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Journal Highlight: Investigation of protein selectivity in multimodal chromatography using in silico designed Fab fragment variants

Date: Oct 26, 2015

Author: separationsNOW

Scientists from Denmark and the US have designed a set of antibody fragments in silico to examine the relationship between protein surface properties and selectivity in multimodal chromatographic systems.

Read More thumbnail image: Journal Highlight Investigation of protein selectivity in multimodal chromatography using in silico designed Fab fragment variants

How to spot a tooled-up protein: Analyzing protein-based therapeutics with IEC

Date: Oct 12, 2015

Author: Jon Evans

Using ion-exchange chromatography, US scientists have developed a method for analyzing the structure of protein-based therapeutics, allowing them to make sure they’re properly tooled-up.

Read More thumbnail image: How to spot a tooled-up protein Analyzing protein-based therapeutics with IEC

Journal Highlight: Isolation and purification of monosialotetrahexosylgangliosides from pig brain by extraction and LC

Date: Oct 5, 2015

Author: separationsNOW

Using several forms of chromatography, including ion exchange chromatography, Chinese scientists have developed a novel method for isolating and purifying a glycosphingolipid with medical properties from pig brains.

Read More thumbnail image: Journal Highlight Isolation and purification of monosialotetrahexosylgangliosides from pig brain by extraction and LC

Journal Highlight: Synergistic action of laccases from Trametes hirsuta Bm2 improves decolourization of indigo carmine

Date: Sep 28, 2015

Author: separationsNOW

Scientists from Mexico and Cuba used anion exchange chromatography to isolate fungal laccase isoenzymes that were able to discolor indigo dye.

Read More thumbnail image: Journal Highlight Synergistic action of laccases from Trametes hirsuta Bm2 improves decolourization of indigo carmine

Sweet taste of honey: Analyzing the sugar molecules in nectar and honeydew

Date: Sep 14, 2015

Author: Jon Evans

Nectar and honeydew are both sugar-rich substances collected by bees to produce honey, but a new ion chromatography method has revealed that they each posses a very different mix of sugar molecules.

Read More thumbnail image: Sweet taste of honey Analyzing the sugar molecules in nectar and honeydew

Journal Highlight: Purification and characterization of organic solvent stable serine alkaline protease from newly isolated Bacillus circulans M34

Date: Sep 7, 2015

Author: separationsNOW

Using anion exchange chromatography, Turkish chemists have purified a novel protease from a newly isolated strain of bacteria.

Read More thumbnail image: Journal Highlight Purification and characterization of organic solvent stable serine alkaline protease from newly isolated Bacillus circulans M34

Journal Highlight: Structural characteristics of pineapple pulp polysaccharides and their antitumor cell proliferation activities

Date: Aug 24, 2015

Author: separationsNOW

Using anion exchange chromatography and NMR spectroscopy, Chinese scientists have analyzed the structure of bioactive polysaccharides found in pineapples.

Read More thumbnail image: Journal Highlight Structural characteristics of pineapple pulp polysaccharides and their antitumor cell proliferation activities

High-speed protein separation in a bottle: IEC with capillary-channeled polymers

Date: Aug 10, 2015

Author: Jon Evans

By coating an amine-rich polymer on to a special kind of polymer fiber, US chemists have come up with a novel way to conduct high-throughput protein separations.

Read More thumbnail image: High-speed protein separation in a bottle IEC with capillary-channeled polymers

Journal Highlight: Phosphoproteomics of the goat milk fat globule membrane: New insights into lipid droplet secretion from the mammary epithelial cell

Date: Aug 3, 2015

Author: separationsNOW

Scientists from France and Tunisia have used cation-exchange chromatography to investigate whether the proteins in the goat milk fat globule membrane are phosphorylated.

Read More thumbnail image: Journal Highlight Phosphoproteomics of the goat milk fat globule membrane New insights into lipid droplet secretion from the mammary epithelial cell

Journal Highlight: Dysregulation of inter-photoreceptor retinoid-binding protein after induced Müller cell disruption

Date: Jul 27, 2015

Author: separationsNOW

Scientists in Australia have used ion-exchange chromatography and hydrophobic interaction chromatography to isolate a protein that may be involved in certain retinal diseases.

Read More thumbnail image: Journal Highlight Dysregulation of inter-photoreceptor retinoid-binding protein after induced Muller cell disruption
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