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IEC makes light work: Of characterizing proteins

Date: Jun 11, 2018

Author: Jon Evans

For characterizing certain proteins, scientists in Israel have shown that multi-angle light scattering is better combined with ion-exchange chromatography rather than the more conventional size-exclusion chromatography.

Read More thumbnail image: IEC makes light work Of characterizing proteins

Journal Highlight: Determination of D‐myo‐inositol phosphates in 'activated' raw almonds using anion exchange chromatography coupled with tandem mass spectrometry

Date: Jun 4, 2018

Author: separationsNOW

Food scientists in the US used anion-exchange chromatography and tandem mass spectrometry to analyze the phosphates in 'activated' almonds, which have been soaked in water for 12–24 hours.

Read More thumbnail image: Journal Highlight Determination of Dmyoinositol phosphates in activated raw almonds using anion exchange chromatography coupled with tandem mass spectrometry

Extracellular activities: Isolating extracellular vesicles with anion-exchange chromatography

Date: May 14, 2018

Author: Jon Evans

Researchers from the pharmaceutical giant AstraZeneca have shown that anion-exchange chromatography offers a quick and effective way of isolating extracellular vesicles, which show promise for drug delivery, from cell cultures.

Read More thumbnail image: Extracellular activities Isolating extracellular vesicles with anion-exchange chromatography

Journal Highlight: Extraction, isolation, and concentration of calprotectin antigen from granulocytes

Date: May 7, 2018

Author: separationsNOW

Scientists from Sweden and Norway used ion-exchange chromatography to purify calprotectin, a promising biomarker for inflammatory bowel disease, from granulocyte extracts.

Read More thumbnail image: Journal Highlight Extraction isolation and concentration of calprotectin antigen from granulocytes

Thinking inside the box: A novel alternative to preparative chromatography

Date: Apr 9, 2018

Author: Jon Evans

Canadian researchers show that a box filled with ion-exchange particles offers a more efficient way to purify protein-based biopharmaceuticals than conventional preparative chromatography.

Read More thumbnail image: Thinking inside the box A novel alternative to preparative chromatography

Journal Highlight: Plasma metabolome analysis of patients with major depressive disorder

Date: Apr 2, 2018

Author: separationsNOW

Japanese researchers used ion chromatography to measure levels of the metabolite phosphoethanolamine in blood plasma, as a candidate biomarker of major depressive disorder.

Read More thumbnail image: Journal Highlight Plasma metabolome analysis of patients with major depressive disorder

Journal Highlight: Separation and determination of polyurethane amine catalysts in polyether polyols by using UHPLC–Q‐TOF‐MS on a reversed‐phase/cation‐exchange mixed‐mode column

Date: Mar 26, 2018

Author: separationsNOW

Chinese scientists have used a reversed‐phase/cation‐exchange mixed‐mode chromatography column, with mass spectrometry, to analyze polyurethane amine catalysts in polyether polyols.

Read More thumbnail image: Journal Highlight Separation and determination of polyurethane amine catalysts in polyether polyols by using UHPLCQTOFMS on a reversedphasecationexchange mixedmode column

The joy of SAXS: When combined with ion-exchange chromatography

Date: Mar 12, 2018

Author: Jon Evans

By taking advantage of a micro-ionization chamber, a Japanese scientist has been able to combine small-angle X-ray scattering with ion-exchange chromatography to produce a new method for characterizing proteins.

Read More thumbnail image: The joy of SAXS When combined with ion-exchange chromatography

Journal Highlight: Glycosyltransferase cascades made fit for chemical production: Integrated biocatalytic process for the natural polyphenol C-glucoside nothofagin

Date: Mar 5, 2018

Author: separationsNOW

Austrian scientists used anion-exchange chromatography to capture and purify a glycosylated biomolecule produced by a novel glycosyltransferase reaction cascade.

Read More thumbnail image: Journal Highlight Glycosyltransferase cascades made fit for chemical production Integrated biocatalytic process for the natural polyphenol C-glucoside nothofagin

Journal Highlight: Simple determination of betaine, L-carnitine and choline in human urine using self-packed column and column-switching ion chromatography with nonsuppressed conductivity detection

Date: Feb 26, 2018

Author: separationsNOW

Chemists in China have used column-switching ion chromatography to analyze the quaternary ammonium salts betaine, L-carnitine and choline in human urine.

Read More thumbnail image: Journal Highlight Simple determination of betaine L-carnitine and choline in human urine using self-packed column and column-switching ion chromatography with nonsuppressed conductivity detection
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