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Journal Highlight: Affinity proteomics reveals extensive phosphorylation of the Brassica chromosome axis protein ASY1 and a network of associated proteins at prophase I of meiosis

Date: Jan 29, 2018

Author: separationsNOW

Affinity proteomics has been applied to analyse the proteins that are associated with the meiotic chromosome axis protein, ASY1, in Brassica oleracea anthers and meiocytes.

Read More thumbnail image: Journal Highlight Affinity proteomics reveals extensive phosphorylation of the Brassica chromosome axis protein ASY1 and a network of associated proteins at prophase I of meiosis

Oral squamous cell carcinoma: Dual protein biomarkers

Date: Jan 15, 2018

Author: Steve Down

A combination of two proteins found in saliva can predict oral squamous cell carcinoma and are unaffected by the risk factors human papillomavirus and Epstein-Barr virus, leading to the possibility of a clinical test for the cancer.

Read More thumbnail image: Oral squamous cell carcinoma Dual protein biomarkers

Heart failure: HDL protein signals

Date: Jan 3, 2018

Author: Steve Down

The proteome of high-density lipoprotein is different for survivors of heart failure and those who died, say an international team of researchers who found that a group of 12 proteins gave strong predictive capabilities.

Read More thumbnail image: Heart failure HDL protein signals

Journal Highlight: How many proteins can be identified in a 2-DE gel spot within an analysis of a complex human cancer tissue proteome?

Date: Jan 1, 2018

Author: separationsNOW

Three different mass spectrometric techniques have been compared for the identification of proteins within a single 2-DE gel spot, confirming the necessity of isotopic labeling in large-scale quantification of different protein species in a proteome.

Read More thumbnail image: Journal Highlight How many proteins can be identified in a 2-DE gel spot within an analysis of a complex human cancer tissue proteome

Amyloidosis classification: MALDI mass spectrometric imaging

Date: Dec 15, 2017

Author: Steve Down

Scientists in Germany have used mass spectrometry imaging coupled with ion mobility separation to identify amyloid peptides in a proof-of-concept study that will allow different types of amyloidosis to be classified.

Read More thumbnail image: Amyloidosis classification MALDI mass spectrometric imaging

Monitoring bone fracture healing: Protein signals

Date: Dec 1, 2017

Author: Steve Down

Scientists in the USA have uncovered a suite of proteins in serum that might be able to follow the progression of fracture healing and lead to the development of treatments to accelerate the process.

Read More thumbnail image: Monitoring bone fracture healing Protein signals

Journal Highlight: Tetrazole-based probes for integrated phenotypic screening, affinity-based proteome profiling, and sensitive detection of a cancer biomarker

Date: Nov 27, 2017

Author: separationsNOW

Phenotypic screening of a fully functionalized small-molecule library has been combined with competitive affinity-based proteome profiling to map and functionally characterize the targets of screening hits in drug discovery.

Read More thumbnail image: Journal Highlight Tetrazole-based probes for integrated phenotypic screening affinity-based proteome profiling and sensitive detection of a cancer biomarker

Coffee break: Protein alterations after drinking

Date: Nov 15, 2017

Author: Steve Down

A proteomics study of coffee drinkers in Sweden has uncovered inverse associations with two proteins that have been linked to appetite suppression and a number of diseases, including respiratory disorders, Alzheimer's disease and cancer.

Read More thumbnail image: Coffee break Protein alterations after drinking

E-Cigarettes: As dangerous as cigarettes?

Date: Nov 1, 2017

Author: Steve Down

The safety of e-cigarettes has been thrown into doubt following new research which revealed that it induces high levels of proteins in the lungs that are linked with cystic fibrosis, COPD and bronchitis.

Read More thumbnail image: E-Cigarettes As dangerous as cigarettes

Journal Highlight: A robust protocol for the isolation of cellular proteins from Xanthomonas campestris to analyze the methionine effect in 2D-gel experiments

Date: Oct 30, 2017

Author: separationsNOW

A phenol extraction protocol developed for the purification of bacterial proteins that provides excellent 2D gel separation has been illustrated by examining the effects of methionine on the cellular proteome of Xanthomonas campestris.

Read More thumbnail image: Journal Highlight A robust protocol for the isolation of cellular proteins from Xanthomonas campestris to analyze the methionine effect in 2D-gel experiments
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