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Journal Highlight: Identification of metabolites of gardenin A in rats by combination of HPLC with linear ion trap–Orbitrap MS based on multiple data processing techniques

Date: Feb 16, 2015

Author: separationsNOW

By applying various data processing techniques to HPLC-MS data, Chinese researchers have managed to profile the metabolic fate of a health-promoting flavonoid known as gardenin A in rats.

Read More thumbnail image: Journal Highlight Identification of metabolites of gardenin A in rats by combination of HPLC with linear ion trapOrbitrap MS based on multiple data processing techniques

Journal Highlight: PLS-DA for compositional data with application to metabolomics

Date: Feb 9, 2015

Author: separationsNOW

Researchers from Austria and the Czech Republic have demonstrated how partial least squares discriminant analysis can be applied to compositional data in metabolomics.

Read More thumbnail image: Journal Highlight PLS-DA for compositional data with application to metabolomics

What a transformation: Replacing time with a physical property in electropherograms

Date: Feb 2, 2015

Author: Jon Evans

After showing that the process of converting a standard time-based electropherogram into one that displays the distribution of a physical property is more complicated than it first appears, French researchers developed a method for doing just that.

Read More thumbnail image: What a transformation Replacing time with a physical property in electropherograms

Journal Highlight: ProteoAnnotator – Open source proteogenomics annotation software supporting PSI standards

Date: Jan 19, 2015

Author: separationsNOW

British researchers have developed open-source annotation software for proteogenomics, called ProteoAnnotator, which incorporates a graphical user interface and implements the Proteomics Standards Initiative mzIdentML standard.

Read More thumbnail image: Journal Highlight ProteoAnnotator  Open source proteogenomics annotation software supporting PSI standards

Journal Highlight: CE fingerprinting coupled with chemometrics to evaluate the quality consistency and predict the antioxidant activity of Sanhuang tablet as part of its quality control

Date: Jan 12, 2015

Author: separationsNOW

Chinese researchers have constructed a capillary electrophoresis fingerprint for Sanhuang tablet, a Chinese traditional patent medicine, and used it to determine the quality consistency of tablets from various manufacturers.

Read More thumbnail image: Journal Highlight CE fingerprinting coupled with chemometrics to evaluate the quality consistency and predict the antioxidant activity of Sanhuang tablet as part of its quality control

Resolving curves in two dimensions: Analyte quantification in 2D liquid chromatography

Date: Jan 5, 2015

Author: Jon Evans

Using detectors at the end of each column and multivariate curve resolution, US chemists have come up with a way to make 2D liquid chromatography more accurate at determining analyte concentrations.

Read More thumbnail image: Resolving curves in two dimensions Analyte quantification in 2D liquid chromatography

Journal Highlight: Comparative study of the alignment method on experimental and simulated chromatographic data

Date: Dec 15, 2014

Author: separationsNOW

This paper provides an up-to-date review of the alignment algorithms, methods and improvements used in chromatography, with a particular emphasis on one-dimensional signals.

Read More thumbnail image: Journal Highlight Comparative study of the alignment method on experimental and simulated chromatographic data

Journal Highlight: Identification of phosphopeptides with unknown cleavage specificity by a de novo sequencing assisted database search strategy

Date: Dec 8, 2014

Author: separationsNOW

Chinese scientists have investigated the performance of a de novo sequencing assisted database search strategy for the identification of phosphopeptides in protein digests.

Read More thumbnail image: Journal Highlight Identification of phosphopeptides with unknown cleavage specificity by a de novo sequencing assisted database search strategy

Blast the fat: Novel software tool for producing MS/MS spectral libraries of lipids

Date: Dec 2, 2014

Author: Jon Evans

By making it easier to create tandem mass spectral libraries, a novel software tool should greatly speed up lipid analysis.

Read More thumbnail image: Blast the fat Novel software tool for producing MSMS spectral libraries of lipids

Journal Highlight: A collection of open source applications for mass spectrometry data mining

Date: Nov 17, 2014

Author: separationsNOW

This paper details several freely-available bioinformatics applications for identifying and quantifying phosphoproteome components in mass spectrometry data.

Read More thumbnail image: Journal Highlight A collection of open source applications for mass spectrometry data mining
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