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Journal Highlight: Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification

Date: May 30, 2016

Author: separationsNOW

Specific CHO host cell protein impurities in non-affinity chromatographic resins used for mAb purification that elute close to mAb products have been identified by proteomic analysis and their chromatographic properties elucidated.

Read More thumbnail image: Journal Highlight Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification

Decompression sickness: Protein biomarkers

Date: May 15, 2016

Author: Steve Down

The onset of decompression sickness in rats leads to a change in the proteome, highlighting proteins which could act as biomarkers of the condition as well as shedding light on the physiological mechanism at play.

Read More thumbnail image: Decompression sickness Protein biomarkers

Journal Highlight: Complexity reduction of clinical samples for routine mass spectrometric analysis

Date: May 2, 2016

Author: separationsNOW

Common protein- and peptide-based fractionation methods for clinical samples are reviewed and illustrated with practical examples, focusing on biomarker validation assays and subsequent routine clinical mass spectrometric analyses.

Read More thumbnail image: Journal Highlight Complexity reduction of clinical samples for routine mass spectrometric analysis

Dioxin exposure: Human protein biomarkers of TCDD

Date: May 1, 2016

Author: Steve Down

Scientists in the Republic of Korea have identified a number of proteins in human hepatocyte cells that are affected by low doses of 2,3,7,8-tetrachloro-p-dioxin and could act as toxicological biomarkers. Some of them were subsequently found in the blood of exposed incineration workers.

Read More thumbnail image: Dioxin exposure Human protein biomarkers of TCDD

Meat management: Tandem MS for species identification

Date: Apr 15, 2016

Author: Steve Down

A wide number of animal species that are raised for human consumption have been identified in an untargeted way by matching peptide tandem mass spectra from shotgun processing to a reference library, which will help to prevent food fraud.

Read More thumbnail image: Meat management Tandem MS for species identification

Journal Highlight: Proteomic profiling of an undefined microbial consortium cultured in fermented dairy manure: Methods development

Date: Apr 4, 2016

Author: separationsNOW

An optimized 2DE workflow has been proposed to investigate the proteome dynamics of mixed microbial consortia that synthesise polyhydroxyalkanoates from fermented dairy manure.

Read More thumbnail image: Journal Highlight Proteomic profiling of an undefined microbial consortium cultured in fermented dairy manure Methods development

Breast cancer biomarkers: Nipple discharge analysis

Date: Apr 2, 2016

Author: Steve Down

Promising biomarkers of invasive breast cancer have been uncovered by scientists in Japan who tapped into nipple discharge, an often ignored biofluid, as a source of locally secreted proteins.

Read More thumbnail image: Breast cancer biomarkers Nipple discharge analysis

Cell and lung transplantation: Protein biomarker for subsequent lung disease

Date: Mar 16, 2016

Author: Steve Down

A plasma protein has been earmarked as a potential marker for the onset of bronchiolitis obliterans syndrome, a life-threatening complication that can follow hematopoietic cell transplantation or lung transplantation.

Read More thumbnail image: Cell and lung transplantation Protein biomarker for subsequent lung disease

Dust mite allergens: Proteomics expands range

Date: Mar 1, 2016

Author: Steve Down

Novel protein allergens from a house dust mite have been revealed by a proteomics study which lends support to the allergen-specific immunotherapeutic approach to treatment.

Read More thumbnail image: Dust mite allergens Proteomics expands range

Journal Highlight: CE–ESI-MS for bottom-up proteomics: Advances in separation, interfacing and applications

Date: Feb 29, 2016

Author: separationsNOW

This review discusses newly developed hyphenation strategies for CE–ESI-MS and their application in bottom-up proteomics, as well as factors in the development of these methods.

Read More thumbnail image: Journal Highlight CEESI-MS for bottom-up proteomics Advances in separation interfacing and applications
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