Rapid analysis of traditional Chinese medicine Pinellia ternata by microchip electrophoresis with electrochemical detection

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EarlyView Article

  • Published: Dec 18, 2017
  • Author: Tsung‐Ting Shih, Hui‐Ling Lee, Show‐Chuen Chen, Chih‐Yuan Kang, Ren‐Shang Shen, Yi‐An Su
  • Journal: Journal of Separation Science

Abstract

Traditional Chinese herbal medicine has long enjoyed the reputation of the world's most advanced system of natural medicine. Pinellia ternata is one of the most commonly used herbs in the traditional Chinese medical science. In this study, five representative ingredients of Pinellia ternata guanosine, methionine, glycine, 3,4‐dihydroxybenzaldehyde, and homogentisic acid, were assayed using simple derivatization procedures. Under optimized experimental condition, five analytes in Pinellia ternata were rapidly separated and detected using microchip electrophoresis, affording the benefits of speed, minimal sample requirements, and sensitive on‐the‐chip electrochemical detection, in 5 min with linearity over a concentration of 20–500 μM (R= 0.994) with nearly complete recovery (95.6–98.5%).

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