015

Author: Steve Down

Three antidepressants covering two different classes have been extracted from urine by a variation of dispersive liquid-liquid microextraction in which the sample is heated to assist derivatisation and extraction.

Read More thumbnail image: Antidepressants in urine Dispersive microextraction with derivatisation

Journal Highlight: Utilizing a nano-sorbent for the selective solid-phase extraction of vanillic acid prior to its determination by photoluminescence spectroscopy

Date: Jan 19, 2015

Author: separationsNOW

A photoluminescence spectroscopic method was developed for the quantification of vanillic acid in plants after selective extraction using an SPE column packed with nickel–aluminum layered double hydroxide as a nano-sorbent.

Read More thumbnail image: Journal Highlight Utilizing a nano-sorbent for the selective solid-phase extraction of vanillic acid prior to its determination by photoluminescence spectroscopy

A rosy future: Pesticides from rose petals by dispersive SPE

Date: Jan 12, 2015

Author: Steve Down

Pesticide residues in rose petals bound for the perfumery and cosmetics industries have been measured by GC/MS following a modified QuEChERS method based on dispersive solid-phase extraction.

Read More thumbnail image: A rosy future Pesticides from rose petals by dispersive SPE

Urine proteomics: Sample preparation factors

Date: Dec 22, 2014

Author: Steve Down

UK scientists have examined the effects of processing time, temperature and protease inhibitors on the urinary proteome to find that patients’ diseases and the inter-patient variability are more dominant factors.

Read More thumbnail image: Urine proteomics Sample preparation factors

Journal Highlight: Microfluidic sample preparation for elemental analysis in liquid samples using micro X-ray fluorescence spectrometry

Date: Dec 15, 2014

Author: separationsNOW

The suitability of a sample presentation method for small volumes of liquid contained in an XRF-compatible device using a novel prototype chip was compared with that of a commercially produced microfluidic device.

Read More thumbnail image: Journal Highlight Microfluidic sample preparation for elemental analysis in liquid samples using micro X-ray fluorescence spectrometry

Lung cancer breath: Volatiles trapped by monolithic sorptive extraction

Date: Dec 9, 2014

Author: Steve Down

Benzene and related compounds on the breath that might be related to lung cancer have been captured on a new monolithic material for measurement by GC/MS as potential biomarkers.

Read More thumbnail image: Lung cancer breath Volatiles trapped by monolithic sorptive extraction
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