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Journal Highlight: An integrated chemical cytometry method: Shining a light on Akt activity in single cells

Date: Nov 14, 2016

Author: separationsNOW

Researchers in the US have developed an integrated analysis system that utilizes a light-programmable, cell-permeable reporter with capillary electrophoresis to study the activity of the kinase Akt in single cells.

Read More thumbnail image: Journal Highlight An integrated chemical cytometry method Shining a light on Akt activity in single cells

Journal Highlight: Multiplexed capillary electrophoresis as analytical tool for fast optimization of multi-enzyme cascade reactions – synthesis of nucleotide sugars

Date: Oct 24, 2016

Author: separationsNOW

German researchers have for the first time demonstrated the use of multiplexed capillary electrophoresis as a fast analytical tool for the optimization of nucleotide sugar synthesis with multi-enzyme cascade reactions.

Read More thumbnail image: Journal Highlight Multiplexed capillary electrophoresis as analytical tool for fast optimization of multi-enzyme cascade reactions  synthesis of nucleotide sugars

A blot on the beads: Western blotting with a bead-based immunoassay

Date: Oct 18, 2016

Author: Jon Evans

By combining Western blotting with a bead-based immunoassay, German scientists have developed a novel proteomics technique that can conduct 200 separate analyses using the same amount of sample as required for a single conventional Western blot.

Read More thumbnail image: A blot on the beads Western blotting with a bead-based immunoassay

Journal Highlight: Possibilities and limitations of 2DE-based analyses for identifying low-abundant tumor markers in human serum and plasma

Date: Oct 10, 2016

Author: separationsNOW

This review explores the use of 2D gel electrophoresis to discover cancer biomarkers in serum/plasma and also proposes an optimized experimental workflow for detecting cancer-specific protein markers of low abundance.

Read More thumbnail image: Journal Highlight Possibilities and limitations of 2DE-based analyses for identifying low-abundant tumor markers in human serum and plasma

Journal Highlight: Differential protein expression in the midgut of Culex quinquefasciatus mosquitoes induced by the insecticide temephos

Date: Sep 26, 2016

Author: separationsNOW

Scientists in Brazil and the US used 2D gel electrophoresis to investigate the effect of the insecticide temephos on the expression of midgut proteins in a mosquito.

Read More thumbnail image: Journal Highlight Differential protein expression in the midgut of Culex quinquefasciatus mosquitoes induced by the insecticide temephos

Pull to conform: Analyzing protein conformation and activity with kinetic CE

Date: Sep 19, 2016

Author: Jon Evans

By combining a form of capillary electrophoresis known as kinetic CE with UV detection and ion mobility-mass spectrometry, Canadian scientists have developed the first technique able to monitor protein conformation and activity at the same time.

Read More thumbnail image: Pull to conform Analyzing protein conformation and activity with kinetic CE

Journal Highlight: Electrochemical determination of salivary N-acetylneuraminic acid by miniaturized capillary electrophoresis coupled with sample stacking

Date: Sep 12, 2016

Author: separationsNOW

Chinese scientists have developed a novel capillary electrophoresis method for determining a specific sialic acid in human saliva.

Read More thumbnail image: Journal Highlight Electrochemical determination of salivary N-acetylneuraminic acid by miniaturized capillary electrophoresis coupled with sample stacking

Journal Highlight: Performance assessment of protein electrophoresis by using polyacrylamide hydrogel with porous structure modified with SDS micelles as template

Date: Aug 22, 2016

Author: separationsNOW

Chemical engineers from Brazil and the US have synthesized polyacrylamide hydrogels in the presence of sodium dodecyl sulfate micelles in order to modify the internal porous microstructure of electrophoresis gels.

Read More thumbnail image: Journal Highlight Performance assessment of protein electrophoresis by using polyacrylamide hydrogel with porous structure modified with SDS micelles as template

Press print to separate: Fabricating CE microchips with 3D printing

Date: Aug 15, 2016

Author: Jon Evans

For the first time, scientists in the US have used 3D printing to produce a working capillary electrophoresis microchip.

Read More thumbnail image: Press print to separate Fabricating CE microchips with 3D printing

Journal Highlight: Selection of lactic acid bacteria strains for the hydrolysis of allergenic proteins of wheat flour

Date: Aug 8, 2016

Author: separationsNOW

Polish researchers have used gel electrophoresis to help identify strains of lactic acid bacteria capable of hydrolyzing/modifying allergenic proteins in wheat flour.

Read More thumbnail image: Journal Highlight Selection of lactic acid bacteria strains for the hydrolysis of allergenic proteins of wheat flour
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