Western Blot Visual Protocol: Phase 3: Membrane Transfer

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  • Published: Mar 1, 2013
  • Source: Novus Biologicals
  • Channels: Sample Preparation
thumbnail image: Western Blot Visual Protocol: Phase 3: Membrane Transfer

Novus Biologicals Visual Protocols: In phase 3 of the western blot procedure, you will learn how to transfer your proteins from the SDS-PAGE gel onto a PVDF or nitrocellulose membrane. Here we remove the gel from the cassette, and stack it in a sandwich comprised of a sponge, filter paper, the gel, membrane, filter paper, and sponge. The negatively charged proteins will then transfer onto the membrane toward the positive current
Additional help can be found in the support section of http://www.novusbio.com.

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