Journal Highlight: CE–ESI-MS for bottom-up proteomics: Advances in separation, interfacing and applications

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  • Published: Feb 29, 2016
  • Author: separationsNOW
  • Channels: Proteomics & Genomics
thumbnail image: Journal Highlight: CE–ESI-MS for bottom-up proteomics: Advances in separation, interfacing and applications
This review discusses newly developed hyphenation strategies for CE–ESI-MS and their application in bottom-up proteomics, as well as factors in the development of these methods.

CE–ESI-MS for bottom-up proteomics: Advances in separation, interfacing and applications

Mass Spectrometry Reviews, 2016, 35, 259-271
Anthonius A.M. Heemskerk, André M. Deelder and Oleg A. Mayboroda

Abstract: With the development of more sensitive hyphenation strategies for capillary electrophoresis–electrospray-mass spectrometry the technique has reemerged as technique with high separation power combined with high sensitivity in the analysis of peptides and protein digests. This review will discuss the newly developed hyphenation strategies for CE–ESI-MS and their application in bottom-up proteomics as well as the applications in the same time span, 2009 to present, using co-axial sheath liquid. Subsequently all separate aspects in the development of a CE–ESI-MS method for bottom-up proteomics shall be discussed, highlighting certain applications and discussing pros and cons of the various choices. The separation of peptides in a capillary electrophoresis system is discussed including the great potential for modeling of this migration of peptides due to the simple electrophoretic separation process. Furthermore, the technical aspects of method development are discussed, namely; background electrolyte choice, coating of the separation capillary and chosen loading method. Finally, conclusions and an outlook on future developments in the field of bottom-up proteomics by CE–ESI-MS will be provided.

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