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Oral disease progression: Protein clues

Date: Nov 2, 2015

Author: Steve Down

The proteomes of oral fluid collected from gingival crevices of healthy people and those with gingivitis and chronic periodontitis have been compared to try to understand the underlying biological processes that take place during oral disease progression.

Read More thumbnail image: Oral disease progression Protein clues

Journal Highlight: Proteomics in neurodegenerative diseases: Methods for obtaining a closer look at the neuronal proteome

Date: Nov 1, 2015

Author: separationsNOW

An overview has been presented of the currently applied state-of-the-art cellular and sub-cellular prefractionation strategies used in the analysis of brain function in normal aging and neurodegenerative, psychiatric, and neurological diseases.

Read More thumbnail image: Journal Highlight Proteomics in neurodegenerative diseases Methods for obtaining a closer look at the neuronal proteome

Early lung cancer biomarker: Linked to squamous metaplasia

Date: Oct 15, 2015

Author: Steve Down

The effects of smoking on the plasma proteome of people with no signs of cancer were examined to see if there were any biomarkers of lung lesions that could be warnings of lung cancer to come, with one protein in particular standing out.

Read More thumbnail image: Early lung cancer biomarker Linked to squamous metaplasia

Protein biomarkers in sweat: Panel of defence proteins established

Date: Oct 1, 2015

Author: Steve Down

The proteins that form the chemical barrier in skin from healthy people have been characterised by sweat analysis, providing the basis for a new starting point for biomarker studies.

Read More thumbnail image: Protein biomarkers in sweat Panel of defence proteins established

Journal Highlight: Interlaboratory study to evaluate the robustness of capillary electrophoresis-mass spectrometry for peptide mapping

Date: Sep 28, 2015

Author: separationsNOW

A collaborative study on the robustness and portability of a CE-MS method for peptide mapping was performed by an international team, consisting of 13 independent laboratories from academia and industry.

Read More thumbnail image: Journal Highlight Interlaboratory study to evaluate the robustness of capillary electrophoresis-mass spectrometry for peptide mapping

HIV-1 and cocaine: Plasma proteome alterations

Date: Sep 15, 2015

Author: Steve Down

The effects of cocaine on the proteome of women infected with HIV-1 have been examined by scientists in Puerto Rico who found that the drug increased levels of apolipoprotein CIII, leading to a greater risk of stroke.

Read More thumbnail image: HIV-1 and cocaine Plasma proteome alterations

Folate and follicle fluid: Proteome analysis in women receiving IVF

Date: Sep 1, 2015

Author: Steve Down

Folic acid supplementation affects the proteome of follicular fluid in women on fertility treatment programmes, which may lead to the discovery of biomarkers for successful IVF or ICSI outcomes.

Read More thumbnail image: Folate and follicle fluid Proteome analysis in women receiving IVF

Journal Highlight: Proteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS. 1. Preparation of more than 4000 native protein maps

Date: Aug 31, 2015

Author: separationsNOW

Soluble proteins of human bronchial smooth muscle cells were separated by nondenaturing micro 2DE and the proteins in 972 gel pieces were measured by LC-MS/MS to give 4323 native protein maps.

Read More thumbnail image: Journal Highlight Proteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MSMS_ 1_ Preparation of more than 4000 native protein maps

Radiation therapy damage: Serum proteins affected

Date: Aug 15, 2015

Author: Steve Down

Radiation therapy for cancer patients affects the serum proteome, increasing levels of proteins associated with acute phase inflammation and reducing levels of lipoproteins and those involved in blood coagulation.

Read More thumbnail image: Radiation therapy damage Serum proteins affected

Journal Highlight: Proteomics beyond trypsin

Date: Aug 3, 2015

Author: separationsNOW

Some of the shortcomings of the nearly exclusive use of trypsin in proteomics are discussed and properties of other proteomics-appropriate proteases are reviewed, with examples of the increased coverage that can be achieved.

Read More thumbnail image: Journal Highlight Proteomics beyond trypsin
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