.... http://www.separationsNOW.com/coi/cda/landing.cda?type=Feature&chId=6 http://www.separationsNOW.com/coi/cma/download.cma?imageType=RssLogo&contentType=Feature&siteId=2&chId=6 http://www.separationsnow.com/ http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26662&type=Feature&page=1 A record number of ancient proteins have been isolated and identified from the femur of a 43,000 year-old woolly mammoth that had been preserved in permafrost, using specially developed techniques to minimise contamination along with high-resolution mass spectrometry. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26613&type=Feature&page=1 The performance of Cibacron Blue dye (HiTrapBlue or Affigel Blue) in depleting albumin from plasma, as a pre-treatment for biomarker searching in the low-abundance proteome, is assessed. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26609&type=Feature&page=1 The unacceptably low survival rate of cloned animals might be related to the aberrant expression of proteins associated with oxidative damage, apoptosis and senescence, say Korean scientists. The restoration of normal placental protein levels could increase the efficiency and help to save endangered species. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26497&type=Feature&page=1 An unknown black market product seized by German customs agents was found to contain fibroblast growth factor, say European scientists. The protein, which has not been given full clinical approval, was probably intended for illegal sports doping. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26460&type=Feature&page=1 A novel tentacle-type polymer stationary phase covalently modified with branched polyethyleneimine was developed for peptides and proteins separations by open-tubular CEC, giving high column efficiencies. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26437&type=Feature&page=1 Several innate immunity proteins have been identified in the nasal lavage fluid of small children suffering from a respiratory virus infection, including full-length and truncated versions of an antibacterial protein which were implicated in higher risk factors for infant boys. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26390&type=Feature&page=1 The protein changes occurring in the part of the brain that deals with new memories have been charted during a spatial learning activity by rats in an international study which revealed two discrete change phases within 12 hours of training. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26349&type=Feature&page=1 This review summarizes the technical developments and biological applications of the proteomic reactor over the last decade, in its two major forms of immobilized enzyme reactor and ion exchange-based proteomic reactor. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26303&type=Feature&page=1 A proteomics study following proteins through the brewing process has suggested that the haze found in beers originates predominantly from barley proteins, rather than the hordein proteins in malt that had previously been implicated. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26251&type=Feature&page=1 Diagnosis of obstructive sleep apnoea based on serum protein levels took a step closer following a preliminary proteomic study on affected men in which the expression of some proteins was related to the severity of the condition. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26227&type=Feature&page=1 This review provides an overview of the history, advantages and disadvantages of flow cytometry and focuses on the current applications and future possibilities of flow cytometry in plant pathology. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26185&type=Feature&page=1 The phosphoprotein signatures of archived prostate cancer tissues have been successfully characterised by a gel-free proteomics approach in a pilot study that can form the basis of a biomarker discovery campaign. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26136&type=Feature&page=1 Universal screening of newborn children for sickle cell disease is possible due to the development of a rapid mass spectrometric method designed to detect all types of haemoglobin variant and which can process up to 1000 samples a day. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26072&type=Feature&page=1 A method has been described for the simultaneous isolation of populations of hepatocytes, hepatic stellate cells, Kupffer cells and liver sinusoidal endothelial cells by a combination of collagenase-based density gradient centrifugation and magnetic activated cell sorting. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26042&type=Feature&page=1 One of the risk factors associated with toxic oil syndrome has been identified in proteomics studies as a phenotype of haptoglobin, with a different phenotype affording protection, according to researchers in Spain where the disease struck. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=26005&type=Feature&page=1 Ocean acidification alters the proteome of the larvae of an ecologically important barnacle species, to a small but significant extent. Molecular chaperones and proteins related to energy metabolism and respiration had altered expression levels, hinting at the adopted mechanism for coping with acidification stress. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=25970&type=Feature&page=1 Upstream protein or peptide separation techniques for typical mammalian affinity purification mass spectrometry resulted in a modest increase in the number of unique peptides and additional proteins identified at the cost of increased instrument and handling time. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=25945&type=Feature&page=1 The proteomes of the antennae of two castes of bees reflect their different activities, promoting foraging activity in the forager honeybees and sex pheromone detection in mature drone bees, say researchers in China. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=25856&type=Feature&page=1 An ELISA method for the prediction of acute cellular rejection in liver transplant patients with high sensitivity and specificity has been derived on the basis of proteins identified from a proteomic study of patient serum. http://www.separationsNOW.com/coi/cda/detail.cda?chId=6&id=25808&type=Feature&page=1 Label-free liquid chromatography mass spectrometry (LC-MS<sup>E</sup>) profiling was used to show that non-targeted proteins were also removed during immunodepletion on a top-20 column, a total of 147 proteins being depleted.