Western Blot Visual Protocol: Phase 1: Sample Preparation

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  • Published: Mar 1, 2013
  • Source: Novus Biologicals
  • Channels: Sample Preparation
thumbnail image: Western Blot Visual Protocol: Phase 1: Sample Preparation

Novus Biologicals Visual Protocols: In phase 1 of the western blot procedure, you will learn how to prepare your samples before loading them into a gel. Here we isolate protein from cultured cells, quantify total protein concentrations with a BCA assay, add loading buffer to the sample, and heat the sample. Additional help can be found in the support section of http://www.novusbio.com.


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