Journal Highlight: GC-MS analysis of various phytoestrogens in health functional foods

Skip to Navigation

Ezine

  • Published: May 15, 2017
  • Author: separationsNOW
  • Channels: Gas Chromatography
thumbnail image: Journal Highlight: GC-MS analysis of various phytoestrogens in health functional foods

The contents of 21 phytoestrogens in commercial health functional foods in South Korea sold to alleviate menopausal symptoms have been measured by GC/MS.

GC-MS analysis of various phytoestrogens in health functional foods

Bulletin of the Korean Chemical Society, 2017, 38, 448-458
Yu Ra Kim, Hee Soo Pyo, Bong Chul Chung, Myeong Hee Moon and Jeongae Lee

Abstract: This study aimed to measure phytoestrogen contents in health functional foods (HFFs) to alleviate menopausal symptoms that are commercially available in South Korea. A sensitive and selective method using gas chromatography–mass spectrometry (GC-MS) for the monitoring of 21 phytoestrogens was developed and fully validated. Phytoestrogens were extracted by liquid–liquid extraction using methyl tert-butylether as the solvent. Food samples were enzymatically hydrolyzed with β-glucuronidase/arylsulfatase. Before GC-MS, the analytes were derivatized to their trimethylsilyl derivatives by incubation with N,O-bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane for 30 min at 80°C. The calibration curves showed good linearity (r2 > 0.99) in the range of interest for each analyte. The limits of detection and quantification of the 21 phytoestrogens were in the ranges of 0.5–20 and 1–50 ng/mL, respectively. The intra- and inter-day precisions were all below 15.7%. These multivariate procedures were efficient in determining the optimal conditions using peak heights as responses.

  • This paper is free to view for all users registered on separationsNOW.com until the end of July 2017.
    After this time, you can purchase it using Pay-Per-View on Wiley Online Library.

Follow us on Twitter!

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Copyright Information

Interested in spectroscopy? Visit our sister site spectroscopyNOW.com

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved