Waters introduces new LC columns for antibody analysis

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  • Published: Jan 31, 2018
  • Source: Waters Corporation
  • Suppliers: Waters Corporation
  • Channels: HPLC / Base Peak
thumbnail image: Waters introduces new LC columns for antibody analysis

Waters Corporation has introduced BioResolve RP mAb Polyphenyl 450 angstrom 2.7 micron solid core columns intended for the reversed-phase analysis of intact or sub-unit-digested monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) using LC-UV and LC-MS.

“A segment of the scientist community regards mAb and ADC separations as overly complex. The causes of their frustrations are many and they include poor data quality, degradation of the target mAbs, poor recovery, excessive carryover and erratic results due to column-to-column irreproducibility,” said Erin Chambers, vice president, chemistry, Waters Corporation. “To address these issues, we tasked our research scientists to engineer a column that would raise the bar for separation performance and take the hassle out of the measurement process. Our ultimate goal is to enable biopharmaceutical scientists to generate consistent results that meet international regulatory requirements to confirm product efficacy and safety.”

Jacquelynn Smith, a member of Pfizer’s Analytical Research and Development Department in St. Louis, Missouri, US. has evaluated the BioResolve RP mAb Polyphenyl column. “The newly introduced BioResolve RP mAb Polyphenyl wider pore column provides superior recovery, separation and resolution of therapeutic monoclonal antibody subunits and domains, as compared to other RP C4 columns on the market,” she said. “This resulted in higher quality product profiles for mAbs and antibody drug conjugates, especially when hydrophobic subunits and domains were encountered. The BioResolve RP mAb Polyphenyl column addresses the key challenges of mAb and ADC subunit/domain analysis, helps toward right-first time analyses, and will be a valuable addition to our characterization toolbox.”

The combination of the BioResolve RP mAb Polyphenyl column 450 angstrom solid-core particle design and proprietary polyphenyl ligand bonding makes it especially well-suited for reversed phase separations of intact mAbs, IdeS-generated fragments and similarly sized proteins. These columns establish a new industry standard for separation selectivity, efficiency, sample recovery and low injection-to-injection carryover even when operated at lower temperatures.

The BioResolve RP mAb Polyphenyl 450 angstrom 2.7 micron columns are available in lengths of 50mL, 100mL or 150mL and internal diameters of 2.1mL or 4.6mL. This make them compatible with UltraPerformance Liquid Chromatography (UPLC), Ultra-High Performance Liquid Chromatography (UHPLC) or High Performance Liquid Chromatography (HPLC) separations platforms, thus facilitating easy method transfer between discovery, development and manufacturing/QC applications.
 

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