An overview of the basics of proteomics

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Education Article

  • Published: Sep 29, 2005
  • Channels: Proteomics & Genomics
thumbnail image: An overview of the basics of proteomics

Industrial Proteomics coverIn this free chapter from Industrial Proteomics: Applications for Biotechnology and Pharmaceuticals Daniel Figeys gives a basic overview of proteomics, including:

  • Introduction
    • Deoxyribonucleic Acid to Ribonucleic Acid
    • Ribonucleic Acid to Protein
  • Proteome Handling in Classical and Functional Proteomics
  • Protein Purification
    • Cell Culture
    • Other Source of Samples
    • Subpopulation of Cells
    • Subcellular Components
  • Protein Separation by Two-Dimensional Gel Electrophoresis
    • Principles of Two-Dimensional Gel Electrophoretic Separation
    • Detection of Proteins Separated by Two-Dimensional Gel Electrophoresis
    • Software to Handle Two-Dimensional Gel Electropherogram
  • Potein Processing
    • Blot Digestion of Proteins
    • In-gel Digestion of Proteins
  • Protein Identification
    • Edman Degradation
    • Protein Identification by Mass Spectrometry
    • Hybrid Instruments
    • Bioinformatics for the Identification of Proteins Based on MS/MS
  • Current Challenges in Proteomics
  • Classical Proteomics
    • Gel-Free Analysis: An Alternative Differential Display
    • Fourier Transofrm Mass Spectrometery for Gel-Free Analysis and
      Single-Peptide-Based Identification

Click here to download this chapter (PDF format, 62 Pages, 5Mb)


This chapter is taken from Industrial Proteomics: Applications for Biotechnology and Pharmaceuticals, edited by Daniel Figeys

This book covers both basic elements and the state-of-the-art in applications of proteomics. The first section gives an introduction to the field, from differential approaches to functional proteomics, including yeast two-hybrid, mass spectrometry, mapping of post-translational modifications, and NMR spectroscopy. The second section offers a complete picture of industry studies in proteomics for human health and biotechnology applications. The final section addresses the future, looking at the most promising areas such as protein arrays and the integration of genomic and proteomic tools.

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