Thermo Fisher offers single system for biopharmaceutical characterization

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  • Published: Jun 7, 2016
  • Author: Jon Evans
  • Source: Thermo Fisher Scientific
  • Suppliers: Thermo Fisher Scientific
  • Channels: HPLC / Base Peak

Researchers performing biopharmaceutical characterization can now benefit from a single system from Thermo Fisher Scientific that provides three powerful workflows for the complete characterization of biotherapeutics.

The new Thermo Scientific Q Exactive BioPharma MS/MS Hybrid Quadropole-Orbitrap mass spectrometer takes full advantage of the high-resolution, accurate-mass (HRAM) capabilities of the Orbitrap mass analyzer to enable three key protein characterization workflows. These are denatured and native MS intact analysis, subunit and top/middle-down analysis, and peptide mapping. Combined with recommended sample preparation and analysis methods based on Thermo Scientific reagents, columns, UHPLC and data processing technologies, the Q Exactive BioPharma constitutes a complete solution for the rigorous structural characterization of biotherapeutic compounds.

“Researchers require multiple MS techniques to perform the experiments essential for biopharmaceutical structure characterization,” said Jonathan Josephs, director, pharma/biopharma marketing, life sciences mass spectrometry at Thermo Fisher. “We now offer a single instrument that encompasses all of our leading technologies into one system, which can be used to analyze antibody-based biologics at the intact, subunit and peptide levels – ultimately providing an efficient route to high quality results.”

The Thermo Scientific Q Exactive BioPharma features a new high mass range (HMR) mode. This adds the ability for exceptional intact monoclonal antibody and antibody-drug conjugate (ADC) analysis, under both native intact and denatured intact conditions, to the existing capabilities for subunit top/middle-down and peptide mapping analysis.

When combined with Thermo Scientific BioPharma Finder Software, the technology is a powerful solution for biotherapeutic characterization. The integrated workflow-driven interface enables automated processing of data from peptide, subunit and intact protein analyses. BioPharma Finder uses two deconvolution algorithms to take full advantage of the ultra-high-quality, high-resolution accurate mass (HRAM) data produced by Orbitrap-based mass spectrometers to provide superb accuracy for intact mass measurements. This powerful software can also be used to accurately characterize disulfide bond linkages, detect low-level impurities, and automate the relative quantitation and reporting of sequence variants and post-translational modifications.

To further complete the solution, the new Thermo Scientific Vanquish Flex Binary UHPLC system adds a binary solvent delivery option in the 1000 bar (15,000 psi) performance range. The system is built for high-speed, fast gradient applications and benefits from the Vanquish platform’s inherent outstanding retention time reproducibility. The Vanquish Flex Binary UHPLC system is the solution for pharmaceutical and biopharmaceutical laboratories performing qualitative and quantitative LC or LC-MS analysis. Ease-of-use and innovative technologies that are robust by design are making UHPLC widely accessible, and allow easy and effective method transfer from established systems and applications.

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