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Stress-free removals: Removing SDS from proteins by cation-exchange chromatography

Date: Jul 9, 2018

Author: Jon Evans

Scientists from Singapore have developed a new process, based on cation exchange chromatography, for removing the surfactant sodium dodecyl sulfate from proteins prior to analysis by mass spectrometry.

Read More thumbnail image: Stress-free removals Removing SDS from proteins by cation-exchange chromatography

Journal Highlight: Systematic interpolation method predicts antibody monomer‐dimer separation by gradient elution chromatography at high protein loads

Date: Jul 4, 2018

Author: separationsNOW

Chemical engineers in the US have adapted an empirical interpolation method to predict highly overloaded multicomponent elution behavior in cation-exchange chromatography.

Read More thumbnail image: Journal Highlight Systematic interpolation method predicts antibody monomerdimer separation by gradient elution chromatography at high protein loads

Journal Highlight: Hydrochemistry of Lake Rara: A high mountain lake in western Nepal

Date: Jun 25, 2018

Author: separationsNOW

Scientists from Nepal have used ion chromatography to analyze the ions in water samples from Lake Rara, a high mountain lake in western Nepal.

Read More thumbnail image: Journal Highlight Hydrochemistry of Lake Rara A high mountain lake in western Nepal

IEC makes light work: Of characterizing proteins

Date: Jun 11, 2018

Author: Jon Evans

For characterizing certain proteins, scientists in Israel have shown that multi-angle light scattering is better combined with ion-exchange chromatography rather than the more conventional size-exclusion chromatography.

Read More thumbnail image: IEC makes light work Of characterizing proteins

Journal Highlight: Determination of D‐myo‐inositol phosphates in 'activated' raw almonds using anion exchange chromatography coupled with tandem mass spectrometry

Date: Jun 4, 2018

Author: separationsNOW

Food scientists in the US used anion-exchange chromatography and tandem mass spectrometry to analyze the phosphates in 'activated' almonds, which have been soaked in water for 12–24 hours.

Read More thumbnail image: Journal Highlight Determination of Dmyoinositol phosphates in activated raw almonds using anion exchange chromatography coupled with tandem mass spectrometry

Extracellular activities: Isolating extracellular vesicles with anion-exchange chromatography

Date: May 14, 2018

Author: Jon Evans

Researchers from the pharmaceutical giant AstraZeneca have shown that anion-exchange chromatography offers a quick and effective way of isolating extracellular vesicles, which show promise for drug delivery, from cell cultures.

Read More thumbnail image: Extracellular activities Isolating extracellular vesicles with anion-exchange chromatography

Journal Highlight: Extraction, isolation, and concentration of calprotectin antigen from granulocytes

Date: May 7, 2018

Author: separationsNOW

Scientists from Sweden and Norway used ion-exchange chromatography to purify calprotectin, a promising biomarker for inflammatory bowel disease, from granulocyte extracts.

Read More thumbnail image: Journal Highlight Extraction isolation and concentration of calprotectin antigen from granulocytes

Thinking inside the box: A novel alternative to preparative chromatography

Date: Apr 9, 2018

Author: Jon Evans

Canadian researchers show that a box filled with ion-exchange particles offers a more efficient way to purify protein-based biopharmaceuticals than conventional preparative chromatography.

Read More thumbnail image: Thinking inside the box A novel alternative to preparative chromatography

Journal Highlight: Plasma metabolome analysis of patients with major depressive disorder

Date: Apr 2, 2018

Author: separationsNOW

Japanese researchers used ion chromatography to measure levels of the metabolite phosphoethanolamine in blood plasma, as a candidate biomarker of major depressive disorder.

Read More thumbnail image: Journal Highlight Plasma metabolome analysis of patients with major depressive disorder

Journal Highlight: Separation and determination of polyurethane amine catalysts in polyether polyols by using UHPLC–Q‐TOF‐MS on a reversed‐phase/cation‐exchange mixed‐mode column

Date: Mar 26, 2018

Author: separationsNOW

Chinese scientists have used a reversed‐phase/cation‐exchange mixed‐mode chromatography column, with mass spectrometry, to analyze polyurethane amine catalysts in polyether polyols.

Read More thumbnail image: Journal Highlight Separation and determination of polyurethane amine catalysts in polyether polyols by using UHPLCQTOFMS on a reversedphasecationexchange mixedmode column
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