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Journal Highlight: Refolding and simultaneous purification of recombinant human proinsulin from inclusion bodies on protein-folding liquid-chromatography columns

Date: May 25, 2015

Author: separationsNOW

Refolding with simultaneous purification of recombinant human proinsulin from inclusion bodies from Escherichia coli were investigated using the surface of stationary phases in IMAC and high-performance SEC.

Read More thumbnail image: Journal Highlight Refolding and simultaneous purification of recombinant human proinsulin from inclusion bodies on protein-folding liquid-chromatography columns

Sweet pollutants: Sweeteners in rivers by LC/MS

Date: May 18, 2015

Author: Steve Down

Eight sweeteners have been measured in environmental water by an LC/MS method, including stevioside and glycyrrhizic acid for the first time, showing that they are not completely removed by wastewater treatment plants.

Read More thumbnail image: Sweet pollutants Sweeteners in rivers by LCMS

Cytochrome P450 A12 phenotyping: HPLC of salivary caffeine metabolites

Date: May 4, 2015

Author: Steve Down

The activity of the enzyme cytochrome P450 1A2 has been determined in humans using a newly developed HPLC method which measures the conversion of caffeine to paraxanthine in saliva.

Read More thumbnail image: Cytochrome P450 A12 phenotyping HPLC of salivary caffeine metabolites

Journal Highlight: Improvements in phenotype studies of hemoglobin disorders brought by advances in reversed-phase chromatography of globin chains

Date: Apr 20, 2015

Author: separationsNOW

RP-HPLC has been used to unravel unclear phenotypes which are modified by the presence of an additional variant not detected by the conventional methods, and help to characterize rare mutants.

Read More thumbnail image: Journal Highlight Improvements in phenotype studies of hemoglobin disorders brought by advances in reversed-phase chromatography of globin chains

Green HPLC: New methods for multicomponent pharmaceuticals

Date: Apr 13, 2015

Author: Steve Down

Scientists in Egypt have applied green principles to the HPLC analysis of four pharmaceutical formulations containing multiple active ingredients, to illustrate that conventional procedures can be replaced with no detrimental effects on method performance.

Read More thumbnail image: Green HPLC New methods for multicomponent pharmaceuticals

Cannabinoid calamity: Novel interferent identified in serum of cannabis users by LC/MS

Date: Mar 30, 2015

Author: Steve Down

An unknown cannabinoid that has been detected in the serum of cannabis users interferes in the LC/MS analysis of THC-COOH, say researchers in Germany, who expressed surprise that it had not been reported previously.

Read More thumbnail image: Cannabinoid calamity Novel interferent identified in serum of cannabis users by LCMS

Journal Highlight: Oxidative degradation of polysorbate surfactants studied by liquid chromatography–mass spectrometry

Date: Mar 23, 2015

Author: separationsNOW

Polysorbate mixtures intended for use in biotherapeutic formulations have been analysed by an LC/MS method using low m/z marker ions that simplified profiling.

Read More thumbnail image: Journal Highlight Oxidative degradation of polysorbate surfactants studied by liquid chromatographymass spectrometry

Bleaching cosmetics and tooth products: Peroxide levels tested by HPLC

Date: Mar 16, 2015

Author: Steve Down

French government scientists have proposed an HPLC method for measuring hydrogen peroxide in teeth bleaching kits and hair cosmetics that could replace the current EC official method that dates to 1982.

Read More thumbnail image: Bleaching cosmetics and tooth products Peroxide levels tested by HPLC

Stirring it up for aflatoxins: Turbulent flow chromatography in dual-column HPLC analysis

Date: Mar 3, 2015

Author: Steve Down

Four aflatoxins in a range of foods have been measured simultaneously by 2D HPLC/MS, using turbulent flow chromatography in the first dimension to speed up the whole analysis.

Read More thumbnail image: Stirring it up for aflatoxins Turbulent flow chromatography in dual-column HPLC analysis

Journal Highlight: Advances in isoflavone profile characterisation using matrix solid-phase dispersion coupled to HPLC/DAD in Medicago species

Date: Feb 23, 2015

Author: separationsNOW

A matrix solid-phase dispersion extraction procedure coupled to HPLC/DAD was used as an alternative methodology to evaluate isoflavone profiles in several Medicago species.

Read More thumbnail image: Journal Highlight Advances in isoflavone profile characterisation using matrix solid-phase dispersion coupled to HPLCDAD in Medicago species
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