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Journal Highlight: A capillary zone electrophoresis method to detect conformers and dimers of antithrombin in therapeutic preparations

Date: Jul 4, 2016

Author: separationsNOW

A CZE method, based on a neutral polyethylene oxide-coated capillary and a buffer close to physiological conditions, has been devised for the separation of more than eight forms of antithrombin.

Read More thumbnail image: Journal Highlight A capillary zone electrophoresis method to detect conformers and dimers of antithrombin in therapeutic preparations

Deep vein thrombosis and pulmonary embolism: Urinary peptide signs

Date: Jun 16, 2016

Author: Steve Down

There are a number of small peptides in urine that can signal the onset of deep vein thrombosis and pulmonary embolism, say a team of researchers, who hope that their findings will lead to a clinical test for the conditions.

Read More thumbnail image: Deep vein thrombosis and pulmonary embolism Urinary peptide signs

Protein imaging: The power of MALDI

Date: Jun 2, 2016

Author: Steve Down

The power of two different MALDI mass spectrometric techniques for visualising proteins in tissue segments has been demonstrated by US scientists who exploited high-speed scanning and improved spatial resolution capabilities.

Read More thumbnail image: Protein imaging The power of MALDI

Journal Highlight: Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification

Date: May 30, 2016

Author: separationsNOW

Specific CHO host cell protein impurities in non-affinity chromatographic resins used for mAb purification that elute close to mAb products have been identified by proteomic analysis and their chromatographic properties elucidated.

Read More thumbnail image: Journal Highlight Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification

Decompression sickness: Protein biomarkers

Date: May 15, 2016

Author: Steve Down

The onset of decompression sickness in rats leads to a change in the proteome, highlighting proteins which could act as biomarkers of the condition as well as shedding light on the physiological mechanism at play.

Read More thumbnail image: Decompression sickness Protein biomarkers

Journal Highlight: Complexity reduction of clinical samples for routine mass spectrometric analysis

Date: May 2, 2016

Author: separationsNOW

Common protein- and peptide-based fractionation methods for clinical samples are reviewed and illustrated with practical examples, focusing on biomarker validation assays and subsequent routine clinical mass spectrometric analyses.

Read More thumbnail image: Journal Highlight Complexity reduction of clinical samples for routine mass spectrometric analysis

Dioxin exposure: Human protein biomarkers of TCDD

Date: May 1, 2016

Author: Steve Down

Scientists in the Republic of Korea have identified a number of proteins in human hepatocyte cells that are affected by low doses of 2,3,7,8-tetrachloro-p-dioxin and could act as toxicological biomarkers. Some of them were subsequently found in the blood of exposed incineration workers.

Read More thumbnail image: Dioxin exposure Human protein biomarkers of TCDD

Meat management: Tandem MS for species identification

Date: Apr 15, 2016

Author: Steve Down

A wide number of animal species that are raised for human consumption have been identified in an untargeted way by matching peptide tandem mass spectra from shotgun processing to a reference library, which will help to prevent food fraud.

Read More thumbnail image: Meat management Tandem MS for species identification

Journal Highlight: Proteomic profiling of an undefined microbial consortium cultured in fermented dairy manure: Methods development

Date: Apr 4, 2016

Author: separationsNOW

An optimized 2DE workflow has been proposed to investigate the proteome dynamics of mixed microbial consortia that synthesise polyhydroxyalkanoates from fermented dairy manure.

Read More thumbnail image: Journal Highlight Proteomic profiling of an undefined microbial consortium cultured in fermented dairy manure Methods development

Breast cancer biomarkers: Nipple discharge analysis

Date: Apr 2, 2016

Author: Steve Down

Promising biomarkers of invasive breast cancer have been uncovered by scientists in Japan who tapped into nipple discharge, an often ignored biofluid, as a source of locally secreted proteins.

Read More thumbnail image: Breast cancer biomarkers Nipple discharge analysis
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