Journal Highlight: Sample clean-up strategies for electrospray ionization mass spectrometry applications in bottom-up proteomics: Trends from 2012–2016

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  • Published: Apr 3, 2017
  • Author: separationsNOW
  • Channels: Proteomics & Genomics
thumbnail image: Journal Highlight: Sample clean-up strategies for electrospray ionization mass spectrometry applications in bottom-up proteomics: Trends from 2012–2016

Research papers from 2012–2016 on strategies for removal of salts and surfactants prior to electrospray ionization MS analysis in bottom-up proteomics, mostly based on SPE and membrane-based FASP, have been reviewed.

Sample clean-up strategies for electrospray ionization mass spectrometry applications in bottom-up proteomics: Trends from 2012–2016

Proteomics, 2017, online
Ria Marni Tubaon, Paul R. Haddad and Joselito P. Quirino

Abstract: Bottom-up proteomics is a mass spectrometric (MS)-based approach for the characterization of peptides obtained from in-solution protein digestion. MS is favoured over other methods for peptide and protein analysis because of its better sensitivity and high throughput. Inorganic ions and surfactants present in the sample or produced during tryptic digestion are detrimental in MS analysis and affect the proteome data, thus sample preparation for removal of these unwanted components has become essential. Here, we review 48 research papers on strategies for removal of salts and surfactants (in particular, sodium dodecyl sulfate, SDS) prior to electrospray ionization (ESI)-MS analysis in bottom-up proteomics from 2012–2016. The strategies were mostly based on solid-phase extraction (SPE) and membrane-based filter-aided sample preparation (FASP) for salt and SDS removal, respectively. Some known limitations of SPE and FASP procedures are that they can be time consuming, laborious, and require the use of organic solvents before a concentrated extract suitable for analysis is obtained. The development of faster analytical methods by reducing the sample preparation time and thereby, increasing sample throughput, and in a solvent-less and membrane-less operation, is a significant contribution to proteome research.

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