AATI’s pulsed-field CE system wins R&D 100 Award

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  • Published: Nov 29, 2016
  • Author: Jon Evans
  • Source: AATI
  • Channels: Electrophoresis

Advanced Analytical Technologies Inc (AATI) has received an R&D 100 Award from R&D Magazine for its FEMTO Pulse automated pulsed-field capillary electrophoresis (CE) instrument. As the only automated pulsed-field (CE) system on the market, the FEMTO Pulse won the award for its ability to separate and analyze large nucleic acid fragments up to 165,000 base pairs with high efficiency and sensitivity.

The R&D 100 Awards recognize the 100 most technologically significant products introduced into the marketplace over the past year. Commonly known as the ‘Oscars of Invention’, the awards have recognized pioneering technology for more than 50 years.

Successful next-generation sequencing (NGS) is reliant upon the construction of quality NGS libraries. Synthesis of these libraries, from short-read sequencing to the latest long-read sequencing applications, depends on several critical quality control (QC) checkpoints. While sequencing technology has advanced, QC methods have stagnated, limiting their application in new NGS technologies. The FEMTO Pulse fills a niche in NGS workflows, as it is capable of quantifying and qualifying challenging samples, from initial QC steps to final library validation.

“NGS systems are now starting to analyze fragments over 50,000 base pairs, and most labs tell me they have little starting material for preparing a library and need to be able to assess its size. These are precious samples,” said Steve Siembieda, AATI’s vice president of commercialization.

With sequencing moving to larger and larger fragments, AATI saw a need for a completely new analytical instrument design. The result is the FEMTO Pulse, the first device of its kind to feature a parallel CE platform with a pulsed-field power supply and enhanced optical sensors, allowing it to push the boundaries of nucleic acid detection and fragment sizing.

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