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The walrus and the chromatographer: LC-MS/MS of steroids in bone

Date: Oct 1, 2018

Author: Ryan De Vooght-Johnson

Examination of the steroid hormone levels in animals from past generations can give useful information on stress and reproduction. Researchers from the University of Alaska Fairbanks and Purdue University (West Lafayette, Indiana) used LC-MS/MS to detect steroid hormones in bones from walruses that had died recently and also those from ancient specimens.

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Processed purée pongs prevented by LC-MS

Date: Sep 27, 2018

Author: Ryan De Vooght-Johnson

Tomato products can be spoiled, giving rise to unpleasant tastes, by various species of Alicyclobacillus bacteria. Researchers from The Experimental Station for the Food Processing Industry in Parma, Italy, used LC-MS to identify specific compounds linked to the growth of these bacteria in tomato purée.

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Journal Highlight: A rapid method to determine nine natural cannabinoids in beverages and food derived from Cannabis sativa by liquid chromatography coupled to tandem mass spectrometry on a QTRAP 4000

Date: Sep 21, 2018

Author: separationsNOW

An LC/MS/MS method has been developed for measuring nine phytocannabinoids in food and beverages derived from hemp seeds, flour and oil.

Read More thumbnail image: Journal Highlight A rapid method to determine nine natural cannabinoids in beverages and food derived from Cannabis sativa by liquid chromatography coupled to tandem mass spectrometry on a QTRAP 4000

Mutated genes are plain to see using dHPLC

Date: Sep 12, 2018

Author: Ryan De Vooght-Johnson

Denaturing HPLC (dHPLC) uses ion-pair reversed-phase HPLC to separate homoduplex and heteroduplex DNA molecules, but typically produces large amounts of data. Scientists from the Institute of Technology and Higher Education (Monterrey, Mexico) and the ‘ISSSTESON’ institute (Hermosillo, Mexico) used dHPLC in combination with principal component analysis (PCA) and box plots to detect DNA point mutations associated with cancer.

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Paracetamol pulverises proteins via para-benzoquinone metabolite

Date: Aug 30, 2018

Author: Ryan De Vooght-Johnson

Paracetamol overdose can cause severe liver damage due to the binding of the reactive metabolite NAPQI to liver proteins. Scientists from two Canadian institutions, the University of Quebec at Montreal and the University of Alberta (Edmonton), used LC-MS/MS to quantify the adduct formed from NAPQI and human serum albumen (HSA), this being a useful biomarker for the amount of NAPQI bound to liver proteins.

Read More thumbnail image: Paracetamol pulverises proteins via para-benzoquinone metabolite

Journal Highlight: Quantification of hydrolyzed peptides and proteins by amino acid fluorescence

Date: Aug 20, 2018

Author: separationsNOW

HPLC using ion‐pairing coupled to fluorescence detection has been used to measure tyrosine, phenylalanine, and tryptophan from hydrolysed peptides without requiring prior derivatization, which will be useful in drug discovery.

Read More thumbnail image: Journal Highlight Quantification of hydrolyzed peptides and proteins by amino acid fluorescence

More alcohol is seen to make your LC green

Date: Aug 20, 2018

Author: Ryan De Vooght-Johnson

HPLC traditionally uses environmentally harmful solvents, such as acetonitrile, although less harmful alternatives often work just as well. Scientists from the Ahram Canadian University (near Cairo) and Ain Shams University (Cairo) used experimental design to optimise two HPLC systems, both using ethanol and buffer solution, for the analysis of cardiovascular drugs, examining the results using two different green evaluation tools.

Read More thumbnail image: More alcohol is seen to make your LC green

DBS and LC-MS crack cocaine analysis

Date: Aug 9, 2018

Author: Ryan De Vooght-Johnson

The accurate analysis of cocaine and its metabolites in blood samples is important for both health professionals and law enforcement agencies. Scientists from the University of Pavia, Italy, devised a new method of analysing dried blood spots (DBSs) containing cocaine or its metabolites by LC-MS/MS, applying it to post-mortem blood samples.

Read More thumbnail image: DBS and LC-MS crack cocaine analysis

Journal Highlight: Comparative analysis of marketed factor VIII products: Recombinant products are not alike vis‐a‐vis soluble protein aggregates and subvisible particles

Date: Jul 23, 2018

Author: separationsNOW

The critical quality parameters of recombinant factor VIII products such as the concentrations of soluble protein aggregates and subvisible particles have been compared by HPLC-SEC and flow cytometry under intended use and use‐relevant stress conditions.

Read More thumbnail image: Journal Highlight Comparative analysis of marketed factor VIII products Recombinant products are not alike visavis soluble protein aggregates and subvisible particles

Monoclonal mixtures measured in plasma

Date: Jul 20, 2018

Author: Ryan De Vooght-Johnson

The use of animals in pharmaceutical pharmacokinetic studies needs to be minimised for both ethical and financial reasons. Scientists from Chungnam National University, Daejeon, South Korea, investigated the use of multiple dosing (known as cassette dosing) for following the pharmacokinetics of four monoclonal antibodies by means of LC-MS.

Read More thumbnail image: Monoclonal mixtures measured in plasma
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