Journal Highlight: Quantitative analysis of bioactive compounds from aromatic plants by means of dynamic headspace extraction and multiple headspace extraction-GC/MS

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  • Published: May 16, 2016
  • Author: separationsNOW
  • Channels: Gas Chromatography
thumbnail image: Journal Highlight: Quantitative analysis of bioactive compounds from aromatic plants by means of dynamic headspace extraction and multiple headspace extraction-GC/MS
Seven bioactive monoterpenes in 4 aromatic plants (sage, cardamom, lavender, and rosemary) were quantified in liquid extracts and directly in solid samples by dynamic headspace-GC/MS and multiple headspace extraction-GC/MS, respectively.

Quantitative analysis of bioactive compounds from aromatic plants by means of dynamic headspace extraction and multiple headspace extraction-gas chromatography-mass spectrometry

Journal of Food Science, 2016, 81, C867-C873
Jone Omar, Maitane Olivares, Ibone Alonso, Asier Vallejo, Oier Aizpurua-Olaizola and Nestor Etxebarria

Abstract: Seven monoterpenes in 4 aromatic plants (sage, cardamom, lavender, and rosemary) were quantified in liquid extracts and directly in solid samples by means of dynamic headspace-gas chromatography-mass spectrometry (DHS-GC-MS) and multiple headspace extraction-gas chromatography-mass spectrometry (MHSE), respectively. The monoterpenes were 1st extracted by means of supercritical fluid extraction (SFE) and analyzed by an optimized DHS-GC-MS. The optimization of the dynamic extraction step and the desorption/cryo-focusing step were tackled independently by experimental design assays. The best working conditions were set at 30 °C for the incubation temperature, 5 min of incubation time, and 40 mL of purge volume for the dynamic extraction step of these bioactive molecules. The conditions of the desorption/cryo-trapping step from the Tenax TA trap were set at follows: the temperature was increased from 30 to 300 °C at 150 °C/min, although the cryo-trapping was maintained at −70 °C. In order to estimate the efficiency of the SFE process, the analysis of monoterpenes in the 4 aromatic plants was directly carried out by means of MHSE because it did not require any sample preparation. Good linearity (r2 > 0.99) and reproducibility (relative standard deviation % <12) was obtained for solid and liquid quantification approaches, in the ranges of 0.5 to 200 ng and 10 to 500 ng/mL, respectively. The developed methods were applied to analyze the concentration of 7 monoterpenes in aromatic plants obtaining concentrations in the range of 2 to 6000 ng/g and 0.25 to 110 μg/mg, respectively.

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