Journal Highlight: Determination of urine albumin by new simple high-performance liquid chromatography method

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  • Published: Dec 19, 2016
  • Author: separationsNOW
  • Channels: HPLC
thumbnail image: Journal Highlight: Determination of urine albumin by new simple high-performance liquid chromatography method

A simple HPLC method has been developed for the determination of albumin in patients' urine samples without coeluting proteins, proving to be more sensitive than immunoturbidimetry.

Determination of urine albumin by new simple high-performance liquid chromatography method

Journal of Clinical Laboratory Analysis, 2016, 30, 1226-1231
Eva Klapkova, Magdalena Fortova, Richard Prusa, Libuse Moravcova and Karel Kotaska

Abstract: A simple high-performance liquid chromatography (HPLC) method was developed for the determination of albumin in patients' urine samples without coeluting proteins and was compared with the immunoturbidimetric determination of albumin. Urine albumin is important biomarker in diabetic patients, but part of it is immuno-nonreactive. Albumin was determined by high-performance liquid chromatography (HPLC), UV detection at 280 nm, Zorbax 300SB-C3 column. Immunoturbidimetric analysis was performed using commercial kit on automatic biochemistry analyzer COBAS INTEGRA® 400, Roche Diagnostics GmbH, Manheim, Germany. The HLPC method was fully validated. No significant interference with other proteins (transferrin, α-1-acid glycoprotein, α-1-antichymotrypsin, antitrypsin, hemopexin) was found. The results from 301 urine samples were compared with immunochemical determination. We found a statistically significant difference between these methods (P = 0.0001, Mann–Whitney test). New simple HPLC method was developed for the determination of urine albumin without coeluting proteins. Our data indicate that the HPLC method is highly specific and more sensitive than immunoturbidimetry.

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