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Journal Highlight: A qualitative and quantitative evaluation of the peptide characteristics of microwave- and ultrasound-assisted digestion in discovery and targeted proteomic analyses

Date: Jul 10, 2017

Author: separationsNOW

Three digestion methods, including overnight digestion, microwave-assisted protein enzymatic digestion and high-intensity focused ultrasonic-assisted enzymatic digestion, in trypsin or in trypsin/Lys-C, were compared for discovery and targeted proteomics analysis using the HeLa cell proteome.

Read More thumbnail image: Journal Highlight A qualitative and quantitative evaluation of the peptide characteristics of microwave- and ultrasound-assisted digestion in discovery and targeted proteomic analyses

Buffalo milk: Bioactive peptides

Date: Jul 1, 2017

Author: Steve Down

Peptides derived from the proteins in buffalo milk fat and colostrum by simulated gastrointestinal digestion have a range of bioactive properties, say Indian researchers, highlighting their potential as health product ingredients and neutraceuticals.

Read More thumbnail image: Buffalo milk Bioactive peptides

Alzheimer's biomarkers: Novel metabolites and protein candidates

Date: Jun 15, 2017

Author: Steve Down

Scientists in China have identified a range of potential biomarkers of Alzheimer's disease from a metabolomics and proteomics study of the rat model, providing insight into diagnosis of the condition and candidates for therapeutic targets.

Read More thumbnail image: Alzheimers biomarkers Novel metabolites and protein candidates

Journal Highlight: Proteomics applications in dental derived stem cells

Date: Jun 10, 2017

Author: separationsNOW

An overview has been presented of proteomics studies in the context of stem cell research, focusing on dental-derived stem cell types as well as current challenges and opportunities.

Read More thumbnail image: Journal Highlight Proteomics applications in dental derived stem cells

Gulf War Illness: Proteomics clues to affected biological pathways

Date: Jun 1, 2017

Author: Steve Down

Two complementary proteomics experiments have been carried out on the mouse model of Gulf War Illness to identify modified biological pathways in the central nervous system that might lead to targets for novel therapeutic agents.

Read More thumbnail image: Gulf War Illness Proteomics clues to affected biological pathways

Morphine addiction: Enzyme activity

Date: May 16, 2017

Author: Steve Down

Polish scientists have measured the activities of four enzymes in the brain implicated in the mechanism of morphine addiction in the search for therapeutic targets which do not produce the serious side effects during pain treatment.

Read More thumbnail image: Morphine addiction Enzyme activity

Osteoporosis biomarkers: Serum proteomics

Date: May 1, 2017

Author: Steve Down

A large-scale study of older men in the US has identified a number of proteins in serum that are good candidates for biomarkers of future hip bone loss and fracture by correlation with the bone mineral density measurements.

Read More thumbnail image: Osteoporosis biomarkers Serum proteomics

Journal Highlight: Database of optimized proteomic quantitative methods for human drug disposition-related proteins for applications in physiologically based pharmacokinetic modeling

Date: May 1, 2017

Author: separationsNOW

An open access repository of validated LC-MS/MS MRM methods for quantifying 284 important proteins associated with drug absorption, distribution, metabolism, and excretion has been created.

Read More thumbnail image: Journal Highlight Database of optimized proteomic quantitative methods for human drug disposition-related proteins for applications in physiologically based pharmacokinetic modeling

Milk whey glycoproteins: Interspecies comparison

Date: Apr 15, 2017

Author: Steve Down

A comprehensive examination of the glycoproteins in the milk whey from several mammals by Chinese researchers has identified more than 200 N-glycosylation sites, the majority of which have not been reported previously.

Read More thumbnail image: Milk whey glycoproteins Interspecies comparison

Journal Highlight: Sample clean-up strategies for electrospray ionization mass spectrometry applications in bottom-up proteomics: Trends from 2012–2016

Date: Apr 3, 2017

Author: separationsNOW

Research papers from 2012–2016 on strategies for removal of salts and surfactants prior to electrospray ionization MS analysis in bottom-up proteomics, mostly based on SPE and membrane-based FASP, have been reviewed.

Read More thumbnail image: Journal Highlight Sample clean-up strategies for electrospray ionization mass spectrometry applications in bottom-up proteomics Trends from 20122016
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