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Cisplatin in tissues: Liver and kidney distribution by LC/MS

Date: Jul 13, 2015

Author: Steve Down

A novel LC/MS/MS method for measuring the allocation of the anticancer drug cisplatin between rat liver and kidney will clarify the relationship between its toxicity, dosage and distribution.

Read More thumbnail image: Cisplatin in tissues Liver and kidney distribution by LCMS

Phytoplankton pigments: HPLC separation of chlorophylls and carotenoids

Date: Jul 1, 2015

Author: Steve Down

A novel HPLC method for analysing structurally related chlorophylls and carotenoids in phytoplankton in one run will help to pin down which species of red and green microalgae are present in ocean environments and how they react to changing light conditions.

Read More thumbnail image: Phytoplankton pigments HPLC separation of chlorophylls and carotenoids

Journal Highlight: D-Phenothrin-induced oxidative DNA damage in rat liver and kidney determined by HPLC-ECD/DAD

Date: Jun 22, 2015

Author: separationsNOW

The risk of genotoxicity of the pyrethroid insecticide D-phenothrin was assessed by measuring the oxidative stress it causes in rat liver and kidney.

Read More thumbnail image: Journal Highlight D-Phenothrin-induced oxidative DNA damage in rat liver and kidney determined by HPLC-ECDDAD

HPLC going green: Generic replacement of acetonitrile with acetone

Date: Jun 18, 2015

Author: Steve Down

Acetone has been explored as a greener mobile phase solvent than acetonitrile for HPLC separations, illustrated by the analysis of a plant extract using a corona charged aerosol detector.

Read More thumbnail image: HPLC going green Generic replacement of acetonitrile with acetone

Vulcanisation accelerator biomonitoring: Urinary levels of 2-mercaptobenzothiazole by LC/MS

Date: Jun 1, 2015

Author: Steve Down

A common rubber additive, 2-mercaptobenzothiazole, which has been used as a rubber vulcaniser for decades, has been measured in human urine by an LC/MS method as part of a German biomonitoring project.

Read More thumbnail image: Vulcanisation accelerator biomonitoring Urinary levels of 2-mercaptobenzothiazole by LCMS

Journal Highlight: Refolding and simultaneous purification of recombinant human proinsulin from inclusion bodies on protein-folding liquid-chromatography columns

Date: May 25, 2015

Author: separationsNOW

Refolding with simultaneous purification of recombinant human proinsulin from inclusion bodies from Escherichia coli were investigated using the surface of stationary phases in IMAC and high-performance SEC.

Read More thumbnail image: Journal Highlight Refolding and simultaneous purification of recombinant human proinsulin from inclusion bodies on protein-folding liquid-chromatography columns

Sweet pollutants: Sweeteners in rivers by LC/MS

Date: May 18, 2015

Author: Steve Down

Eight sweeteners have been measured in environmental water by an LC/MS method, including stevioside and glycyrrhizic acid for the first time, showing that they are not completely removed by wastewater treatment plants.

Read More thumbnail image: Sweet pollutants Sweeteners in rivers by LCMS

Cytochrome P450 A12 phenotyping: HPLC of salivary caffeine metabolites

Date: May 4, 2015

Author: Steve Down

The activity of the enzyme cytochrome P450 1A2 has been determined in humans using a newly developed HPLC method which measures the conversion of caffeine to paraxanthine in saliva.

Read More thumbnail image: Cytochrome P450 A12 phenotyping HPLC of salivary caffeine metabolites

Journal Highlight: Improvements in phenotype studies of hemoglobin disorders brought by advances in reversed-phase chromatography of globin chains

Date: Apr 20, 2015

Author: separationsNOW

RP-HPLC has been used to unravel unclear phenotypes which are modified by the presence of an additional variant not detected by the conventional methods, and help to characterize rare mutants.

Read More thumbnail image: Journal Highlight Improvements in phenotype studies of hemoglobin disorders brought by advances in reversed-phase chromatography of globin chains

Green HPLC: New methods for multicomponent pharmaceuticals

Date: Apr 13, 2015

Author: Steve Down

Scientists in Egypt have applied green principles to the HPLC analysis of four pharmaceutical formulations containing multiple active ingredients, to illustrate that conventional procedures can be replaced with no detrimental effects on method performance.

Read More thumbnail image: Green HPLC New methods for multicomponent pharmaceuticals
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