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Kawasaki disease: Protein biomarker candidates

Date: Jan 17, 2019

Author: Steve Down

The rare but serious condition known as Kawasaki disease, which mainly affects children under the age of five, produces a modified urinary protein profile that could lead to the development of a diagnostic panel of proteins.

Read More thumbnail image: Kawasaki disease Protein biomarker candidates

Journal Highlight: Alternative digestion approaches improve histone modification mapping by mass spectrometry in clinical samples

Date: Jan 8, 2019

Author: separationsNOW

A novel procedure involving acetone protein precipitation followed by histone enrichment through a C18 StageTip column has been used to obtain histone preparations suitable for various in‐solution digestion protocols and validated on different types of samples.

Read More thumbnail image: Journal Highlight Alternative digestion approaches improve histone modification mapping by mass spectrometry in clinical samples

Preeclampsia proteins: Placental biomarkers

Date: Jan 3, 2019

Author: Steve Down

A proteomics analysis of the placental proteins of pregnant women with preeclampsia has revealed 37 proteins with altered abundances which could be used to develop diagnostic tests or treatments for this disease.

Read More thumbnail image: Preeclampsia proteins Placental biomarkers

Mining the human proteome: Deeper coverage to find missing proteins

Date: Dec 17, 2018

Author: Steve Down

The depth of coverage of the human proteome can be markedly extended by introducing a preliminary alkaline 2D-LC step before analysis by LC/MS with selected reaction monitoring using isotope-labelled standards or shotgun LC/MS/MS analysis.

Read More thumbnail image: Mining the human proteome Deeper coverage to find missing proteins

Paediatric kidney transplant rejection: Peptide biomarkers

Date: Dec 1, 2018

Author: Steve Down

Kidney transplant rejection in children, specifically chronic active antibody-mediated rejection (cAMBR), can be predicted by a panel of urinary peptides, many derived from collagen species, a development that could indicate those patients who would benefit from increased immunosuppressive treatment.

Read More thumbnail image: Paediatric kidney transplant rejection Peptide biomarkers

Journal Highlight: The art of validating quantitative proteomics data

Date: Nov 26, 2018

Author: separationsNOW

The pros and cons of Western blotting as a validation approach for quantitative proteomics data have been discussed using examples from the authors' own published work.

Read More thumbnail image: Journal Highlight The art of validating quantitative proteomics data

Acupuncture for vascular dementia: Proteins implicated

Date: Nov 15, 2018

Author: Steve Down

The molecular mechanisms behind the improvement of vascular dementia initiated by acupuncture have been investigated by Chinese scientists who used proteomics to indicate that proteins involved in oxidative stress, apoptosis and synaptic function were affected.

Read More thumbnail image: Acupuncture for vascular dementia Proteins implicated

Meat adulteration: Substitute meats and allergens

Date: Nov 2, 2018

Author: Steve Down

The adulteration of meat and meat products by other types of meat or allergenic foods such as egg white, soy and milk has been accomplished using a stable-isotope-labelled quantification strategy by scientists in Poland.

Read More thumbnail image: Meat adulteration Substitute meats and allergens

Journal Highlight: Updates of the in‐gel digestion method for protein analysis by mass spectrometry

Date: Oct 29, 2018

Author: separationsNOW

The in-gel digestion process for the analysis of proteins by LC/MS has been updated by using alternative reducing and alkylating agents and tryptic digestion buffers to give superior protein identification and sequence coverage with reduced side reactions and handling time.

Read More thumbnail image: Journal Highlight Updates of the ingel digestion method for protein analysis by mass spectrometry

Journal Highlight: Structural elucidation of trace components combining GC/MS, GC/IR, DFT‐calculation and synthesis ‐ Salinilactones, unprecedented bicyclic lactones from Salinispora bacteria

Date: Oct 15, 2018

Author: separationsNOW

Salinilactones displaying an unusual bicyclic [3.1.0]‐lactone skeleton that are structurally closely related to the A‐factor class of compounds have been identified in marine Salinispora bacteria using GC/MS, GC/IR, DFT‐calculation and synthesis.

Read More thumbnail image: Journal Highlight Structural elucidation of trace components combining GCMS GCIR DFTcalculation and synthesis  Salinilactones unprecedented bicyclic lactones from Salinispora bacteria
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