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PALME for acidic drugs: Liquid membrane system

Date: May 11, 2015

Author: Steve Down

Scientists who developed a sample preparation regime called parallel artificial liquid membrane extraction have applied it to the removal of acidic drugs from human plasma.

Read More thumbnail image: PALME for acidic drugs Liquid membrane system

Proteins in urine: Capillary channels in polymer fibres for micro-SPE

Date: Apr 20, 2015

Author: Steve Down

Polymer fibres with inbuilt channels have been used for the extraction of proteins from urine by solid-phase extraction in a rapid and inexpensive preparation for mass spectrometric analysis.

Read More thumbnail image: Proteins in urine Capillary channels in polymer fibres for micro-SPE

Journal Highlight: Cryogenic separation of an oxygen–argon mixture in natural air samples for the determination of isotope and molecular ratios

Date: Apr 13, 2015

Author: separationsNOW

The precise cryogenic separation of oxygen–argon mixtures from natural air samples has been accomplished using a single 5Å (30/60 mesh) molecular sieve column at liquid nitrogen temperature, which is associated with isotopic fractionation.

Read More thumbnail image: Journal Highlight Cryogenic separation of an oxygenargon mixture in natural air samples for the determination of isotope and molecular ratios

Finding phytohormones: Plant purification and pre-treatment regime

Date: Apr 8, 2015

Author: Steve Down

A novel strategy for purifying phytohormones in plant extracts that was devised by Chinese scientists relies on a combination of mixed-mode cation-exchange SPE and liquid-liquid extraction.

Read More thumbnail image: Finding phytohormones Plant purification and pre-treatment regime

Phosphopeptide probe: Loaded SPME fibre as mass spec substrate

Date: Mar 23, 2015

Author: Steve Down

An SPME probe loaded with enriched phosphopeptides was used directly as the source for ambient electrospray ionisation to give excellent sensitivity and selectivity.

Read More thumbnail image: Phosphopeptide probe Loaded SPME fibre as mass spec substrate

Journal Highlight: On-site sampling and sample-preparation approach with a portable sampler based on hollow-fiber/graphene bars for the microextraction of nitrobenzene compounds in lake water

Date: Mar 16, 2015

Author: separationsNOW

Hollow-fiber/graphene bars were used for onsite sampling and sample preparation of four nitrobenzenes in lake water for HPLC analysis.

Read More thumbnail image: Journal Highlight On-site sampling and sample-preparation approach with a portable sampler based on hollow-fibergraphene bars for the microextraction of nitrobenzene compounds in lake water

Bone protein extraction: Bypassing demineralisation

Date: Mar 9, 2015

Author: Steve Down

Using a novel procedure borrowed from hydroxyapatite chromatography, scientists in the US have succeeded in extracting proteins from human bones in a few hours without initial demineralisation.

Read More thumbnail image: Bone protein extraction Bypassing demineralisation

Proteome coverage: SDS-PAGE versus HPLC

Date: Feb 23, 2015

Author: Steve Down

Two conventional fractionation procedures used in proteome analysis, SDS-PAGE and high-pH RPLC, have been compared at the protein and peptide level, respectively, to see which gives the better protein coverage.

Read More thumbnail image: Proteome coverage SDS-PAGE versus HPLC

Journal Highlight: Methods of biological fluids sample preparation – biogenic amines, methylxanthines, water-soluble vitamins

Date: Feb 16, 2015

Author: separationsNOW

This review provides universal guidance on establishing common procedures across laboratories to facilitate the preparation and analysis of biogenic amines, methylxanthines and water-soluble vitamins in biofluids.

Read More thumbnail image: Journal Highlight Methods of biological fluids sample preparation  biogenic amines methylxanthines water-soluble vitamins

Paper microfluidics: Ion determination and pH measurement

Date: Feb 6, 2015

Author: Steve Down

The use of paper as the sample substrate to make potentiometric measurements of ions in liquids and solids prevents fouling of the electrodes but has some drawbacks due to the paper properties.

Read More thumbnail image: Paper microfluidics Ion determination and pH measurement
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