Journal Highlight: Methods of biological fluids sample preparation – biogenic amines, methylxanthines, water-soluble vitamins

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  • Published: Feb 16, 2015
  • Author: separationsNOW
  • Channels: Sample Preparation
thumbnail image: Journal Highlight: Methods of biological fluids sample preparation – biogenic amines, methylxanthines, water-soluble vitamins
This review provides universal guidance on establishing common procedures across laboratories to facilitate the preparation and analysis of biogenic amines, methylxanthines and water-soluble vitamins in biofluids.

Methods of biological fluids sample preparation – biogenic amines, methylxanthines, water-soluble vitamins

Biomedical Chromatography, 2015, 29, 1-20
Joanna Płonka

Abstract: In recent years demands on the amount of information that can be obtained from the analysis of a single sample have increased. For time and economic reasons it is necessary to examine at the same time larger number of compounds, and compounds from different groups. This can best be seen in such areas as clinical analysis. In many diseases, the best results for patients are obtained when treatment fits the individual characteristics of the patient. Dosage monitoring is important at the beginning of therapy and in the full process of treatment. In the treatment of many diseases biogenic amines (dopamine, serotonin) and methylxanthines (theophylline, theobromine, caffeine) play an important role. They are used as drugs separately or in combination with others to support and strengthen the action of other drugs – for example, the combination of caffeine and paracetamol. Vitamin supplementation may be also an integral part of the treatment process. Specification of complete sample preparation parameters for extraction of the above compounds from biological matrices has been reviewed. Particular attention was given to the preparation stage and extraction methods. This review provides universal guidance on establishing common procedures across laboratories to facilitate the preparation and analysis of all discussed compounds.

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