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Journal Highlight: Benchmarking sample preparation/digestion protocols reveals tube-gel being a fast and repeatable method for quantitative proteomics

Date: Nov 1, 2016

Author: separationsNOW

In the first global label-free quantitative comparison between tube-gel, stacking gel, and basic liquid digestion of proteins mixtures, the tube gel approach proved to be superior.

Read More thumbnail image: Journal Highlight Benchmarking sample preparationdigestion protocols reveals tube-gel being a fast and repeatable method for quantitative proteomics

Atmospheric aerosol proteins: Metaproteomics revelations

Date: Oct 15, 2016

Author: Steve Down

Proteins lurking in the particulates of atmospheric aerosols have been analysed by proteomics techniques, providing a way to assess risks to human health from bacteria and allergens that are present.

Read More thumbnail image: Atmospheric aerosol proteins Metaproteomics revelations

Journal Highlight: Proteomics of hydrophobic samples: Fast, robust and low-cost workflows for clinical approaches

Date: Oct 3, 2016

Author: separationsNOW

In a comparative study, the influence of nine sample preparation workflows and seven different lysis buffers for qualitative and quantitative analysis of the human adipose tissue proteome has been assessed.

Read More thumbnail image: Journal Highlight Proteomics of hydrophobic samples Fast robust and low-cost workflows for clinical approaches

Stress-induced immunosuppression: Proteomics clues

Date: Oct 1, 2016

Author: Steve Down

A proteomics study carried out in China has uncovered proteins that are affected by the onset of immunosuppression brought on by chronic stress, as a first step for elucidating the molecular processes behind the effect.

Read More thumbnail image: Stress-induced immunosuppression Proteomics clues

Red propolis: Anticancer mechanism probed

Date: Sep 15, 2016

Author: Steve Down

The mechanism by which red propolis acts as an anticancer agent can be clarified by the identification of proteins that are differentially expressed in a cancer cell line, say Brazilian researchers.

Read More thumbnail image: Red propolis Anticancer mechanism probed

Journal Highlight: 2D-DIGE-based proteomic analysis of intracoronary versus peripheral arterial blood platelets: Upregulation of platelet activation biomarkers at the culprit site

Date: Sep 9, 2016

Author: separationsNOW

The proteomes of intracoronary and peripheral arterial platelets from STEMI patients were compared to highlight potential platelet biomarkers and drug targets related to the conditions.

Read More thumbnail image: Journal Highlight 2D-DIGE-based proteomic analysis of intracoronary versus peripheral arterial blood platelets Upregulation of platelet activation biomarkers at the culprit site

Growth hormone testing: Gene and protein markers

Date: Sep 6, 2016

Author: Steve Down

Two genes and a protein that appear in blood following consumption of human growth hormone have been proposed as biomarkers of abuse for sports drug testing programmes, offering better sensitivity than insulin-like growth factor-1.

Read More thumbnail image: Growth hormone testing Gene and protein markers

5000 Yeast proteins measured: Cultures with ten carbon sources compared by multiplexed MS

Date: Aug 17, 2016

Author: Steve Down

A modified mass spectrometric protocol with tandem mass tagging has been used to compare protein abundances in yeast cultures grown on ten different carbon sources, providing data for more than 5000 proteins.

Read More thumbnail image: 5000 Yeast proteins measured Cultures with ten carbon sources compared by multiplexed MS

Autoantibody as prostate cancer marker: Enhanced predictive ability in combination with PSA

Date: Aug 3, 2016

Author: Steve Down

A serological proteome analysis of European-African and African-American men has identified one particular natural antibody as a candidate biomarker for prostate cancer that gives high specificity when combined with the conventional PSA test.

Read More thumbnail image: Autoantibody as prostate cancer marker Enhanced predictive ability in combination with PSA

Journal Highlight: State-of-the-art housekeeping proteins for quantitative western blotting: Revisiting the first draft of the human proteome

Date: Aug 1, 2016

Author: separationsNOW

The recently released large-scale, human proteomic database ProteomicsDB, which includes 16,857 LC-tandem-MS data from 27 human tissues, has been examined to propose 20 ubiquitously- and constitutively-expressed, putative internal-reference controls for the quantification of differential protein expressions in Western blotting.

Read More thumbnail image: Journal Highlight State-of-the-art housekeeping proteins for quantitative western blotting Revisiting the first draft of the human proteome
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