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Sample preparation in proteomics: Method bias

Date: May 15, 2018

Author: Steve Down

A comparison of four commonly used proteomics sample preparation methods for head and neck tissues has revealed that there is no universal method, each having their own deficiencies and advantages.

Read More thumbnail image: Sample preparation in proteomics Method bias

Postmortem interval: Forensic proteomic pointers

Date: May 1, 2018

Author: Steve Down

A proteomics study of decaying bones has thrown up a number of proteins that might prove to be valuable markers of the skeletal postmortem interval as an aid to forensic scientists.

Read More thumbnail image: Postmortem interval Forensic proteomic pointers

Journal Highlight: Cell‐free DNA blood collection tubes are appropriate for clinical proteomics: A demonstration in colorectal cancer

Date: Apr 30, 2018

Author: separationsNOW

A proteomics study of colorectal cancer patients has shown that optimized blood collection tubes are appropriate for low‐abundant plasma protein analysis and can be used with confidence for clinical proteomics.

Read More thumbnail image: Journal Highlight Cellfree DNA blood collection tubes are appropriate for clinical proteomics A demonstration in colorectal cancer

Anton Checkov: Cause of death questioned

Date: Apr 15, 2018

Author: Steve Down

Proteome analysis of brown stains on archived personal items of Anton Checkov has yielded proteins related to Mycobacterium tuberculosis, confirming that he had tuberculosis at time of death, but another protein suggests that there may have been a different cause of death.

Read More thumbnail image: Anton Checkov Cause of death questioned

Journal Highlight: 'One‐pot' sample processing method for proteome‐wide analysis of microbial cells and spores

Date: Apr 3, 2018

Author: separationsNOW

A simple 'one‐pot' method for the proteome analysis of bacterial spores has been demonstrated for Bacillus subtilis, Bacillus cereus and Peptoclostridium difficile, yielding to the identification of >1000 across all spore layers of the three organisms.

Read More thumbnail image: Journal Highlight Onepot sample processing method for proteomewide analysis of microbial cells and spores

Asthma at work: Proteomics search for biomarkers

Date: Apr 1, 2018

Author: Steve Down

Nasal protein profiling has been used to measure the responses of people exposed to airborne allergens like isocyanates and welding fumes, leading to several candidate biomarkers for work-related asthma.

Read More thumbnail image: Asthma at work Proteomics search for biomarkers

Mortality biomarkers: Peptides and proteins

Date: Mar 15, 2018

Author: Steve Down

A large-scale proteomics study on older men has identified a number of peptides and proteins that are associated with five-year mortality, most of them arising from non-cancer deaths and implicating a number of biological pathways, especially inflammation.

Read More thumbnail image: Mortality biomarkers Peptides and proteins

Journal Highlight: Comparative evaluation of five protocols for protein extraction from stony corals (Scleractinia) for proteomics

Date: Mar 5, 2018

Author: separationsNOW

TRIzol, phenol-based extraction, trichloroacetic acid-acetone, glass bead-assisted extraction and a commercially available kit have been compared for the extraction of proteins from stony corals for analysis by 2-DE and MALDI-TOF-MS.

Read More thumbnail image: Journal Highlight Comparative evaluation of five protocols for protein extraction from stony corals Scleractinia for proteomics

Breast cancer in young women: Biomarkers from breast milk

Date: Mar 1, 2018

Author: Steve Down

A preliminary proteomics study of breast milk in younger women collected within two years of them being diagnosed with breast cancer has revealed a number of dysregulated proteins as potential candidates for early detection of the disease.

Read More thumbnail image: Breast cancer in young women Biomarkers from breast milk

Canine saliva: Inter-breed proteomics

Date: Feb 15, 2018

Author: Steve Down

The proteome of dog saliva differs between breeds and between individual animals of the same breed, say scientists in the UK, who compared differently sized breeds for the first time.

Read More thumbnail image: Canine saliva Inter-breed proteomics
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