Journal Highlight: LogP measurement of a highly hydrophobic properfume: evaluation of extrapolation of RP-HPLC results and impact of chemical moieties on accuracy

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  • Published: May 23, 2016
  • Author: separationsNOW
  • Channels: HPLC
thumbnail image: Journal Highlight: LogP measurement of a highly hydrophobic properfume: evaluation of extrapolation of RP-HPLC results and impact of chemical moieties on accuracy
The LogP of properfume Haloscent®D was measured by RP-HPLC using an indirect method involving its relationship with the retention factor and careful selection of suitable standards.

LogP measurement of a highly hydrophobic properfume: evaluation of extrapolation of RP-HPLC results and impact of chemical moieties on accuracy

Flavour and Fragrance Journal, 2016, 31, 235-240
F. Begnaud, J.-P. Larcinese, P. Fankhauser and U. Maddalena

Abstract: The n-octanol/water partition coefficient (LogP) of a chemical substance is a key parameter for assessing its potential (eco)-toxicological effects and environmental fate. To measure the LogP of properfume Haloscent®D (expected LogP > 9), no direct measurement technique can be applied and the reliability of in silico models is impaired by the absence of robust datasets in the high LogP range. Indirect method using reversed-phase-HPLC (RP-HPLC) is promising but we demonstrate that the lack of suitable standards lead to significant discrepancy and poor accuracy. Extrapolation of the correlation between the RP-HPLC retention factor (logk) of low-carbon chain homologues and their LogP measured by slow-stir method has been studied. To select the most suitable standards, the impact of specific moiety representative of Haloscent®D (linear alkyl chain, delta-damascone moiety, thioether function) on the logk vs. estimated LogP has been checked on different molecules and it was demonstrated that the alkyl chain had the most impact on this correlation. The stability in the molecular conformation of Haloscent®D homologue series was also verified to ensure that no folding occurred as from a certain alkyl chain length, and that consequently extrapolation is feasible up to Haloscent®D. Accuracy of the extrapolated LogP value was calculated from the prediction interval of the logk vs. LogP regression. The LogP of the Haloscent®D was concluded to be 9.7 ± 0.5.

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