Journal Highlight: Updating chromatographic predictions by accounting ageing for single and tandem columns

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  • Published: Jun 11, 2018
  • Author: separationsNOW
  • Channels: Laboratory Informatics / HPLC
thumbnail image: Journal Highlight: Updating chromatographic predictions by accounting ageing for single and tandem columns

Models for predicting chromatographic behaviour on single and coupled columns which have aged have been adapted to correct the time and peak profiles.

Updating chromatographic predictions by accounting ageing for single and tandem columns

Journal of Separation Science, 2018, online
Tamara Alvarez‐Segura, J.R. Torres‐Lapasió, María Celia García‐Alvarez‐Coque

Abstract: The most efficient optimisation methodologies in liquid chromatography are based on the modelling and prediction of the chromatographic behaviour for each compound in the sample. However, when the column suffers some ageing after the modelling process, predictions may differ significantly from the actual separation. Repeating the modelling is especially troublesome when several columns are involved, as is the case of coupled columns. We propose a shortcut to correct the time and peak profiles in these situations, after evaluating the effects of ageing. The original models are corrected by introducing parameters accounting for column ageing, obtained using the data of a small subset of compounds from those used to model the brand‐new column. The ageing parameters are fitted from the discrepancies between the data predicted with the original retention models for the brand‐new column and the experimental data measured for the aged column. The approach was developed and tested to predict the chromatographic behaviour of 15 sulfonamides, analysed with individual and tandem columns, using isocratic and gradient elution. Chromatograms more in line with the aged column performance were predicted. The agreement between predictions and experimental data in the aged columns was excellent.

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